ZZ/ZW sex chromosome system in an undescribed species of the genus &lt;i&gt;Apareiodon &lt;/i&gt;(Characiformes, Parodontidae)

Vicari, Marcelo Ricardo; Moreira‐Filho, Orlando; Artoni, Roberto Ferreira; Bertollo, Luiz Antônio Carlos

doi:10.1159/000093333

Cited by 38 publications

(56 citation statements)

References 43 publications

(31 reference statements)

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“…The family Parodontidae has been used in studies of sex chromosome differentiation, investigating the invasion of repetitive sequences and the increase in W chromosome size [Centofante et al, 2002;Rosa et al, 2006;Vicari et al, 2006;Bellafronte et al, 2009Bellafronte et al, , 2011Bellafronte et al, , 2012Schemberger et al, , 2014. In this study, in situ localization of the (GATA)n sequence revealed the accumulation of this microsatellite in proto-sex and sex chromosomes of 7 species: A. hasemani , A. ibitiensis , Apareiodon sp., P. hilarii , A. affinis , P. cf.…”

Section: Discussionmentioning

confidence: 58%

In situ Localization of (GATA)n and (TTAGGG)n Repeated DNAs and W Sex Chromosome Differentiation in Parodontidae (Actinopterygii: Characiformes)

Ziemniczak¹,

Traldi²,

Nogaroto³

et al. 2014

Cytogenet Genome Res

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The family Parodontidae presents a conserved diploid number of 54 chromosomes and different stages associated with ZW sex chromosome differentiation. For the great majority of species in this family it was proposed that the karyotypic diversification is mostly due to repetitive DNA mobility and accumulation. In this study, 2 repetitive probes, (GATA)n and (TTAGGG)n, were used to assess probable mechanisms of chromosome diversification, especially those related to molecular differentiation of the W chromosome. Results showed that the (GATA)n sequence is involved in the differentiation of the W chromosome female-specific region of Parodontidae and that it is accumulated in diverse autosomes. The (TTAGGG)n repeat is part of the vertebrate telomere, and the presence of interstitial telomeric sites may help to identify chromosome rearrangements. However, in Parodontidae, no interstitial telomeric sites were detected. This study shows plasticity in the amount of the (GATA)n repeat in Parodontidae that may be involved in chromatin modifications and transcriptional control of the W chromosome, and the role of repetitive DNAs in genomic diversification in this fish family is discussed.

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Section: Discussionmentioning

confidence: 58%

In situ Localization of (GATA)n and (TTAGGG)n Repeated DNAs and W Sex Chromosome Differentiation in Parodontidae (Actinopterygii: Characiformes)

Ziemniczak¹,

Traldi²,

Nogaroto³

et al. 2014

Cytogenet Genome Res

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“…Vicari et al (2006) chromosomically characterized Apareiodon sp. collected in the Verde River, a tributary of the Tibagi River (Paraná) belonging to the hydrographic system of the upper Paraná River.…”

Section: Discussionmentioning

confidence: 99%

“…Subtle differences are present in the chromosome morphology (A. ibitiensis has 50 meta-submetacentric and 6 subtelocentric chromosomes) and the NORs are single in Apareiodon sp. and multiple in A. ibitiensis (Table 4) (MoreiraFilho et al, 1984(MoreiraFilho et al, , 1985Jesus & Moreira-Filho, 2000a;Vicari et al, 2006;Bellafronte et al, 2009). Also, in studies with satellite DNAs, Schemberguer et al (2011) considered Apareiodon sp.…”

Section: Discussionmentioning

confidence: 99%

“…It is a relatively small family that includes three genera: Parodon, Apareiodon, and Saccodon (Pavanelli, 2003). Cytogenetic studies on Parodontidae reveal that although the species of this family show a predominant diploid number of 54 chromosomes, the karyotypic macrostructure and genomic structure are very diversified, with distinct sex chromosome systems (MoreiraFilho et al, 1980;Jesus & Moreira-Filho, 2000a;Vicari et al, 2006;Rosa et al, 2006;Bellafronte et al, 2009;, structural polymorphism (Jorge & Moreira-Filho, 2000, interspecific and intraspecific variation in the number and position of nucleolus organizer regions on chromosomes (Moreira-Filho et al, 1984;Vicente et al, 2001;Bellafronte et al, 2009; and interspecific differences in the distribution of distinct satellite DNA families (Vicente et al, 2003;Bellafronte et al, 2011;Schemberger et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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DNA barcode of Parodontidae species from the La Plata river basin - applying new data to clarify taxonomic problems

