β-Catenin Signaling Promotes Proliferation of Progenitor Cells in the Adult Mouse Subventricular Zone

Adachi, Kazuhide; Mirzadeh, Zaman; Sakaguchi, Masanori; Yamashita, Toru; Nikolcheva, Tania; Gotoh, Yukiko; Peltz, Gary; Gong, Leyi; Kawase, Takeshi; Alvarez-Buylla, Arturo; Okano, Hideyuki; Sawamoto, Kazunobu

doi:10.1634/stemcells.2007-0177

Cited by 228 publications

(228 citation statements)

References 59 publications

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“…β-catenin pathway, also known as canonical Wnt pathway or β-catenin/TCF pathway, mediates cell proliferation by regulating cyclin D1 expression (Shtutman et al, 1999;Tetsu et al, 1999). In addition, recent studies including ours have shown that GSK-3β and β-catenin pathway are involved in the proliferation of neural precursor cells in both embryo and adult brains (Lie et al, 2005;Adachi et al, 2007;Hirsch et al, 2007;Boku et al, 2009). These results of studies suggest that bFGF might regulate proliferation of neural precursor cells through GSK-3β and β-catenin pathway.…”

Section: Introductionmentioning

confidence: 78%

“…β-catenin pathway is involved in regulating the proliferation of neural precursor cells both in embryo and adult brain (Lie et al, 2005;Adachi et al, 2007;Hirsch et al, 2007;Boku et al, 2009). Therefore, we hypothesized that ROCK regulates bFGF-induced proliferation of SH-SY5Y cells through β-catenin pathway.…”

Section: Consequently Sh-sy5y Cells May Fulfill the Definitions Of Nmentioning

confidence: 99%

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ROCK2 regulates bFGF-induced proliferation of SH-SY5Y cells through GSK-3β and β-catenin pathway

Boku¹,

Nakagawa²,

Toda³

et al. 2013

Brain Research

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Increased neurogenesis by promoting proliferation of neural precursor cells in the adult dentate gyrus might be beneficial for the treatment of psychiatric disorders. Results demonstrate that bFGF is necessary for the proliferation of neural precursor cells and that the glycogen synthase kinase-3β (GSK-3β) and β-catenin pathway plays a role in it. However, the detailed mechanism of proliferation of neural precursor cells remains unclear. To elucidate that mechanism, we investigated the role of Rho-associated coiled-coil kinase (ROCK) in bFGF-induced proliferation using SH-SY5Y cells as a model of neural precursor-like cells. Y27632, a specific inhibitor of ROCK, decreased bFGF-induced proliferation. Lithium (Li), an inhibitor of GSK-3β, recovered Y27632-decreased proliferation and quercetin (Que), an inhibitor of β-catenin pathway, reversed the recovery effect of Li. Both nuclear β-catenin and cyclin D1 expression were altered by bFGF, Y27632, Li, and Que in parallel with the case of proliferation. Furthermore, bFGF inactivated GSK-3β through increasing the phosphorylation of Ser 9 on GSK-3β, which is reversed by Y27632 through increased phosphorylation of Tyr 216 on GSK-3β. ROCK has two subtypes: ROCK1 and ROCK2. Investigation with siRNA for ROCKs showed that ROCK2 is involved in bFGF-induced proliferation, but not ROCK1. These results suggest that ROCK2 might mediate bFGF-induced proliferation of SH-SY5Y cells through GSK-3β and β-catenin pathway. Further investigation of detailed mechanisms regulating the ROCK2/GSK-3β/β-catenin pathway might engender the development of new therapeutic targets of psychiatric disorders.

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Section: Introductionmentioning

confidence: 78%

Section: Consequently Sh-sy5y Cells May Fulfill the Definitions Of Nmentioning

confidence: 99%

ROCK2 regulates bFGF-induced proliferation of SH-SY5Y cells through GSK-3β and β-catenin pathway

Boku¹,

Nakagawa²,

Toda³

et al. 2013

Brain Research

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“…Numerous studies have also reported roles for Wnt signaling in the SVZ. -catenin-responsive cells exist in the SVZ throughout the development of the CNS [140], and their activation promotes progenitor cell proliferation [141]. Activation of -catenin signaling increases the numbers of oligodendrocytes derived from this neurogenic site, while inhibition seems to reduce the glial cell number [142].…”

Section: Wnt Signaling and Adult Neurogenesis: Intracellular Componentsmentioning

confidence: 99%

Canonical and noncanonical Wnt signaling in neural stem/progenitor cells

Bengoa-Vergniory

Kypta

2015

Cell. Mol. Life Sci.

136

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“…26 The injection of a retrovirus carrying a constitutively active form of β-catenin or a highly selective GSK3β inhibitor, both of which activate Wnt/β-catenin signaling, increased the proliferation of transit-amplifying cells, which ultimately led to an increased number of new neurons in the OB. These results indicated that Wnt/β-catenin signaling positively regulates the proliferation of neuronal progenitors in the adult SVZ (Fig.…”

Section: Neural Stem Cells In the Subventricular Zonementioning

confidence: 99%

Mechanisms of Neurogenesis in the Normal and Injured Adult Brain

Sawada

Sawamoto

2013

Keio J. Med.

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Even in the adult brain, new neurons are continuously generated from endogenous neural stem cells that reside in two restricted regions: the subventricular zone (SVZ) of the lateral ventricle and the dentate gyrus of the hippocampus. These new neurons are integrated into the mature neuronal circuitry and become involved in various functions, thereby contributing to structural and functional plasticity in the adult brain. In this review, we summarize our recent findings on the regulatory mechanisms of SVZ neurogenesis under physiological and pathological conditions in various animal models. Some of these findings were presented in the Kitazato Prize Lecture at Keio University School of Medicine in

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β-Catenin Signaling Promotes Proliferation of Progenitor Cells in the Adult Mouse Subventricular Zone

Abstract: ABSTRACT

Cited by 228 publications

References 59 publications

ROCK2 regulates bFGF-induced proliferation of SH-SY5Y cells through GSK-3β and β-catenin pathway

ROCK2 regulates bFGF-induced proliferation of SH-SY5Y cells through GSK-3β and β-catenin pathway

Canonical and noncanonical Wnt signaling in neural stem/progenitor cells

Mechanisms of Neurogenesis in the Normal and Injured Adult Brain

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