2013
DOI: 10.1590/s1679-62252013000300003
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DNA barcode of Parodontidae species from the La Plata river basin - applying new data to clarify taxonomic problems

Abstract: In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04… Show more

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Cited by 32 publications
(24 citation statements)
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“…This approach has also been used successfully for the identification of fish species in a number of other studies (Hubert et al, 2008;de Carvalho et al, 2011;Ribeiro et al, 2012;Bellafronte et al, 2013). Considering that the Itapecuru, like many other Brazilian river basins, has considerable historic and economic value for the local riverside populations, and still contains a diverse fish fauna, the findings of the present study represent an important advance in the scientific understanding of the fauna of Maranhão.…”
Section: Discussionmentioning
confidence: 77%
“…This approach has also been used successfully for the identification of fish species in a number of other studies (Hubert et al, 2008;de Carvalho et al, 2011;Ribeiro et al, 2012;Bellafronte et al, 2013). Considering that the Itapecuru, like many other Brazilian river basins, has considerable historic and economic value for the local riverside populations, and still contains a diverse fish fauna, the findings of the present study represent an important advance in the scientific understanding of the fauna of Maranhão.…”
Section: Discussionmentioning
confidence: 77%
“…Genetic distance results from the mtDNA COI sequences indicate very high pairwise values among the analysed species groups of Characidium , except with respect to comparisons among populations of C. zebra (below 0.02). Numerous studies of characiform fishes have demonstrated high interspecific genetic distances using mtDNA, as for example in curimatids (Melo, Ochoa, Vari, & Oliveira, ), lebiasinids (Benzaquem, Oliveira, Silva Batista, Zuanon, & Porto, ), parodontids (Bellafronte, Mariguela, Pereira, Oliveira, & Moreira‐Filho, ), bryconids (Machado, Ishizuka, Freitas, Valiati, & Galetti, ) and characids (Melo et al, ; Silva, Melo, Oliveira, & Benine, ). Because nucleotide substitutions accumulate through time (i.e., older clades tend to accumulate more substitutions), it is likely that high distance values result from the old splitting of the most recent common ancestor of Characidium from other crenuchids during the Paleogene (Poveda‐Martínez et al, ), a relatively old timing for genus‐level cladogenesis in Neotropical fishes (López‐Fernández & Albert, ).…”
Section: Discussionmentioning
confidence: 99%
“…Originally, DNA barcoding analysis applied the cut-off threshold of 1% for the analyzed species delimitation (Ratnasingham, Hebert, 2007). In specific groups, such as the Neotropical fish, studies have used the barcoding threshold (approximately 2%) proposed by Ward (2009) to delimit a wide-range of species (Rosso et al, 2012;Bellafronte et al,. 2013;Pereira et al, 2013;Gomes et al, 2015;Shimabukuro-Dias et al, 2016;Díaz et al, 2016).…”
Section: Discussionmentioning
confidence: 99%
“…Total genomic DNA was extracted from fin tissues preserved in ethanol using a method described by Aljanabi, Martinez (1997). Partial sequences of the mitochondrial gene COI were amplified using the Fish-F1 and Fish-R1 primers (Ward et al, 2005), following the PCR method proposed by Bellafronte et al (2013). PCR products were visualized on a 1% agarose gel and purified with 20% PEG (Lis, 1980).…”
Section: Methodsmentioning
confidence: 99%