Alpha‐lipoic acid minimises reactive oxygen species‐induced damages during sperm processing

Abstract

Shearing forces during sperm preparation for assisted reproduction techniques may lead to excessive production of reactive oxygen species (ROS) which may have an unpleasant effect on embryonic development. In the current study, we assessed the effect of alpha‐lipoic acid (ALA) on ROS‐induced damages during sperm preparation process. Semen samples were collected from 15 normozoospermic men. Each semen sample was divided into two parts; one part was washed and centrifuged with sperm washing medium plus 0.02 mM ALA. Then, sperm pellet was diluted and incubated for 1 hr at 37°C in sperm washing media in the absence (ALA−) or presence of 0.02 mM ALA (ALA+). The second part was washed and centrifuged with sperm washing media in the absence of ALA, and then, sperm pellet was incubated for 1 hr at 37°C in sperm washing media in the absence (ALA−) or presence of 0.02 mM ALA (ALA+). Sperm viability, motility, intracellular oxidative stress and DNA fragmentation were assessed by eosin‐nigrosin, computer‐assisted sperm analysis system, H2DCFDA staining and acridine orange staining respectively. Our results showed that addition of ALA as a fat‐ and water‐soluble antioxidant to sperm washing media maintains sperm viability and motility by reduction in ROS production and can also protect sperm DNA integrity.

Randomized clinical trial of combined therapy with oral α‐lipoic acid and NB‐UVB for nonsegmental stable vitiligo

“…Furthermore, the combination therapy maintained the membrane lipid ratio and reduced oxidative stress level. 13 Another study showed an antioxidant complex, containing the Comparing to other antioxidants, ALA showed stronger antioxidative activities, owing to its lipid-and water-soluble characteristics 15 and the ability to regenerate vitamins C and E. 16 We were interested in whether ALA as a sole antioxidant can increase the efficacy of NB-UVB in vitiligo. In our previous study, we evaluated the triple-combination treatment with ALA, NB-UVB and betamethasone injection on progressive nonsegmental vitiligo patients.…”

Role of alpha lipoic acid in protecting testes of adult rats from lead toxicity

“…Whereas, unbalanced in oxidant/antioxidant status with the increase formation of LPO (35) associated with destruction of sperm plasma membrane, losing integrity of mitochondrial membrane and sperm DNA damage leading to infertility (34). Co-treatment of lead with ALA shows a significant improvement in sperm parameters may be due the potential antioxidant properties of ALA through its ability to scavenge FRs in aqueous and non-aqueous phases (36) and its ability to synthesized robust shield surrounding the sperm cell membrane (23). In our study, histopathological alterations in lead treated rats characterized with degenerative changes was observed in lead acetate treated rats due to negatively effects on male reproductive system.…”

Aqueous leaf extract ofMoringa oleiferareduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro

“…Also, alpha-lipoic acid, an organo-sulphur compound derived from octanoic acid, is regarded as a universal antioxidant as it acts as a cofactor for many enzymes and is shown to restore endogenous antioxidants such as oxidised vitamin Cand E, thereby protecting organelles, cells and tissue from the damaging effects of excessive ROS production (Taherian et al, 2019). Moringa oleifera leaves are also rich in vitamin C and E (Ajantha et al, 2018), which have been indicated as major chain-breaking antioxidants with strong reducing potential that attenuate free radical generating reactions (Brash & Havre, 2002).…”

“…Data were represented as mean ± SEMof 200 spermatozoa from 34 healthy donors their involvement in male fertility regulation are highly dependent on cofactors such as Zn, Se, Mg, Mn, Fe 3+ and Cu (Dobrakowski et al, 2018) whose presence in MO leaves has been validated (Dilawar et al, 2018). Centrifugation during the processing of semen increases the generation of ROS results in a loss in sperm motility and viability and induces DNA damage in spermatozoa (Henkel et al, 2012;Taherian et al, 2019). Semen samples obtained from normozoospermic men, washed and centrifuged with sperm washing media, in the presence of alpha-lipoic acid (0.02 mM) maintained sperm viability, and motility through the reduction of ROS production as well as prevention DNA damage (Taherian et al, 2019).…”

“…Centrifugation during the processing of semen increases the generation of ROS results in a loss in sperm motility and viability and induces DNA damage in spermatozoa (Henkel et al, 2012;Taherian et al, 2019). Semen samples obtained from normozoospermic men, washed and centrifuged with sperm washing media, in the presence of alpha-lipoic acid (0.02 mM) maintained sperm viability, and motility through the reduction of ROS production as well as prevention DNA damage (Taherian et al, 2019).…”