β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

Dabouian, Atiyeh; Bakhshi, Hadi; Irani, Shiva; Pezeshki‐Modaress, Mohamad

doi:10.1039/c7ra13237a

Cited by 35 publications

(19 citation statements)

References 43 publications

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“…Electrospinning may be utilized to fabricate nanofibrous scaffolds to imitate the natural extracellular matrix and also porous architecture structure to enhance the cellular infiltration (Pezeshki-Modaress et al 2015a , 2017 ). The electrospun poly(ε-caprolactone) (PCL) scaffold is one of the most widely used synthetic polymers due to its excellent biodegradability, biocompatibility, and mechanical properties (Dabouian et al 2018 ). Although PCL-based electrospun scaffold exhibits desirable characteristics, inherent hydrophobic nature of PCL limits the tissue engineering applications including cell adhesion, growth, and differentiation due to lack of adsorption of cell-adhesive proteins such as fibronectin, vitronectin, and collagen from the biological serum (Beachley and Wen 2010 ; Cipitria et al 2011 ; Pham et al 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Cold atmospheric plasma as a promising approach for gelatin immobilization on poly(ε-caprolactone) electrospun scaffolds

et al. 2019

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Poly(Ɛ-caprolactone) (PCL) is a biocompatible polymer with a high potential to be used in tissue engineering especially in tight tissues. In the current study, cold atmospheric plasma (CAP) is used as a promising method for immobilization of gelatin as a functional biomacromolecule on PCL nanofibrous substrates. The CAP surface modification leads to oxidation of chemical groups existing on the PCL surface without doing any damage to the bulk properties of biomaterials for gelatin biomacromolecule grafting. The water contact angle (WCA) of the CAP-treated surface and gelatin-grafted PCL using CAP indicates an effective increment in the hydrophilicity of the PCL surface. Also to achieve the highest levels of gelatin grafting on the PCL surface, two different grafting methods and gelatin concentration diversity are utilized in the grafting process. The immobilization of gelatin biomacromolecules onto the CAP surface-modified PCL nanofibers is investigated using scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). The gelatin-modified PCL substrates revealed uniform nanofibrous morphology with increased average fiber diameter. The results of FTIR spectra, including hydroxyl groups, NH groups, and amide II of gelatin-grafting peaks, confirm the gelatin immobilization on the surface of nanofibers. The metabolic activity of cultured mesenchymal stem cells (MSCs) on the surface-modified scaffolds is evaluated using MTT analysis ( P ≤ 0.05). The results of metabolic activity and also SEM and DAPI staining observations indicate proper attachment on the surface and viability for MSCs on the surface-immobilized nanofibrous scaffolds. Therefore, CAP treatment would be an effective method for biomacromolecule immobilization on nanofibers towards the enhancement of cell behavior.

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Section: Introductionmentioning

confidence: 99%

Cold atmospheric plasma as a promising approach for gelatin immobilization on poly(ε-caprolactone) electrospun scaffolds

et al. 2019

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“…According to studies, a weakness of PCL scaffold, which decreases the adhesion of cells on its surface, is the intrinsic hydrophobic property, and, consequently, lowering the cell-scaffold interaction [26] . One of the exciting solutions to this problem is to combine this scaffold with another natural polymer Here, CAP treatment on the PCL surface was used to graft CMC.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber

Shapourzadeh

Atyabi

Irani

et al. 2020

ibj

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Background: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. Methods: In this work, CMC (20%) was grafted to PCL nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface. Results: The results of FTIR confirmed CMC (20%) graft on PCL scaffold. The MTT assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of ADSCs to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions. Conclusion: Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.

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“…Despite these excellent properties, PCL due to insufficient biological recognition sites and poor intrinsic hydrophobicity has some drowpoints in bio applications such as desired cell adhesion . A number of works are published on electrospinning of PCL in different solvent systems in the combination with relatively hydrophilic polymers such as chitosan, poly(vinyl alcohol) (PVA), collagen, silk, and gelatin (GEL) . GEL drives from collagen possesses biological activities and makes it suitable for using as a main component of wound dressings and scaffolds in medical applications .…”

Section: Introductionmentioning

confidence: 99%

“…11,12 A number of works are published on electrospinning of PCL in different solvent systems in the combination with relatively hydrophilic polymers such as chitosan, poly(vinyl alcohol) (PVA), collagen, silk, and gelatin (GEL). 13,14 GEL drives from collagen possesses biological activities and makes it suitable for using as a main component of wound dressings and scaffolds in medical applications. 15,16 Many reports show that GEL derivatives have an excellent potential to manage the adhesion, migration, growth and organization of the cells during wound healing process.…”

Section: Introductionmentioning

confidence: 99%

Biphasic, tough composite core/shell PCL/PVA‐GEL nanofibers for biomedical application

Akbarzadeh

Pezeshki‐Modaress

Zandi

2019

J of Applied Polymer Sci

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Emulsion electrospinning using natural and synthetic polymers, including two dissimilar materials is a promising technique for nanofibers fabrication in a core/shell configuration for tissue engineering, controlled or sustained drug delivery and dressing applications. In this study, we designed and fabricated core/shell nanofibers based on polycaprolactone (PCL) as core material and poly(vinyl alcohol) (PVA)‐gelatin (GEL) blend as shell materials (PCL/PVA‐GEL) to achieve high mechanical properties, good cell growth, and proliferation via emulsion electrospinning. The effect of water to acetic acid ratio in the solvent system (8:2, 7:3, 6:4, 5:5) and also type and concentration (3, 5, 7 w/v %) of surfactant on emulsion stability and nanofibers morphology were investigated. The emulsion containing 2% Tween80 and 1% Span60 as surfactants were selected by considering the stability of emulsion and uniform fiber morphology. In the tensile strength and elongation at break, 53 and 8% increase in the crosslinked wet state of the PCL/PVA‐GEL nanofibers compared with PVA‐GEL nanofibers were observed respectively. The cell culture results indicated that the PCL/PVA‐GEL nanofibers surface has presented suitable interaction with fibroblast cells and cells attached and proliferated well on the fabricated substrate within 24 and 48 hours and also would be a good candidate for biomedical applications. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48713.

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β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

Abstract: Electrospun PCL scaffolds containing β-carotene as a natural osteogenic material can differentiate MSCs to osteoblasts without using external differential agents.

Cited by 35 publications

References 43 publications

Cold atmospheric plasma as a promising approach for gelatin immobilization on poly(ε-caprolactone) electrospun scaffolds

Cold atmospheric plasma as a promising approach for gelatin immobilization on poly(ε-caprolactone) electrospun scaffolds

Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber

Biphasic, tough composite core/shell PCL/PVA‐GEL nanofibers for biomedical application

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