et al. 2013

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In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the same species by some authors, showed 4.2% genetic divergence, reinforcing their are different species. Samples of A. affinis from the Uruguay and Paraguay rivers presented 0.3% genetic divergence, indicating a close relationship between them. However, these samples diverged 6.1% from the samples of the upper Paraná River, indicating that the latter represents a potentially new species. The results showed the effectiveness of the DNA barcoding method in identifying the analyzed species, which, together with the morphological and cytogenetic available data, help species identification.Nos últimos anos o DNA barcoding surgiu como uma ferramenta rápida, precisa e eficiente para identificar espécies. Considerando a dificuldade na identificação de algumas espécies de Parodontidae da bacia do rio da Prata e da ausência de dados moleculares para o grupo, testamos a eficácia do código de barras de DNA e discutimos a importância do uso de diferentes abordagens para resolver problemas taxonômicos. Oito espécies foram analisadas com sequencias parciais do gene citocromo c oxidase I. A distância genética média K2P intraespecífica foi de 0,04% comparado com 4,2% para distância genética média K2P interespecífica. As análises de distância mostraram dois pares de espécies com divergência genética K2P inferior a 2%, mas o suficiente para separar estas espécies. Apareiodon sp. e A. ibitiensis, consideradas a mesma espécie por alguns autores, mostraram 4,2% de divergência genética, confirmando serem espécies diferentes. Amostras de A. affinis dos rios Uruguai e Paraguai apresentaram 0,3% de divergência genética, indicando um maior grau de relação entre elas, no entanto, esses exemplares divergiram em 6,1% em relação aos exemplares do alto rio Paraná, o que indica que estes últimos representam uma espécie potencialmente nova. Os resultados mostraram a eficácia do método de DNA barcoding na identificação das espécies analisadas, os quais, em conjunto com os dados morfológicos e citogenéticos disponíveis auxiliam na identificação inequívoca das espécies.

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“…While some species apparently lack differentiated sex chromosomes, others bear a clear heteromorphic ZZ/ZW sex system. All these species appear to share the same sex chromosome differentiation by increasing heterochromatic segments and accumulation of repetitive DNAs in the W chromosome from an ancestral homomorphic pair [Centofante et al, 2002;Vicente et al, 2003;Rosa et al, 2006;Vicari et al, 2006]. A satellite DNA family composed of a 200-bp unit (pPh2004) was isolated from the genome of Parodon hilarii using genomic DNA restriction [Vicente et al, 2003].…”

Section: Accumulation Of Repetitive Dna Sequences In Fish Sex Chromosmentioning

confidence: 99%

Repetitive DNAs and Differentiation of Sex Chromosomes in Neotropical Fishes

Cioffi

Camacho²,

Bertollo³

2010

Cytogenet Genome Res

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The processes working on sex chromosome differentiation are still not completely understood. However, the accumulation of repetitive DNA sequences has been shown to be one of the first steps in the early stages of such differentiation. In addition, regions with suppressed or no recombination have a potential to accumulate these DNA sequences and, for this reason, the absence of recombination between the sex chromosomes favors, by itself, the accumulation of repetitive sequences on these chromosomes during evolution. The diversity of sex-determining mechanisms in fish, alongside with the absence of heteromorphic sex chromosomes in many species, makes this group a useful model to better understand evolutionary processes of sex chromosomes in vertebrates, considering that fish occupy the basal position in the phylogeny of this group. In this review we draw attention to a preferential accumulation and enrichment in repetitive DNAs in sex chromosomes of many neotropical fish species in comparison with autosomes. This phenomenon has been observed between both morphologically differentiated and nascent sex chromosome systems, which highlight the potential role of these sequences in the differentiation of fish sex chromosomes generating differences in morphology and size between them.

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ZZ/ZW sex chromosome system in an undescribed species of the genus <i>Apareiodon </i>(Characiformes, Parodontidae)

Cited by 38 publications

References 43 publications

In situ Localization of (GATA)n and (TTAGGG)n Repeated DNAs and W Sex Chromosome Differentiation in Parodontidae (Actinopterygii: Characiformes)

In situ Localization of (GATA)n and (TTAGGG)n Repeated DNAs and W Sex Chromosome Differentiation in Parodontidae (Actinopterygii: Characiformes)

DNA barcode of Parodontidae species from the La Plata river basin - applying new data to clarify taxonomic problems

Repetitive DNAs and Differentiation of Sex Chromosomes in Neotropical Fishes

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