β‐ecdysone promotes osteogenic differentiation of bone marrow mesenchymal stem cells

Abstract

Background β‐ecdysone (βEcd) has numerous pharmacological effects, although its role in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) has not yet been explored. Methods In cell experiments, BMSCs were induced to differentiate by osteogenic induction medium (OIM) or βEcd. In animal experiments, an osteonecrosis of the femoral head (ONFH) rat model was established using lipopolysaccharide plus methylprednisolone and treating the rats with βEcd. The osteogenic differentiation capacity of human BMSCs (hBMSCs) was analyzed by alkaline phosphatase and alizarin red S staining. Histopathological changes in rat femoral head tissues were observed by hematoxylin and eosin staining. The expression levels of RUNX2, COL1A1, OCN and phosphorylated Akt in BMSCs from rat femoral head tissues were measured by a quantitative real‐time polymerase chain reaction or western blot analysis. Results Alkaline phosphatase activity and calcium nodules in the βEcd‐treated BMSC group dose‐dependently increased compared to those in the control and OIM groups. The hematoxylin and eosin staining results indicated that femoral head tissues of ONFH rats showed typical osteonecrosis, which could be ameliorated by βEcd. Western blot, quantitative real‐time polymerase chain reaction and immunohistochemistry assays demonstrated that the expression levels of RUNX2, COL1A1 and OCN in hBMSCs and femoral head tissue models were obviously increased after βEcd treatment, and phosphoinositide 3‐kinase and Akt phosphorylation were also increased. Conclusions βEcd may be beneficial for the recovery of ONFH patients by accelerating osteogenic differentiation of BMSCs, which may be a novel therapy for related diseases.

Integrated Metagenomic and Metabolomic Analyses of the Effects of Total Flavonoids of Rhizoma Drynariae on Reducing Ovariectomized-Induced Osteoporosis by Regulating Gut Microbiota and Related Metabolites

“…Osteopontin, a bone matrix protein involved in the regulation of absorption, can be inhibited by D-tubocurarine, and then inhibit OP induced by nicotine 38 . β-Ecdysone has a protective effect on bone and can promote the osteogenic differentiation of bone marrow mesenchymal stem cells 39 . Furthermore, the results of metabolic pathway analysis revealed several metabolic pathways such as phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis and so on might play an important role in TFRD against OP.…”

Cytology, function and dynamics of stem and progenitor cells in decapod crustaceans

“…In mammals, 20‐hydroxyecdysone exerts anabolic effects via increase of muscle fibres and activation of satellite cells (Parr et al ., 2015; Tóth et al ., 2008). Moreover, 20‐hydroxyecdysone can induce bone morphogenetic protein‐2‐dependent osteogenic differentiation through the extracellular signal‐regulated kinase (ERK) pathway in human periodontal ligament stem cells (Jian et al ., 2013) and promote osteogenic differentiation of human bone marrow mesenchymal stem cells (You & Xu, 2020), suggesting a potential use in regenerative medicine. In mammals, 20‐hydroxyecdysone may be bound to some extent by the sex steroid receptors.…”

“…Stem cells are of increasing interest in biology and medicine because they play key roles in development, tissue homeostasis, regeneration, ageing and diseases (e.g. Schultz & Sinclair, 2016;Xia et al, 2018;Kus ¸o glu & Biray Avcı, 2019;Zakrzewski et al, 2019). They are relatively well investigated in the potentially immortal cnidarians and determinately growing insects and mammals (e.g.…”

Histone H3K9 demethylase JMJD2B/KDM4B promotes osteogenic differentiation of bone marrow-derived mesenchymal stem cells by regulating H3K9me2 on RUNX2

“…(2) Hormone. Like insulin ( Zhang et al, 2020 ), in addition, there are some xenogeneic hormones that also play a role in inducing differentiation to a certain extent ( You & Xu, 2020 ). (3) Biomaterials.…”