Effect of Matrix Metalloproteinase 23 Accelerating Wound Healing Induced by Hydroxybutyl Chitosan

Abstract

Skin wounds may cause severe financial and social burden due to the difficulties in wound healing. Original inert dressings cannot meet multiple needs in the process of wound healing. Therefore, the development of materials to accelerate healing progress is essential and urgent. In the previous study, we found that the homogeneously synthesized hydroxybutyl chitosan (HBCS) had an effective performance in promoting wound healing. Proteomic analysis of the same specimen suggested that matrix metalloproteinase 23 (MMP23) may play a key role in HBCS expediting the progress of wound healing. In this work, we aim to reveal the underlying mechanism of MMP23 in the dynamic process of cutaneous proliferation and repair period. In order to regulate the expression level of MMP23 in the local wound area, we leaded in adeno-associated virus (AAV) to specifically decreased expression quantity of MMP23 in rat skin. In contrast to the negative control groups, we found that the wound closed faster and the collagen fibers and neovascularization were significantly increased in AAV groups. These findings highlighted that MMP23 was involved in wound healing after traumatic injury, and managing the expression of MMP23 could be a potential intervention target to accelerate wound healing.

Antibacterial, Anti-Inflammatory, and Antioxidant Cotton-Based Wound Dressing Coated with Chitosan/Cyclodextrin–Quercetin Inclusion Complex Nanofibers

“…Inflammation is a vital part of wound healing, and its management can lead to its promotion. Immoderate inflammation can disrupt tissue regeneration and hinder wound healing . Both immune and regenerative cells can play roles in inducing an inflammatory response.…”

Exploring the Role of Dermal Sheath Cells in Wound Healing and Fibrosis

“…Heitman et al's study [38], which used P5 mice for transcriptome analysis, revealed that MMP11 and tissue inhibitor of metalloproteinase 1 (TIMP1) were expressed in anagen hair follicle DSCs, in contrast to MMP2, MMP19, and MMP27, which were expressed in DFs; MMP23 was found to be expressed in both cell types (Table 2). Recent research has shown that MMP23 is involved in inflammatory bowel disease [79] and wound healing after traumatic injury [80]. MMP11 was also found to be expressed in DSCs of human scalp [81].…”