β-Ecdysone has bone protective but no estrogenic effects in ovariectomized rats

Seidlová-Wuttke, Dana; Christel, David Benjamin; Kapur, Priya; Nguyen, Ba Tiep; Jarry, Hubertus; Wuttke, W.

doi:10.1016/j.phymed.2010.03.021

Cited by 50 publications

(49 citation statements)

References 47 publications

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“…In addition, seeds of quinoa (Chenopodium quinoa Willd., Amaranthaceae), a functional food and nutraceutical, have very high contents of PEs (mainly 20E), 4-12 times more than spinach (Kumpun et al 2011), and compounds secreted by intact quinoa seeds into water during initial stages of germination reportedly have anti-diabetic properties, as they can significantly lower fasting blood glucose in obese, hyperglycemic mice (Graf et al 2014). Other therapeutic properties in mammals-including anabolic, performance-enhancing, anti-osteoporotic and wound healing effects-have also been described by various authors (e.g., Slama and Lafont 1995;Kapur et al 2010;Seidlova-Wuttke et al 2010;Syrov and Khushbaktova 1996;Lafont and Dinan 2003).…”

Section: Biological Importance Of Phytoecdysteroidsmentioning

confidence: 72%

Plant ecdysteroids: plant sterols with intriguing distributions, biological effects and relations to plant hormones

Tarkowská

Strnad

2016

Planta

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The present review summarises current knowledge of phytoecdysteroids' biosynthesis, distribution within plants, biological importance and relations to plant hormones. Plant ecdysteroids (phytoecdysteroids) are natural polyhydroxylated compounds that have a four-ringed skeleton, usually composed of either 27 carbon atoms or 28-29 carbon atoms (biosynthetically derived from cholesterol or other plant sterols, respectively). Their physiological roles in plants have not yet been confirmed and their occurrence is not universal. Nevertheless, they are present at high concentrations in various plant species, including commonly consumed vegetables, and have a broad spectrum of pharmacological and medicinal properties in mammals, including hepatoprotective and hypoglycaemic effects, and anabolic effects on skeletal muscle, without androgenic side-effects. Furthermore, phytoecdysteroids can enhance stress resistance by promoting vitality and enhancing physical performance; thus, they are considered adaptogens. This review summarises current knowledge of phytoecdysteroids' biosynthesis, distribution within plants, biological importance and relations to plant hormones.

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Section: Biological Importance Of Phytoecdysteroidsmentioning

confidence: 72%

Plant ecdysteroids: plant sterols with intriguing distributions, biological effects and relations to plant hormones

Tarkowská

Strnad

2016

Planta

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“…Because bEcd is structurally similar to testosterone or estrogen, it is thought to have steroid hormone-like effects including stimulating protein synthesis, promoting carbohydrate and lipid metabolism, alleviating hyperglycemia and hyperlipidemia, having immunologic modulation effects, and protecting endothelial cells from apoptosis. However, it was reported that bEcd did not bind to the estrogen receptor [35], and its pharmacological effects may not involve estrogen receptor activation. To further examine whether hormones play a role in the effects of bEcd on bone, we treated the ORX male and OVX female mice with bEcd.…”

Section: Discussionmentioning

confidence: 99%

β-Ecdysone Augments Peak Bone Mass in Mice of Both Sexes

Dai

Zhang

Zhong

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background One of the strongest predictors for osteoporosis is peak bone mass. Interventions to augment peak bone mass have yet to be developed. b-Ecdysone (bEcd), a natural steroid-like compound produced by arthropods to initiate metamorphosis, is believed to have androgenic effects and so may be used to augment bone mass. Questions/purposes The purpose of this study was to use both male and female (1) gonadal-sufficient; and (2) -insufficient mice to investigate sex differences in terms of bone development and structure after bEcd administration.Methods Two-month-old male and female Swiss-Webster mice were randomized to receive either vehicle or bEcd (0.5 mg/kg) for 3 weeks. In a separate experiment to evaluate the effects of bEcd on sex hormone-deficient mice, gonadectomy was performed in male (orchiectomy [ORX]) and female mice (ovariectomy [OVX]). Shamoperated and the ORX/OVX mice were then treated for 3 weeks with bEcd. Primary endpoints for the study were trabecular bone structure and bone strength. Results In male mice, the trabecular bone volume was 0.18 ± 0.02 in the placebo-treated (PL) and 0.23 ± 0.02 in the bEcd-treated group (p \ 0.05 versus PL); and 0.09 ± 0.01 in the ORX group (p \ 0.05 versus PL) and 0.12 ± 0.01 in the ORX + bEcd group. Vertebral bone strength (maximum load) was 43 ± 2 in PL and 51 ± 1 in the bEcd-treated group (p \ 0.05 versus PL); and 30 ± 4 in the ORX group (p \ 0.05 versus PL) and 37 ± 3 in the ORX + bEcd group. In female mice, trabecular bone volume was 0.23 ± 0.02 in PL and 0.26 ± 0.02 in the bEcd-treated group (p \ 0.05 versus PL); and 0.15 ± 0.01 in the OVX group (p \ 0.05 versus PL) and 0.14 ± 0.01 in the OVX + bEcd group. Maximum load of the vertebrae was 45 ± 2 in PL and 48 ± 4 in the bEcd-treated group; and 39 ± 4 in the OVX group (p \ 0.05 versus PL) and 44 ± 4 in the OVX + bEcd group.

