α2-Adrenoceptors in the guinea-pig uterus: heterogeneity in the circular and longitudinal smooth muscle layers

Haynes, John M.; Adams, Glen D.; Pennefather, Jocelyn N.

doi:10.1016/0014-2999(93)90386-v

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…With reference to the participation of NA in the contractility of healthy uteri, it is known that in various species this neurotransmitter stimulates (via α-ARs) and inhibit (via β-ARs) myometrial contractile activity 18 – 21 . Moreover, the role of β2- and β3-ARs in relaxation of human 22 , 23 , rat 24 , 25 and pig 26 , 27 MYO was indicated.…”

Section: Introductionmentioning

confidence: 99%

Role of beta-adrenergic receptor subtypes in pig uterus contractility with inflammation

Jana

Całka

2021

Sci Rep

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Uterine inflammation is a very common and serious condition in domestic animals. To development and progression of this pathology often lead disturbances in myometrial contractility. Participation of β1-, β2- and β3-adrenergic receptors (ARs) in noradrenaline (NA)-influenced contractility of the pig inflamed uterus was studied. The gilts of SAL- and E.coli-treated groups were administered saline or E.coli suspension into the uterine horns, respectively. Laparotomy was only done in the CON group. Compared to the period before NA administration, this neurotransmitter reduced the tension, amplitude and frequency in uterine strips of the CON and SAL groups. In the E.coli group, NA decreased the amplitude and frequency, and these parameters were lower than in other groups. In the CON, SAL and E.coli groups, β1- and β3-ARs antagonists in more cases did not significantly change and partly eliminated NA inhibitory effect on amplitude and frequency, as compared to NA action alone. In turn, β2-ARs antagonist completely abolished NA relaxatory effect on these parameters in three groups. Summarizing, NA decreases the contractile amplitude and frequency of pig inflamed uterus via all β-ARs subtypes, however, β2-ARs have the greatest importance. Given this, pharmacological modulation of particular β-ARs subtypes can be used to increase inflamed uterus contractility.

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Section: Introductionmentioning

confidence: 99%

Role of beta-adrenergic receptor subtypes in pig uterus contractility with inflammation

Jana

Całka

2021

Sci Rep

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“…However, other studies have demonstrated that a2-adrenoceptor stimulation or adenylate cyclase activation can inhibit the phenylephrine-stimulated accumulation of [3H]-inositol phosphates (Manopoulos et al, 1991;Haynes et al, 1993).…”

Section: Statisticsmentioning

confidence: 99%

α‐Adrenoceptor mediated responses of the cauda epididymis of the guinea‐pig

Haynes

Hill

1996

British J Pharmacology

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1 The subtypes of Lx-adrenoceptor mediating the contractile responses of the cauda epididymis of the guinea-pig were investigated. The ocx-adrenoceptor agonist phenylephrine, but not the LX2-adrenoceptor agonist, xylazine (up to 10 gM), elicited concentration-dependent contractions from preparations of cauda epididymis (EC50 3.4 gM). The L-type Ca2+ channel antagonist, nifedipine (10 gM), reduced the maximal response to phenylephrine (by 77%). Preincubation of tissues with the alB-adrenoceptoralkylating agent, chloroethylclonidine (50 juM, 30 min), shifted phenylephrine concentration-response curves to the right (4 fold) only when the a2-adrenoceptor antagonist idazoxan (100 nM) was included during the pre-incubation with chloroethylclonidine. 2 Xylazine (1 /IM) significantly shifted phenylephrine concentration-response curves to the left (3 fold); this effect was attenuated by idazoxan (100 nM). Both the incubation of preparations with nifedipine (10 gM) and the pre-incubation of preparations with chloroethylclonidine (50 gM, 30 min) attenuated the potentiating effects of xylazine (1 gM). Protection of a2-adrenoceptors with idazoxan (100 nM) during the chloroethylclonidine (50 gM, 30 min) incubation restored the xylazine-mediated enhancement of phenylephrine concentration-response curves. Pertussis toxin (200 ng ml-1, 24 h) attenuated the xylazine (1 gM)-mediated potentiation of phenylephrine concentration-response curves.3 Following the pre-incubation of preparations with chloroethylclonidine (50 gM, 30 min) 5-methylurapidil (10 nM to 3 gM) shifted phenylephrine concentration-response curves, in parallel, to the right with mean pKB values in the range of 8.27 (at 10 nM 5-methylurapidil) to 7.76 (at 3 gM 5-methylurapidil), the addition of idazoxan (100 nM) to the incubation medium did not significantly affect the 5-methylurapidil (10 to 300 nM) pKB values (8.41 to 7.64, respectively). In the presence of both idazoxan (100 nM) and nifedipine (10 pM), and following the pre-incubation with chloroethylclonidine (50 gM, 30 min), 5-methylurapidil (30 to 300 nM) still shifted phenylephrine concentration-response curves to the right (pKB values 7.77 to 7.36, respectively).

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Expression of alpha and beta adrenergic receptors in the pig uterus during inflammation

et al. 2018

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α2-Adrenoceptors in the guinea-pig uterus: heterogeneity in the circular and longitudinal smooth muscle layers

Cited by 6 publications

References 23 publications

Role of beta-adrenergic receptor subtypes in pig uterus contractility with inflammation

Role of beta-adrenergic receptor subtypes in pig uterus contractility with inflammation

α‐Adrenoceptor mediated responses of the cauda epididymis of the guinea‐pig

Expression of alpha and beta adrenergic receptors in the pig uterus during inflammation

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