β-Chitin Nanofibril Self-Assembly in Aqueous Environments

Montroni, Devis; Marzec, Bartosz; Valle, Francesco; Nudelman, Fabio; Falini, Giuseppe

doi:10.1021/acs.biomac.9b00481

Cited by 20 publications

(33 citation statements)

References 71 publications

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“…The obtained β-chitin nanofibers after 60 cycles had a mean diameter in the range of 10-15 nm. A similar nanofibril diameter (6.4-6.9 nm) was previously observed for β-chitin nanofiber prepared from Loligo vulgaris at different pH levels [36] and from Todarodes pacificus (5-10 nm) [5].…”

Section: Tem Analysissupporting

confidence: 82%

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Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas

et al. 2021

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β-chitin was isolated from marine waste, giant Humboldt squid Dosidicus gigas, and further converted to nanofibers by use of a collider machine under acidic conditions (pH 3). The FTIR, TGA, and NMR analysis confirmed the efficient extraction of β-chitin. The SEM, TEM, and XRD characterization results verified that β-chitin crystalline structure were maintained after mechanical treatment. The mean particle size of β-chitin nanofibers was in the range between 10 and 15 nm, according to the TEM analysis. In addition, the β-chitin nanofibers were converted into films by the simple solvent-casting and drying process at 60 °C. The obtained films had high lightness, which was evidenced by the CIELAB color test. Moreover, the films showed the medium swelling degree (250–290%) in aqueous solutions of different pH and good mechanical resistance in the range between 4 and 17 MPa, depending on film thickness. The results obtained in this work show that marine waste can be efficiently converted to biomaterial by use of mild extractive conditions and simple mechanical treatment, offering great potential for the future development of sustainable multifunctional materials for various industrial applications such as food packaging, agriculture, and/or wound dressing.

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Section: Tem Analysissupporting

confidence: 82%

“…This is only one example of their feasibility to be handled. This process takes advantage of chitin nanofibrils' natural tendency to self-assemble in aqueous media to form β-chitin fibers [36].…”

Section: Nanofibrous β-Chitin Film Preparation and Characterizationmentioning

confidence: 99%

Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas

et al. 2021

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“…Here, a single variable has been addressed, but future studies will focus on a multivariable analysis of this system. Chitin was chosen as the polymer to test this system as this biopolymer grants different advantages since it is cheap, mechanically robust [ 18 , 19 , 20 ], and has a propensity to self-assemble into crystalline nano-fibrils [ 21 , 22 , 23 ]. Moreover, the biocompatibility of this polymer promotes its use as material for medical applications, from drug delivery to wound healing and regenerative medicine [ 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Direct Ink Write Printing of Chitin-Based Gel Fibers with Customizable Fibril Alignment, Porosity, and Mechanical Properties for Biomedical Applications

Montroni

Kobayashi

Hao

et al. 2022

JFB

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A fine control over different dimensional scales is a challenging target for material science since it could grant control over many properties of the final material. In this study, we developed a multivariable additive manufacturing process, direct ink write printing, to control different architectural features from the nano- to the millimeter scale during extrusion. Chitin-based gel fibers with a water content of around 1500% were obtained extruding a polymeric solution of chitin into a counter solvent, water, inducing instant solidification of the material. A certain degree of fibrillar alignment was achieved basing on the shear stress induced by the nozzle. In this study we took into account a single variable, the nozzle’s internal diameter (NID). In fact, a positive correlation between NID, fibril alignment, and mechanical resistance was observed. A negative correlation with NID was observed with porosity, exposed surface, and lightly with water content. No correlation was observed with maximum elongation (~50%), and the scaffold’s excellent biocompatibility, which appeared unaltered. Overall, a single variable allowed a customization of different material features, which could be further tuned, adding control over other aspects of the synthetic process. Moreover, this manufacturing could be potentially applied to any polymer.

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“…The most important sources for the extraction of α-chitin, which is the most diffused form commercially available, are represented by the wastes of the seafood industry, constituted mainly by shells of crabs and shrimps. In recent years, the possible technological applications of β-chitin have been attracting growing interest − and have motivated its extraction from waste products. The richest source of β-chitin is squid pens.…”

Section: Introductionmentioning

confidence: 99%

Doxorubicin-Loaded Squid Pen Plaster: A Natural Drug Delivery System for Cancer Cells

Magnabosco

Ianiro

Stefani

et al. 2020

ACS Appl. Bio Mater.

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The native structure of the β-chitin in the gladius (squid pen) of Loligo vulgaris squid can be used as a natural plaster to entrap and release a model drug, doxorubicin, in a targeted and controlled way. Local pH determines the protonation state of the doxorubicin molecules, controlling the two phenomena. Confocal microscopy shows that doxorubicin is uniformly embedded in the βchitin squid pen and is not simply adsorbed on its surface. Coculture with HeLa cells reveals that the β-chitin squid pen plaster is perfectly biocompatible, while when it is loaded with doxorubicin it shows high cytotoxicity toward the cancer cells. The drug, once released, rapidly accumulates inside the cells. In conclusion, the native structure of a β-chitin squid pen can be potentially applied as a "green" pH-responsive drug vehicle for controlled release.

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β-Chitin Nanofibril Self-Assembly in Aqueous Environments

Cited by 20 publications

References 71 publications

Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas

Utilization of Marine Waste to Obtain β-Chitin Nanofibers and Films from Giant Humboldt Squid Dosidicus gigas

Direct Ink Write Printing of Chitin-Based Gel Fibers with Customizable Fibril Alignment, Porosity, and Mechanical Properties for Biomedical Applications

Doxorubicin-Loaded Squid Pen Plaster: A Natural Drug Delivery System for Cancer Cells

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