Preparation of Foam Dressings Based on Gelatin, Hyaluronic Acid, and Carboxymethyl Chitosan Containing Fibroblast Growth Factor-7 for Dermal Regeneration

Abstract

Wound recovery close to the function of the native skin is the goal of wound healing. In this study, we prepared foam dressings (FDs; 2-GHC-FD-1–9, 5-GHC-FD-1–9, and 10-GHC-FD-1–9) composed of various concentrations of gelatin, hyaluronic acid, and carboxymethyl chitosan, which are chemically interconnected through amide bond formation, for evaluating wound healing. Tensile and cell proliferation tests showed that 2-GHC-FD-1–9 are suitable for wound dressing. For further evaluation, three types of FDs, 2-GHC-FD-1, 2-GHC-FD-4, and 2-GHC-FD-8 were chosen. The results of animal intradermal reactivity, water vapor transmission rate, and absorption rate of the three FDs indicated that 2-GHC-FD-8 is the most appropriate scaffold for wound healing. For wound healing acceleration, various concentrations of fibroblast growth factor-7 (FGF-7) was soaked in 2-GHC-FD-8 (2-GHC-FD-8/F1-6) and evaluated by using scanning electron microscopy, cell proliferation, release behavior, and in vivo animal tests. The FDs showed interconnected porous structures, increased cell proliferation until 8.0 × 10−11 M, controlled release with initial burst within 1 h, and sustained release for 48 h. The results of the animal test showed an appropriate concentration of FGF-7 for wound healing. In addition, 2-GHC-FD-8 is a suitable scaffold for wound healing. Therefore, we suggest that 2-GHC-FD-8/F3 is a useful wound dressing for accelerating wound healing.

Preparation of a Dual-Functional Sulfated Galactofucan Polysaccharide/Poly(vinyl alcohol) Hydrogel to Promote Macrophage Recruitment and Angiogenic Potential in Diabetic Wound Healing

“…Since the fucose content in DF can be obtained in the component analysis, we measured the concentration of fucose to estimate the dissociation of DF. The concentrations of fucose in the solutions were determined by the cysteine hydrochloride–sulfuric acid method with l -fucose as the standard …”

“…The concentrations of fucose in the solutions were determined by the cysteine hydrochloride−sulfuric acid method with L-fucose as the standard. 21 2.5. Cytocompatibility and Hemocompatibility Investigation.…”

Effect of Matrix Metalloproteinase 23 Accelerating Wound Healing Induced by Hydroxybutyl Chitosan

“…In order to construct a multifunctional dressing, we selected chitosan with hemostatic and antibacterial properties to make wound dressing. Chitosan, a derivative of chitin, the second rich polysaccharide in nature, possesses multiple properties, for instance, hemostasis, − antisepsis, , and anti-inflammatory, − promoting angiogenesis. , In addition, chitosan also has admirable physicochemical properties, , which can be used as an ideal carrier system for loading drugs such as cell growth factor − and hyaluronic acid. − In view of some inherent properties, chitosan has already been regarded as a potential material for biomedical applications. − Our previously work exhibited that hydroxybutyl chitosan (HBCS, chemical structure is shown in Scheme ) homogeneously synthesized in alkali/urea aqueous solutions was a brilliant wound dressing. Cytocompatibility studies, using normal human dermal fibroblast (NHDF) cells, demonstrated that HBCS was nontoxic, even promoting the growth of fibroblasts.…”

Fabrication of Sulfated Heterosaccharide/Poly (Vinyl Alcohol) Hydrogel Nanocomposite for Application as Wound Healing Dressing

“…Keeping the wound area moist can be conducive to the micro-environment of wound healing, which is moderately moist, low oxygen, slightly acidic, clean surrounding environment, to accelerate the wound healing [ 8 ]. Hydrogels, foams, and films are used as biomaterials for wound healing and have good water absorption to keep the wound area moist [ 9 , 10 , 11 ]. The hydrogel has a three-dimensional hydrophilic polymer network connected by the covalent bond, hydrogen bond, van der Waals interactions, or other physical or chemical bonds [ 12 ].…”