Antifertility factors of mammalian seminal fluid

Shivaji, Sisinthy; Bhargava, Poonam

doi:10.1002/bies.950070104

Cited by 32 publications

(15 citation statements)

References 26 publications

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“…Reports for several species suggest that seminal plasma contains factors that may influence male fertility [12]. These studies are generally based on comparisons of seminal plasma composition between males of differing fertility [37][38][39][40][41][42] or the isolation of factors from seminal plasma that facilitate or inhibit sperm capacitation, fertilization, or related events [43][44][45][46][47][48][49][50]. The existence of subfertile sires with apparent normal semen quality is an important observation and has stimulated the search for other markers of fertility [26].…”

Section: Discussionmentioning

confidence: 99%

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

2015

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Section: Discussionmentioning

confidence: 99%

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

2015

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“…Furthermore, neither the cooling procedure (second experiment) nor lipid efflux from sperm membrane affected sperm acrosomal integrity during incubation of semen. Seminal plasma contains factors that prevent premature capacitation, such as decapacitation factor and acrosome stabilizing factor [5,8]. Those factors may contribute to maintaining acrosomal integrity during incubation of undiluted or semen diluted with TG extender.…”

Section: Discussionmentioning

confidence: 99%

“…and that facilitate capacitation [4]. However, seminal plasma is also known to contain factors that are detrimental for sperm fertilizing ability [5], such as decapacitation factor [6][7][8] and motility inhibiting factor [9]. In addition, seminal plasma is detrimental to sperm storage [10] because it contains factors that negatively affect sperm viability [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Low-Density Lipoprotein Fraction from Hen’s Egg Yolk Decreases the Binding of the Major Proteins of Bovine Seminal Plasma to Sperm and Prevents Lipid Efflux from the Sperm Membrane1

Bergeron

Crête

Brindle

et al. 2004

Biology of Reproduction

167

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For sperm preservation, semen is generally diluted with extender containing egg yolk (EY), but the mechanisms of sperm protection by EY are unclear. The major proteins of bull seminal plasma (BSP proteins: BSP-A1/A2, BSP-A3, and BSP-30-kDa) bind to sperm surface at ejaculation and stimulate cholesterol and phospholipid efflux from the sperm membrane. Since EY low-density lipoprotein fraction (LDF) interacts specifically with BSP proteins, it is proposed that the sequestration of BSP proteins in seminal plasma by EY-LDF represents the major mechanism of sperm protection by EY. In order to gain further insight into this mechanism, we investigated the effect of seminal plasma, EY, and EY-LDF on the binding of BSP proteins to sperm and the lipid efflux from the sperm membrane. As shown by immunodetection, radioimmunoassays, and lipid analysis, when semen was incubated undiluted or diluted with control extender (without EY or EY-LDF), BSP proteins bound to sperm in a time-dependent manner, and there is a continuous cholesterol and phospholipid efflux from the sperm membrane. In contrast, when semen was diluted with extender containing EY or EY-LDF, there was 50%-80% fewer BSP proteins associated with sperm and a significant amount of lipid added to sperm membrane during incubation. In addition, sperm function analysis showed that the presence of EY or EY-LDF in the extender preserved sperm motility. These results show that LDF is the constituent of EY that prevents binding of the BSP proteins to sperm and lipid efflux from the sperm membrane and is beneficial to sperm functions during sperm preservation.

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“…These studies are. generally based on comparisons of seminal plasma composition between males of differing fertility [1][2][3][4][5][6] or the isolation of factors from seminal plasma that facilitate or inhibit sperm capacitation, fertilization, or related events [7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Fertility-Associated Proteins in Holstein Bull Seminal Plasma1

Killian

Chapman

Rogowski

1993

Biology of Reproduction

299

229

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This study was undertaken to determine whether bovine seminal plasma contained protein markers associated with bull fertility, and whether these markers were of value in predicting bull fertility. Seminal plasma was obtained from 35 Holstein bulls of known fertility. Two-dimensional PAGE of seminal plasma samples indicated that two proteins (26 kDa, pI 6.2; 55 kDa, pI 4.5) predominated in higher-fertility bulls, and two proteins (16 kDa, pI 4.1; 16 kDa, pI 6.7) predominated in lower-fertility bulls. Densitometry data for these proteins in individual samples were combined for bulls grouped by fertility level. Average density of the 26-kDa protein was significantly greater in seminal plasma of high-fertility bulls, and high-fertility seminal plasma also contained more of the 55-kDa protein than that of average- and below average-fertility bulls. Below average- and low-fertility bull seminal plasma had significantly more of both 16-kDa proteins than that of average- and high-fertility bulls. A regression model was developed to predict bull fertility using the four fertility-associated protein densities. A plot of actual bull fertility versus that calculated by this model was linear and positively correlated (r = 0.89). These findings indicate that bull seminal plasma contains fertility-associated proteins that are predictive of bull fertility.

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Antifertility factors of mammalian seminal fluid

Cited by 32 publications

References 26 publications

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

Low-Density Lipoprotein Fraction from Hen’s Egg Yolk Decreases the Binding of the Major Proteins of Bovine Seminal Plasma to Sperm and Prevents Lipid Efflux from the Sperm Membrane1

Fertility-Associated Proteins in Holstein Bull Seminal Plasma1

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