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“…Seidlova-Wuttke et al previously showed that β-ecdysone has an antiosteoporotic effect which does not involve the activation of ER in ovariectomized rats. Thus, olive oil may a exert a similar bone protective effect, with no associated estrogenic effects (21).…”

Section: Discussionmentioning

confidence: 99%

“…In another study, OVX rats were administered oleuropein (a compound extracted from olive leaf) at 2.5, 5, 10 or 15 mg/kg body weight per day for 100 days, and plasma fibrinogen concentration (g/l) was found to be significantly higher in the OVX group compared with the SH group (19). A further increase was observed after inflammation in estrogen-deficient animals, while this marker for inflammation remained unchanged in intact rats (12,20,21). This pattern was partially improved by polyphenol supplementation, as fibrinogen was restored to values measured in OVX animals without talc administration (22).…”

Section: Introductionmentioning

confidence: 99%

Olive oil exhibits osteoprotection in ovariectomized rats without estrogenic effects

Zheng

Huang

Zheng

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. The present study was designed to evaluate the effect of olive oil on bone and uterus in ovariectomized rats. A total of 34 surgically ovariectomized or sham-operated virgin Sprague-Dawley rats were divided into four groups: i) Sham-operated control rats (sham group); ii) Ovariectomized rats (OVX group); iii) Olive oil-supplemented ovariectomized rats (olive group); and iv) Diethylstilbestrol-supplemented ovariectomized rats (E 2 group). At 12 weeks following left ventricular blood sacrificed to detect plasma estradiol (E 2 ), interleukin-1β (IL-1β) and IL-6 levels. Bone mineral density (BMD) of the lumbar spine was evaluated using dual-energy X-ray absorptiometry, and the left femur proximal 1/3 slices were observed using transmission electron microscopy. Uterine wet weight and the uterus index (ratio of uterine wet weight and body weight) were compared, and the uterine endometrium was observed using a light microscope. In the OVX group, serum E 2 was significantly lower and IL-1β and IL-6 levels were significantly higher compared with the sham group. By contrast, serum E 2 levels increased and IL-1β levels decreased in the olive group, but showed no significant difference compared with the sham group. The lumbar spine BMD in the olive group was increased compared with OVX group. Electron microscopy revealed sparse collagen fibers in the OVX group, with decreased density and multi-cavity, showing pathological features of osteoporosis. By contrast, the situation was improved in the E 2 and olive groups, in which organelles such as the rough endoplasmic reticulum, mitochondria and Golgi apparatus were visible and active. Compared with the sham group rats, the uterine wet weight and uterine index decreased in the OVX and olive groups; however, no statistically significant difference was observed in the E 2 group. Furthermore, endometrial hyperplasia was not observed in the olive group, which were apparently different from E 2 group. The present results suggest that olive oil can effectively reduce bone loss in ovariectomized rats, and with no or only mild effects on the uterine endothelium.

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β-Ecdysone has bone protective but no estrogenic effects in ovariectomized rats

Cited by 50 publications

References 47 publications

Plant ecdysteroids: plant sterols with intriguing distributions, biological effects and relations to plant hormones

Plant ecdysteroids: plant sterols with intriguing distributions, biological effects and relations to plant hormones

β-Ecdysone Augments Peak Bone Mass in Mice of Both Sexes

Olive oil exhibits osteoprotection in ovariectomized rats without estrogenic effects

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