Expression of alpha and beta adrenergic receptors in the pig uterus during inflammation

Meller, K.A.; Całka, Jarosław; Kaczmarek, Monika M.; Jana, Barbara

doi:10.1016/j.theriogenology.2018.06.025

Cited by 19 publications

(44 citation statements)

References 48 publications

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“…In the experiment, the expression patterns of M2R, M3R, and α-7 nAChR in the inflamed uteri were examined. The findings of macroscopic and histological studies of the tissue used in the research were published previously and noted severe endometritis with oedema and hyperaemia, an increased number of neutrophils, and damage to glands and/or luminal epithelium in the E. coli-administered uteri (23).…”

Section: Discussionmentioning

confidence: 82%

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Escherichia coli -induced inflammation changes the expression of acetylcholine receptors (M2R, M3R, and α-7 nAChR) in the pig uterus

Jana

Całka

Palus

et al. 2020

Journal of Veterinary Research

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IntroductionThe influence of inflammation on the patterns of muscarinic 2 and 3 receptor subtypes (M2R and M3R), and α-7 nicotinic acetylcholine receptor (α-7 nAChR) expression in the porcine uterus was investigated.Material and MethodsOn day three of the oestrous cycle of gilts aged 7–8 months with body weight 90–120 kg, either an E. coli suspension (E. coli group, n = 5) or saline (Sal group, n = 5) was administered into the uterine horns via laparotomy or only laparotomy was performed on control swine (Ctrl group, n = 5). After eight days, and the onset of severe acute endometritis in the E. coli group, the uterine mRNA and protein receptor expression levels were determined using real-time RT-PCR and Western blotting, respectively, with receptor localisation by immunofluorescence.ResultsThe studied receptors were in the luminal epithelium, glands, blood vessels, and myometrial muscle cells of all gilts. The M2R mRNA level was lower in the inflamed endometrium compared to the Ctrl and Sal groups. Also in this tissue, the expression of M3R mRNA and protein was lower than in the Ctrl and Sal groups. The M3R protein level in the bacterially challenged myometrium was found to be increased compared to unadministered groups. In the endometrium of the E. coli group, the α-7 nAChR protein level was lower than in the Sal group, and in the myometrium it was reduced in relation to both the other groups. P values were ≤ 0.05 in all cases.ConclusionInflammation causes alterations in the M2R, M3R, and α-7 nAChR expression in the pig uterus, suggesting their significance in the course and repercussions of uterine inflammation.

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Section: Discussionmentioning

confidence: 82%

“…For immunofluorescent staining, tissues were cryoprotected in 18% sucrose until sectioning, and for histopathological assessment they were embedded in paraffin in the standard manner. The findings of the macroscopic and histological studies of the uteri were published previously (23).…”

Section: Animalsmentioning

confidence: 99%

Escherichia coli -induced inflammation changes the expression of acetylcholine receptors (M2R, M3R, and α-7 nAChR) in the pig uterus

Jana

Całka

Palus

et al. 2020

Journal of Veterinary Research

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“…Several previous studies have reported on the biphasic responses of AC or cAMP to α2-AR agonism during inflammation [24,26,[50][51][52]. The findings indicated that α2-AR has inhibitory and stimulatory components.…”

Section: Discussionmentioning

confidence: 85%

Dexmedetomidine Preconditioning Protects Rats from Renal Ischemia–Reperfusion Injury Accompanied with Biphasic Changes of Nuclear Factor-Kappa B Signaling

Bao

Tang

Wang

2020

Journal of Immunology Research

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Acute kidney injury (AKI) is one of the most common and troublesome perioperative complications. Dexmedetomidine (DEX) is a potent α2-adrenoceptor (α2-AR) agonist with anti-inflammatory and renoprotective effects. In this study, a rat renal ischemia–reperfusion injury (IRI) model was induced. At 24 h after reperfusion, the IRI-induced damage and the renoprotection of DEX preconditioning were confirmed both biochemically and histologically. Changes in nuclear factor-kappa B (NF-κB), as well as its downstream anti-inflammatory factor A20 and proinflammatory factor tumor necrosis factor-α (TNF-α), were detected. Atipamezole, a nonselective antagonist, was then added 5 min before the administration of DEX to further analyze DEX’s effects on NF-κB, and another anti-inflammatory medicine, methylprednisolone, was used in comparison with DEX, to further analyze DEX’s effects on NF-κB. Different concentrations of DEX (0 nM, 0.1 nM, 1 nM, 10 nM, 100 nM, 1 μM, and 10 μM) were applied to preincubated human renal tubular epithelial cell line (HK-2) cells in vitro. After anoxia and reoxygenation, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium assay and enzyme-linked immunosorbent assay (ELISA) were performed to evaluate the levels of NF-κB downstream anti-inflammatory cytokines. The results showed that, unlike methylprednisolone, DEX preconditioning led to a time-dependent biphasic change (first activation then inhibition) of NF-κB in the rat renal IRI models that were given 25 μg/kg i.p. It was accompanied by a similarly biphasic change of TNF-α and an early and persistent upregulation of A20. In vitro, DEX’s cellular protection showed a concentration-dependent biphasic change which was protective within the range of 0 to 100 nM but became opposite when concentrations are greater than 1 μM. The changes in the A20 and NF-κB messenger RNA (mRNA) levels were consistent with the renoprotective ability of DEX. In other words, DEX preconditioning protected the rats from renal IRI via regulation biphasic change of NF-κB signaling.

