Canonical and noncanonical Wnt signaling in neural stem/progenitor cells

Bengoa-Vergniory, Nora; Kypta, Robert M.

doi:10.1007/s00018-015-2028-6

Cited by 136 publications

(100 citation statements)

References 183 publications

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“…In the canonical Wnt signaling pathway, which has been better examined (7), β-catenin accumulates in the cytoplasm and eventually translocates to the nucleus where it acts as a transcriptional co-activator of transcription factors belonging to the T-cell factor/lymphoid enhancer factor-1(TCF/LEF) family. β-catenin that accumulates in the cytoplasm is degraded, however, by a protein complex that includes axin, glycogen synthase kinase-3 (GSK-3), protein phosphatase 2A (PP2A), casein kinase 1 (CK1) and adenomatous polyposis coli (APC) (6,8). This degradation complex becomes disrupted as soon as the Wnt protein binds to receptors of frizzled (Fz) and low-density lipoprotein receptor-related protein (LRP5/6), which activates multiple signaling cascades.…”

Section: Introductionmentioning

confidence: 99%

“…Wnt signaling regulates cell proliferation and differentiation, controls migration and patterning during embryonic development, and maintains tissue homeostasis in adults (4,5). Depending on the involvement of β-catenin, these signaling pathways have been classified as canonical (β-catenin-dependent) or non-canonical (β-catenin-independent) (6). In the canonical Wnt signaling pathway, which has been better examined (7), β-catenin accumulates in the cytoplasm and eventually translocates to the nucleus where it acts as a transcriptional co-activator of transcription factors belonging to the T-cell factor/lymphoid enhancer factor-1(TCF/LEF) family.…”

Section: Introductionmentioning

confidence: 99%

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IWP2 impairs the development of porcine somatic cell nuclear transfer embryos via Wnt signaling pathway inactivation

Huang

Yuan

et al. 2017

Biomedical Reports

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Abstract. Wnt signaling is critical in embryonic development and post-embryonic tissue homeostasis. The aim of the present study was to evaluate the expression levels of canonical Wnt signaling genes in porcine somatic cell nuclear transfer (SCNT) embryos. Quantitative polymerase chain reaction analysis was performed in porcine SCNT embryos, and the results indicated that the temporal expression patterns of canonical signaling genes were similar between in vivo and SCNT embryos from the 2-cell to the blastocyst stage. In addition, aberrant expression in a small number of Wnt signaling genes in SCNT embryos was identified. IWP2, an inhibitor of Wnt processing, was applied to the culture of SCNT embryos. The Wnt signaling pathway in the SCNT blastocysts may be inactivated via IWP2 treatment, reflecting the low expression levels of c-Myc and peroxisome proliferator-activated receptor δ. Furthermore, blastocyst hatching was damaged by IWP2 treatment. These findings indicate that the canonical Wnt signaling pathway is important for SCNT embryo development. IntroductionSomatic cell nuclear transfer (SCNT) is an effective method of reprogramming differentiated nuclei and has many potential applications (1). Owing to incomplete or faulty reprogramming, however, the cloning efficiency of SCNT embryos remains low (2,3). Aberrant reprogramming leads to changes in the expression levels of certain genes important for embryonic development. Studies on the expression of development-associated genes may be valuable for improving the developmental competence of SCNT embryos.Wnt signaling is a critical regulator of many cellular and physiological processes. Wnt signaling regulates cell proliferation and differentiation, controls migration and patterning during embryonic development, and maintains tissue homeostasis in adults (4,5). Depending on the involvement of β-catenin, these signaling pathways have been classified as canonical (β-catenin-dependent) or non-canonical (β-catenin-independent) (6). In the canonical Wnt signaling pathway, which has been better examined (7), β-catenin accumulates in the cytoplasm and eventually translocates to the nucleus where it acts as a transcriptional co-activator of transcription factors belonging to the T-cell factor/lymphoid enhancer factor-1(TCF/LEF) family. β-catenin that accumulates in the cytoplasm is degraded, however, by a protein complex that includes axin, glycogen synthase kinase-3 (GSK-3), protein phosphatase 2A (PP2A), casein kinase 1 (CK1) and adenomatous polyposis coli (APC) (6,8). This degradation complex becomes disrupted as soon as the Wnt protein binds to receptors of frizzled (Fz) and low-density lipoprotein receptor-related protein (LRP5/6), which activates multiple signaling cascades.Dysfunctions in Wnt signaling result in severe complications of embryonic development. For example, abnormalities were observed in the size and shape of mouse embryos lacking intact maternal/zygotic β-catenin during pre-and post-implantation development (5). By contrast, transient expression...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

IWP2 impairs the development of porcine somatic cell nuclear transfer embryos via Wnt signaling pathway inactivation

Huang

Yuan

et al. 2017

Biomedical Reports

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“…The Wnt family consists of 19 secreted glycolipoproteins, which bind to transmembrane receptors (Fzd) and signal through canonical and noncanonical pathways. Their roles in neural stem/progenitor cells are comprehensively documented in a recent review.. 12 Continuous production of new neurons in the SGZ of the dentate gyrus is involved in cognitive functions of learning, memory and aging, therefore a large body of research has been focused on adult hippocampal neurogenesis.…”

mentioning

confidence: 99%

Multiple roles of Ulk4 in neurogenesis and brain function

Liu

O’Brien

et al. 2017

Neurogenesis

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“…NSPCs are round neurospheres which able to self-renew and differentiate into neurons, astrocytes, and oligodendrocytes [42]. These cells are suitable for gene therapy, intracellular distribution of proteins, neurogenesis, tumorigenesis, and given rise to many interesting findings [42][43][44][45].…”

Section: Avian Neural Stem/progenitor Cellsmentioning

confidence: 99%

Concise Review: Avian Multipotent Stem Cells as a Novel Tool for Investigating Cell-Based Therapies

Farzaneh¹,

Farzaneh²,

Mozdziak³

2017

JDVAR

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The chick embryo is a favorite and great in vivo model, which has long been used in embryology, developmental biology and applied sciences. Also, chicken embryo is the accessible model for transplantation and tracking studies. Avian multipotent stem cells have the capability to self-renew and differentiate into a variety of cell types in vitro. Avian multipotent stem cells were successfully isolated from bone marrow (BM), metanephric tissue, lung, amniotic cells, cartilage, and other embryonic and adult tissues. Subsequent analysis was performed to evaluate these multipotent cells in vitro. Results of the present study strongly contribute to the information related to the ability of avian multipotent stem cells to differentiate into cells such as adipocytes, osteoblasts, chondrocytes, astrocytes, and other committed cells. Avian multipotent stem cells may provide a new source of avian-derived cell lines for basic research and potential therapeutic applications.

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Canonical and noncanonical Wnt signaling in neural stem/progenitor cells

Cited by 136 publications

References 183 publications

IWP2 impairs the development of porcine somatic cell nuclear transfer embryos via Wnt signaling pathway inactivation

IWP2 impairs the development of porcine somatic cell nuclear transfer embryos via Wnt signaling pathway inactivation

Multiple roles of Ulk4 in neurogenesis and brain function

Concise Review: Avian Multipotent Stem Cells as a Novel Tool for Investigating Cell-Based Therapies

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