α-lipoic acid inhibits oxidative stress in testis and attenuates testicular toxicity in rats exposed to carbimazole during embryonic period

Prathima, Papisetty; Venkaiah, Kaduru; Pavani, Ramanadhapuram; Thathapudi, Daveedu; Munikumar, Manne; Gobinath, M.; Valli, M.; Sainath, S.B.

doi:10.1016/j.toxrep.2017.06.009

Cited by 38 publications

(22 citation statements)

References 57 publications

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“…The pharmacologic activity of ALA has been widely studied due to its important antioxidant function in animals and humans. In rats, ALA has been found to attenuate testicular injury induced by exposure to various toxins by inhibiting oxidative stress in the testes [24,25]. In the current study, HS was found to cause histological changes in testicular tissue, as evidenced by hematoxylin and eosin staining.…”

Section: Discussionsupporting

confidence: 52%

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

Xiong

Yin

et al. 2020

Animals

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Heat stress (HS) causes testicular injury, resulting in decreased fertility. Alpha-lipoic acid (ALA) is a well-known antioxidant. The aim of this study was to investigate the protective effects of ALA on HS-induced testicular damage in chickens. Histological changes; biomarkers of oxidative stress, including glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA); markers of endoplasmic reticulum (ER) stress, including glucose-regulated protein 78 (GRP78) and CCAAT/enhancer binding protein homologous protein (CHOP); apoptosis-related modulators, including Bax, Bcl-2, and caspase 3, in testicular tissue and serum testosterone levels were evaluated in chickens under heat stress. Heat stress induces spermatogenic cell abnormalities in chicken testes. Compared to the HS group, the histomorphological abnormalities in testicular tissue were visibly ameliorated, with significant increases in the enzyme activities of GPx, SOD, and CAT, increased serum testosterone concentration, and decreased MDA levels in the ALA + HS group. Consistent with these results, compared with the HS group, the protein levels of GRP78, CHOP, caspase 3, and Bax were significantly decreased, whereas Bcl-2, StAR, and 3β-HSD protein levels were increased in the ALA + HS group. Collectively, these findings suggest that ALA significantly ameliorates the heat-induced histomorphological abnormalities in the testes and decreased testosterone production by potentiating the activities of anti-oxidative enzymes (GPx, SOD, and CAT), inhibiting ER stress-related apoptotic pathways (Bax, Bcl-2, and caspase 3), and increasing steroidogenic gene (StAR and 3β-HSD) expression in chickens.

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Section: Discussionsupporting

confidence: 52%

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

Xiong

Yin

et al. 2020

Animals

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“…In this regard, Shen, Jiang, Li, Hu, and Li () showed that ALA can increase cryo‐protective capacity for frozen‐thawed boar spermatozoa in regard to sperm motility, enzymatic antioxidants and pregnancy rate (Shen et al, ). This group also showed that ALA could recover reproductive health through its antioxidant and steroidogenic properties in male rats exposed to carbimazole (Prathima et al, ).…”

Section: Discussionmentioning

confidence: 97%

Alpha‐lipoic acid minimises reactive oxygen species‐induced damages during sperm processing

et al. 2019

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Shearing forces during sperm preparation for assisted reproduction techniques may lead to excessive production of reactive oxygen species (ROS) which may have an unpleasant effect on embryonic development. In the current study, we assessed the effect of alpha‐lipoic acid (ALA) on ROS‐induced damages during sperm preparation process. Semen samples were collected from 15 normozoospermic men. Each semen sample was divided into two parts; one part was washed and centrifuged with sperm washing medium plus 0.02 mM ALA. Then, sperm pellet was diluted and incubated for 1 hr at 37°C in sperm washing media in the absence (ALA−) or presence of 0.02 mM ALA (ALA+). The second part was washed and centrifuged with sperm washing media in the absence of ALA, and then, sperm pellet was incubated for 1 hr at 37°C in sperm washing media in the absence (ALA−) or presence of 0.02 mM ALA (ALA+). Sperm viability, motility, intracellular oxidative stress and DNA fragmentation were assessed by eosin‐nigrosin, computer‐assisted sperm analysis system, H2DCFDA staining and acridine orange staining respectively. Our results showed that addition of ALA as a fat‐ and water‐soluble antioxidant to sperm washing media maintains sperm viability and motility by reduction in ROS production and can also protect sperm DNA integrity.

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“…They explained this effect due to the lipophilic nature ALA which can easily cross cell membranes and effectively remove the free radicals. [ 42 ]…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the possible protective effect of alpha lipoic acid on testicular toxicity induced by polychlorinated biphenyl in adult albino rats: A histological study

Elbakary

Tawfik

Amer

2020

J Microsc Ultrastruct

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Introduction: Polychlorinated biphenyl (PCB) is considered one of the environmental pollutants. It is used as hydraulic coils in vacuum pumps, pesticides transformers, heat-exchange systems, capacitors and as additives in adhesive inks, paints, plastics, copying paper and sealants. Alpha lipoic acid (ALA) is an antioxidant substance normally present in mitochondria as a coenzyme. Aim of the Work: To evaluate the protective effect of ALA on PCB induced testicular toxicity. Materials and Methods: Twenty five adult male albino rats were used in this study. They were divided into four groups, a control group included 10 rats, group II rats received alpha lipoic acid 25mg/Kg /day orally for 30 days, group III rats received PCB 5mg /Kg/day orally for 30 days and group IV rats received both PCB and alpha lipoic acid at the same previous dose for 30 days. At the appropriate time, the specimens were taken and prepared for light and electron microscope study. Results: LM examination revealed structural alterations in group III in the form of wide spaces between seminiferous tubules that contain homogeneous acidophilic substance, partial or complete detachment of the tubules from the basement membrane and total distorted irregular shaped tubules. Also dilated congested blood vessels were seen. EM examination of this group revealed Sertoli cells with cytoplasmic vacuolation and dilated rER. The basement membrane appeared as thick and irregular line under Sertoli and spermatogenic cells and it was interrupted in some points. Primary spermatocyte appeared shrunken while others revealed vacuoles in the cytoplasm and perinuclear dilatation. Leydig cells showed irregular vacuoles and swollen destroyed mitochondria. Amelioration of the previous histological changes could be detected in group IV. Conclusion: It could be concluded that alpha-lipoic acid has a protective effect against PCB induced testicular toxicity.

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α-lipoic acid inhibits oxidative stress in testis and attenuates testicular toxicity in rats exposed to carbimazole during embryonic period

Abstract: Graphical abstract

Cited by 38 publications

References 57 publications

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

Alpha‐lipoic acid minimises reactive oxygen species‐induced damages during sperm processing

Evaluation of the possible protective effect of alpha lipoic acid on testicular toxicity induced by polychlorinated biphenyl in adult albino rats: A histological study

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