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“…To perform immunofluorescent staining, tissues were cryoprotected in 18% sucrose until sectioning, while for the histopathological examination they were routinely embedded in paraffin. The findings of macroscopic and histological assessment of uteri were presented previously [33]. The uterine horns fragments from the middle part, were placed on ice and transported to the laboratory within 5 min after collection, for study of the contractility.…”

Section: Methodsmentioning

confidence: 99%

“…NPY and its receptors (Y1Rs, Y2Rs) are involved in inflammatory processes of intestines [30], chronic pain [31] as well as in cardiovascular disease pathogenesis [11]. To date, only the contribution of main autonomic neurotransmitters-noradrenaline (NA) and acetylcholine (ACh) in the contractility of the inflamed uterus [6,9,32] and the expression of noradrenergic receptors in pathologically-changed organ [33] were evaluated. In view of these data, it is hypothesized that uterine inflammation changes the expression of YRs and that it conditions the NPY effect on the contractility of an inflamed uterus.…”

Section: Introductionmentioning

confidence: 99%

Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility

2020

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In the experiment the influence of inflammation on neuropeptide Y (NPY) receptor subtype 1 (Y1Rs) and 2 (Y2Rs) expression pattern in the gilt myometrium and NPY effect alone or with Y1Rs and Y2Rs antagonists on contractility of an inflamed uterus were recognized. On Day 3 of the estrous cycle, either E.coli suspension (E.coli group) or saline (SAL group) were administered into uterine horns. In the control gilts (CON group), only laparotomy was carried out. Eight days later, E.coli treatment evoked severe acute endometritis, significantly reduced Y1Rs mRNA and protein expression and increased Y2Rs protein expression in myometrium in relation to the CON and SAL groups. Compared to period before NPY application, NPY (10 −7 M) significantly reduced amplitude in myometrium and endometrium/myometrium and frequency in myometrium of the CON and SAL groups and amplitude in endometrium/myometrium and frequency in strips of the E.coli group. In this group, after using NPY (10 −7 M), the amplitude rose significantly in both kinds of strips, while frequency fell in endometrium/myometrium in relation to the SAL group. In the CON and SAL groups, NPY (10 −7 M) with Y1Rs antagonist significantly increased the amplitude in endometrium/ myometrium, while with Y2Rs antagonist caused a rise in myometrium. In the E.coli group after using Y1Rs or Y2Rs antagonist and NPY (10 −7 M) amplitude did not significantly change in endometrium/myometrium, and this parameter was significantly lower after using the NPY and Y1Rs antagonist than in other groups. Y1Rs antagonist and NPY (10 −8 , 10 −7 M) significantly increased frequency in endometrium/myometrium of the CON and SAL groups. In the E.coli group, Y1Rs or Y2Rs antagonists and NPY (10 −7 M) significantly increased frequency in myometrium and it was significantly higher than in the CON group. Inflammation changes Y1Rs and Y2Rs expression in myometrium of the inflamed pig uterus and NPY reduces this organ contractility by Y1Rs and Y2Rs.

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Expression of alpha and beta adrenergic receptors in the pig uterus during inflammation

Cited by 19 publications

References 48 publications

Escherichia coli -induced inflammation changes the expression of acetylcholine receptors (M2R, M3R, and α-7 nAChR) in the pig uterus

Escherichia coli -induced inflammation changes the expression of acetylcholine receptors (M2R, M3R, and α-7 nAChR) in the pig uterus

Dexmedetomidine Preconditioning Protects Rats from Renal Ischemia–Reperfusion Injury Accompanied with Biphasic Changes of Nuclear Factor-Kappa B Signaling

Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility

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