α-glucosidase Inhibition, DPPH Scavenging and Chemical Analysis of Polysaccharide Extracts of Aquilaria sp. Leaves

Abstract

Aquilaria sp. belongs to Thymelaeaceae family. This genus produces a unique fragrant wood locally known as ‘gaharu’. It is well known for usages in aromatherapy while very few scientific evidence on its usage as herbal medicine have been published. The current study was therefore conducted to determine the chemical characteristics as well as antioxidant and α-glucosidase inhibition potential of carbohydrate-rich extracts (aqueous and polysaccharide extracts) of three Aquilaria species leaves (A. malaccensis, A. sinensis and A. subintegra). The isolation of polysaccharide (PS) from the aqueous extract (AE) was conducted by ethanol precipitation prior to ultrasonic-assisted extraction. The total phenolic content (TPC), proximate and Fourier Transform Infrared Spectroscopy (FTIR) analysis were carried out to investigate the chemical characteristics, while the bioactivities determined were 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and α-glucosidase inhibition. As a result, AE of A. malaccensis leaves showed the strongest α-glucosidase (IC50: 0.094 mg/mL) and DPPH (IC50: 0.094 mg/mL) inhibition while contained more TPC (61.16 mg GAE/g) compared to other two species. Furthermore, PS of A. malaccensis leaves displayed even stronger α-glucosidase inhibition (IC50: 0.014 mg/mL) compared to its respective AE and other PS. In conclusion, this study primarily revealed the scientific evidence on the bioactive potential of PS of Aquilaria sp.

Effect of Karas (Aquilaria malaccensis) on Male Reproductive Organs and Sperm Quality in Adult Sprague Dawley Rats

“…After soaking, the mixture was placed in a sonicator (Wisd, Korea) for 20 min and filtered to remove the leaves powder. Filtrate was then centrifuged at 4000 rpm (25 ° C) for 20 min to collect the supernatant ( Rashid et al 2020 ). Extraction procedures were carried out three times and pooled filtrates were then concentrated at 40 ° C with a rotary vacuum evaporator (Heidolph, Germany) to separate the solvent from the crude extract ( Samsulrizal et al 2011 ).…”