α-Pinene, Caryophyllene and β-Myrcene from Peucedanum terebinthaceum Essential oil: Insecticidal and Repellent Effects on Three Stored-Product Insects

Abstract

The aim of this work was to evaluate the bioactivities of Peucedanum terebinthaceum (Fisch.) Fisch. ex Turcz. essential oil and its three second rich constituents against Tribolium castaneum Herbst, Lasioderma serricorne Fabricius and Liposcelis bostrychophila Badonnel. The essential oil from aerial part of P. terebinthaceum was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. Thirty-three constituents were confirmed by GC-MS, which accounting for 91.5% of the total oil. The principal constituents included β-thujene (21.4%), β-terpinene (11.8%), germacrene D (9.4%) and dihydro-cis-α-copaene-8-ol (8.0%). Besides, β-myrcene (6.2%), Linalyl isovalerate (4.3%), α-pinene (4.0%), caryophyllene (3.6%), (Z)α-farnesene (3.6%) and β-elemene (3.0%) were also detected in relatively lower content. The essential oil possessed promising potential in pest control, as it showed strong contact toxicity and repellent effects on T. castaneum and L. serricorne. Three major constituents α-pinene, caryophyllene and β-myrcene were toxic to three insect species in contact assays and showed repellent effects on T. castaneum and L. bostrychophila. This work revealed the insecticidal capacity of P. terebinthaceum and would provide some information for the development of new strategies in the control of insect pests.

Chemical Composition and Spectrum of Insecticidal Activity of the Essential Oils of Ocimum gratissimum L. and Cymbopogon citratus Stapf on the Main Insects of the Cotton Entomofauna in Côte d'Ivoire

“…These findings were also confirmed by our results since the main compounds of O. gratissimum and C. citratus essential oils were found to be monoterpenes. The results were also supported different monoterpenese such as myrcene, [29] neral, geranial, p‐ cymene [30,31] and thymol [32] . However, the insecticidal activity of the essential oils could also be explained by the presence of minor compounds including geraniol, 6‐methyl‐5‐heptene‐2‐one for that of C. citratus and α‐thujene, myrcene, β‐caryophyllene and β‐Selinene for O. gratissimum .…”

Pesticidal Activity and Mode of Action of Monoterpenes

“…Myrcene. Myrcene is present in many plant species, such as Cannabis sativa, 56 Peucedanum terebinthaceum, 57 Artemisia campestris, 58 Siparuna guianensis, 59 and C. sinensis. 60 Laboratory screening bioassays show that myrcene exhibits insecticidal activities against sanitary and stored product insects and antimicrobial activities against pathogenic bacteria.…”

“…For example, it shows fumigant toxicity against two ectoparasites, Dermanyssus gallinae (LC 50 , 78 mg/L) and Hyalomma dromedarii (LC 50 , 92 mg/L) 56 , and three grain pest insects, Tribolium confusum, C. maculatus, and Sitophilus oryzae. 60 Myrcene is also toxic to the larvae of the mosquito Culex quinquefasciatus 58,61 and the adults of the housefly Musca domestica, 58,61 A. aegypti, 19 and A. albopictus, 19 and it has repellent effects on T. castaneum (LC 50 , 22 mg/L air), 57 cigarette beetle (Lasioderma serricorne), 57 Liposcelis bostrychophila, 57 and tobacco aphid (Myzus persicae). 59 Myrcene has 45 oral or contact, adults (larvae) of Aedes aegypti and Aedes albopictus 19 Pimpinella anisum 46 fumigant, Callosobrunchus maculatus and Sitophilus zeamais 45 Eugenia uniflora 47 feeding deterrent, Tribolium castaneum 46 Lippia multiflora 48 topical and fumigant, Cimex lectularius 50 Ocimum basilicum 53 fumigant, Drosophila melanogaster 47 fumigant, Bemisia tabaci 48 fumigant, Thrips palmi 53 limonene Seriphidium brevifolium 70 larvicidal, Aedes albopictus 70 Launaea taraxacifolia 73 contact, Sitophilus oryzae 73 Citrus sinensis 45,60 contact, larvae (eggs) of Haematobia irritans 74 Pimpinella anisum 46 fumigant, Callosobrunchus maculatus and Sitophilus zeamais 45 Peumus boldus 71 feeding deterrent, Tribolium castaneum 46 Rosmarinus officinalis 69 oral or contact, larvae of Culex quinquefasciatus low antibacterial activity against the plant pathogenic bacteria A. tumefaciens and E. carotovora.…”

“…α-Pinene can be obtained from many plant species, such as Cannabis sativa, 56 Peucedanum terebintha-ceum, 57 and Artemisia campestris. 58 It has fumigation activity against various pest ticks such as Hyalomma dromedarii, 56 stored product insects such as Liposcelis bostrychophila (LC 50 , 1.4 mg/L air), 57 Sitophilus oryzae, 60 and C. maculatus, 103 sanitary insects such as C. quinquefasciatus 15 and Drosophila melanogaster, 47 and vegetable and fruit insect pests such as Thrips palmi 53 in laboratory screening bioassays. In addition, α-pinene is larvicidal, 58 adulticidal, 61 feeding deterrent, 86 antibacterial, 75 herbicidal, 104 and nematicidal.…”

“…Dermanyssus gallinae and Hyalomma dromedarii56 Peucedanum terebinthaceum57 contact and repellent, Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila57 …”