β-Cyclodextrin/Triclosan Complex-Grafted Methacrylated Glycol Chitosan Hydorgel by Photocrosslinking via Visible Light Irradiation for a Tissue Bio-Adhesive

Abstract

Accelerating wound healing with minimized bacterial infection has become a topic of interest in the development of the new generation of tissue bio-adhesives. In this study, we fabricated a hydrogel system (MGC-g-CD-ic-TCS) consisting of triclosan (TCS)-complexed beta-cyclodextrin (β-CD)-conjugated methacrylated glycol chitosan (MGC) as an antibacterial tissue adhesive. Proton nuclear magnetic resonance (1H NMR) and differential scanning calorimetry (DSC) results showed the inclusion complex formation between MGC-g-CD and TCS. The increase of storage modulus (G’) of MGC-g-CD-ic-TCS after visible light irradiation for 200 s indicated its hydrogelation. The swollen hydrogel in aqueous solution resulted in two release behaviors of an initial burst and sustained release. Importantly, in vitro and in vivo results indicated that MGC-g-CD-ic-TCS inhibited bacterial infection and improved wound healing, suggesting its high potential application as an antibacterial tissue bio-adhesive.

Visible Light-Cured Antibacterial Collagen Hydrogel Containing Water-Solubilized Triclosan for Improved Wound Healing

“…The complex solution was added to the ColMA hydrogel precursor solution [ 25 ]. The two solutions were mixed until a homogeneous mixture was obtained and photo-cured using riboflavin (12 μM; DPBS) as a photoinitiator for 40 s, and then stored overnight in a refrigerator (2~4 °C) for stabilization.…”

“…The release behavior of TCS from the ColMA/TCS and ColMA/CD-ic-TCS hydrogels was analyzed by ultraviolet–visible spectroscopy (Hitachi U-4100, Mountain View, CA, USA) [ 25 ]. The amount of TCS in both hydrogels was 10 mg.…”

“…At specific time intervals, the cell-seeded hydrogels were treated with CCK-8 (100 μL), and this was followed by a 2 h incubation. After extracting supernatants from the CCK-8-treated wells, the optical densities of the supernatant samples were measured at 450 nm using a microplate reader (SpectraMax ® i3; Molecular Devices, Sunnyvale, CA, USA) [ 25 ].…”

“…Antibacterial activity was evaluated according to a previous study [ 25 , 26 ]. Five hundred microliters of diluted S. aureus and E. coli (1 × 10 6 bacterial/mL) were added to the wells of a 48-well plate containing DuoDERM ® , ColMA, ColMA/TCS, or ColMA/CD-ic-TCS hydrogels (TCS: 1.8 μg), and the plates were incubated at 37 °C for 2 h. Then, 1 mL of PBS was added to each well to resuspend live bacteria.…”

Preparation of Foam Dressings Based on Gelatin, Hyaluronic Acid, and Carboxymethyl Chitosan Containing Fibroblast Growth Factor-7 for Dermal Regeneration

“…Chitosan derivatives, such as carboxymethyl chitosan (CMCS), glycol chitosan, etc., has also been investigated as a wound dressing material because of its high biocompatibility, biodegradability, antimicrobial activity, nontoxicity, and moisturizing capacity [ 9 , 10 ]. These natural polymers have been widely investigated for biomedical applications [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ].…”