β-Carotene stimulates browning of 3T3-L1 white adipocytes by enhancing thermogenesis via the β3-AR/p38 MAPK/SIRT signaling pathway

Mukherjee, Sulagna; Yun, Jong Won

doi:10.1016/j.phymed.2021.153857

Cited by 10 publications

(7 citation statements)

References 52 publications

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“…Moreover, in 3T3 cells, this carotenoid increased PGC1-α and carnitine palmitoyltransferase 1a (CPT1a), which are involved in thermogenesis. 69 These results corroborate those of Mukherjee and Yun (2022), that showed that 20 µM β-carotene upregulated not only phosphorylated AMPK ( pAMPK) and pACC, but also CPT1a and brown-specific genes (UCP1 and PGC-1α) in 3T3 cells after 6-8 days of treatment. 70 Five and 10 μM fucoxanthin increased lipolytic and AMPK activity and reduced lipid accumulation in mature adipocytes (3T3 cells).…”

Section: Carotenoids and Ampksupporting

confidence: 83%

“…69 These results corroborate those of Mukherjee and Yun (2022), that showed that 20 μM β-carotene upregulated not only phosphorylated AMPK (pAMPK) and pACC, but also CPT1a and brown-specific genes ( UCP1 and PGC-1α ) in 3T3 cells after 6–8 days of treatment. 70 Five and 10 μM fucoxanthin increased lipolytic and AMPK activity and reduced lipid accumulation in mature adipocytes (3T3 cells). 71 A concentration of 50 μg mL −1 fucoxanthin induced AMPK activation in 3T3 cells.…”

Section: Introductionmentioning

confidence: 99%

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Role of carotenoids in adipose tissue through the AMPK-mediated pathway

et al. 2023

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Section: Carotenoids and Ampksupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Role of carotenoids in adipose tissue through the AMPK-mediated pathway

et al. 2023

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“…A growing body of evidence indicates that phytochemicals mediate thermogenesis via the stimulation of the β3-AR [ 9 , 26 , 27 ]. These molecules target various pathways that are intricately linked to the process of adipogenesis, lipogenesis, lipolysis, and the fat oxidation process, all of which provide a fertile environment for high energy expenditure in the metabolic machinery [ 11 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Echinacoside Induces UCP1- and ATP-Dependent Thermogenesis in Beige Adipocytes via the Activation of Dopaminergic Receptors

Haddish

Yun

2023

J. Microbiol. Biotechnol.

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Echinacoside (ECH) is a naturally occurring phenylethanoid glycoside, isolated from Echinacea angustifolia , and this study aimed to analyze its effect on thermogenesis and its interaction with dopaminergic receptors 1 and 5 (DRD1 and DRD5) in 3T3-L1 white adipocytes and mice models. We employed RT-PCR, immunoblot, immunofluorescence, a staining method, and an assay kit to determine its impact. ECH showed a substantial increase in browning signals in vitro and a decrease in adipogenic signals in vivo. Additionally, analysis of the iWAT showed that the key genes involved in beiging, mitochondrial biogenesis, and ATP-dependent thermogenesis were upregulated while adipogenesis and lipogenesis genes were downregulated. OXPHOS complexes, Ca 2+ signaling proteins as well as intracellular Ca 2+ levels were also upregulated in 3T3-L1 adipocytes following ECH treatment. This was collectively explained by mechanistic studies which showed that ECH mediated the beiging process via the DRD1/5-cAMP-PKA and subsequent downstream molecules, whereas it co-mediated the α1-AR-signaling thermogenesis via the DRD1/5/SERCA2b/RyR2/CKmt pathway in 3T3-L1 adipocytes. Animal experiments revealed that there was a 12.28% reduction in body weight gain after the ECH treatment for six weeks. The effects of ECH treatment on adipose tissue can offer more insights into the treatment of obesity and metabolic syndrome.

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“…Beige adipocytes created due to fat browning are also enriched in the mitochondria and UCP1, such as BAT, and energy consumption increases due to cold and various stimuli [14]. In addition, numerous research studies are currently being actively conducted to prove the effectiveness of fat browning using ingredients derived from natural products [15][16][17][18][19]. Therefore, the promotion of WAT browning is receiving attention as a promising strategy for treating obesity by increasing energy consumption.…”

Section: Introductionmentioning

confidence: 99%

Hederagenin from Hedera helix promotes fat browning in 3T3-L1 adipocytes

Choi,

Lee,

Lim

et al. 2024

Preprint

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Background Currently, the regulation of energy homeostasis through increased energy consumption is attracting attention to treat obesity. White adipose tissue is known to play a role in accumulating energy by storing excess energy, while brown adipose tissue consumes energy and maintains body temperature. Thus, the browning of white adipose tissue has been confirmed to be effective in controlling obesity. Hedera helix (H. helix) has been widely used as a traditional medicine for various diseases. In several previous studies, hederagenin (HDG) from H. helix has demonstrated many biological activities. In this study, we investigated the anti-obesity effect of HDG on fat browning in 3T3-L1 adipocytes. Methods Cytotoxicity was assessed in 3T3-L1 cells through the Dimethyl sulfoxide and thiazolyl blue tetrazolium bromide (MTT) assay, while the extent of lipid accumulation was compared using the Oil red O (ORO) assay. Protein expression and mRNA expression were compared using Western blot and quantitative real-time polymerase chain reaction (qRT-PCR). Results Consequent to HDG treatment, a reduction in lipid accumulation was confirmed through oil red O staining. In addition, this study confirmed that HDG increases heat production by upregulating the expression of several targets related to thermogenesis, including uncoupling protein 1 (UCP1). This process is closely related to the inhibition of lipogenesis through the adenosine monophosphate-activated protein kinase (AMPK) phosphorylation signaling pathway and lipolysis through the protein kinase A (PKA) signaling pathway. Conclusions HDG is expected to be effective in promoting fat browning, indicating its potential as a natural anti-obesity candidate.

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β-Carotene stimulates browning of 3T3-L1 white adipocytes by enhancing thermogenesis via the β3-AR/p38 MAPK/SIRT signaling pathway

Cited by 10 publications

References 52 publications

Role of carotenoids in adipose tissue through the AMPK-mediated pathway

Role of carotenoids in adipose tissue through the AMPK-mediated pathway

Echinacoside Induces UCP1- and ATP-Dependent Thermogenesis in Beige Adipocytes via the Activation of Dopaminergic Receptors

Hederagenin from Hedera helix promotes fat browning in 3T3-L1 adipocytes

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