α-L-fucosidase from bull seminal plasma: Its purification and acrosome reaction promoting property

Srivastava, Pranay; Arbtan, Kaveh D.; Takei, Gerald H.; Huang, Tian-Sen; Yanagimachi, Ryuzo

doi:10.1016/0006-291x(86)90333-5

Cited by 29 publications

(13 citation statements)

References 14 publications

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“…The involvement of sperm associated a-fucosidase in the binding of spermatozoa to the egg coat has been demonstrated in mollusc 12 in ascidians 13 in mammals such as bull 14 and more recently the presence of the enzyme has been demonstrated in the plasma membrane of human spermatozoa. 26,27 Our data clearly revealed a time-associated loss in the activity of a-fucosidase post infection.…”

Section: Discussionmentioning

confidence: 97%

“…Absence of the enzyme or gross deficiency in some animals (dogs and humans) results in the genetic neurovisceral storage disease fucosidosis. 11 The involvement of sperm-associated 13 in mammals such as the bull 14 and has been proposed in amphibians. 15 Sialic acids occur as terminal components of cell surface glycoproteins and glycolipids and are found in some viruses, bacteria and protozoa and in large numbers of higher animals.…”

Section: Introductionmentioning

confidence: 99%

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Semen sialic acid surge and modulation of α‐L‐fucosidase activity: possible link to loss in reproductive capacity during trypanosomiasis

Oluyinka

Mairo

Ajanusi

et al. 2007

Cell Biochemistry & Function

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The profiles of semen sialic acid and the enzyme alpha-L-fucosidase were studied in rams undergoing chronic infection by Trypanosoma congolense. Our data showed a significant surge in the level of sialic acid with parasitaemia. The pattern followed a polynomial function we had reported for erythrocyte sialic acid in mice undergoing acute infection by T. congolense. The activity of the enzyme alpha-fucosidase decreased progressively with approximately 60% decrease at the end of the 14 weeks of infection. Representative semen samples from the control and infected rams were subjected to kinetic characterization. While the uninfected semen sample showed two active pH peaks at 4.5-5.5 and at 6.8-7.2, respectively, there was an apparent shift to only a single pH optimum at 4.5-5.5 for the pathological semen. The fucosidases from both sources were optimally active at 35 degrees C albeit with contrasting activation energies (E(a)) with values 20.58 and 35 kJ/mol for the control and infected semen, respectively. Kinetic studies using methylumbelliferyl-beta-fucoside (4MU-Fuc) as substrate gave K(M) and V(max) values of 3.25 microM and 14.6 micromol. min(-1) mg(-1), respectively for the control semen. The values for the infected semen were 18.25 microM and 10.5 micromol. min(-1) mg(-1), respectively. The significance of these results is discussed as they relate to loss in reproductive capacity in trypanosomoses.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Semen sialic acid surge and modulation of α‐L‐fucosidase activity: possible link to loss in reproductive capacity during trypanosomiasis

Oluyinka

Mairo

Ajanusi

et al. 2007

Cell Biochemistry & Function

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“…The cauda epididymidis is the main source of a-L-fucosidase in the bull [72]. Moura et al [48] also detected a-L-fucosidase in the cauda epididymal fluid of bulls having molecular weight 54.4 kd and pI 6.6 and was asscociated with high-fertility bulls.…”

Section: Some Others Seminal Plasma Proteinsmentioning

confidence: 89%

“…a-L-fucosidase is detected in seminal plasma, epididymal fluid and on spermatozoa of bull [35,72]. The cauda epididymidis is the main source of a-L-fucosidase in the bull [72].…”

Section: Some Others Seminal Plasma Proteinsmentioning

confidence: 97%

“…a-L-fucosidase is possibly involved in the modification of carbohydrate moieties of sperm membrane proteins during epididymal transit and was found in lower amounts in the seminal plasma of bulls with more percentage of abnormal sperm [35]. The pure enzyme promoted the acrosome reaction of guinea pig spermatozoa in vitro [72].…”

Section: Some Others Seminal Plasma Proteinsmentioning

confidence: 99%

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Seminal Plasma Proteome: Promising Biomarkers for Bull Fertility

Kumar

Singh

et al. 2012

Agric Res

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The seminal plasma is a mixture of secretions from the testis, epididymis and male accessory sex glands. A number of seminal plasma proteins are associated with male fertility but most of these have not been studied in detail till now. Recently, proteomics has been used to show the differences in proteins profile in seminal plasma from high and low fertility bulls. For example, osteopontin, phospholipase A2, P25b, acidic seminal fluid proteins, a-L-fucosidase and cathepsin D are positively correlated with fertility of bulls and may act as useful fertility marker (s), while lipocalin-type prostaglandin D synthase, spermadhesin Z13, clusterin and ubiquitin are negatively correlated with fertility in bulls. Bovine seminal plasma proteins in seminal plasma act like a double-edged sword and showed a quadratic association with bull fertility. The physiological roles of the metalloproteinase-2 (TIMP-2), ecto-ADP-ribosyltransferase 5, nuclobindin, Niemann-Pick C2 and epididymal sperm-binding protein 1 and their relationship to bull fertility need further studies. This review summarizes the physiological functions of proteins of seminal plasma and their relation to bull fertility.

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Antifertility factors of mammalian seminal fluid

Shivaji

Bhargava

1987

BioEssays

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The fertilizing ability of spermatozoa is inhibited by certain substances present in the seminal fluid. Most of these antifertility factors are proteinaceous in nature and differ in their physical characteristics. They inhibit fertilization by inhibiting either motility, capacitation, acrosome reaction or penetration of the ovum investments by the spermatozoa. This review describes and discusses the properties of these factors and their possible role, individually and collectively, in the regulation of fertility.

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α-L-fucosidase from bull seminal plasma: Its purification and acrosome reaction promoting property

Cited by 29 publications

References 14 publications

Semen sialic acid surge and modulation of α‐L‐fucosidase activity: possible link to loss in reproductive capacity during trypanosomiasis

Semen sialic acid surge and modulation of α‐L‐fucosidase activity: possible link to loss in reproductive capacity during trypanosomiasis

Seminal Plasma Proteome: Promising Biomarkers for Bull Fertility

Antifertility factors of mammalian seminal fluid

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