Zytotoxizität und Genotoxizität von Fluoriden in humaner Mukosa und Lymphozyten

Kleinsasser, Norbert; Weissacher, Herbert; Wallner, Barbara C.; Kastenbauer, E.; Harréus, Ulrich

doi:10.1055/s-2001-13760

Cited by 28 publications

(18 citation statements)

References 3 publications

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“…We assumed that these negative results were obtained because fluoride is not capable of forming adducts on DNA bases or those that intercalate in DNA secondary structure. Our results are consistent with those of other studies 12,13,17,24 . An earlier study conducted by our group reported that NaF did not induce DNA damage in oral mucosa cells in vitro either 21 .…”

Section: Discussionsupporting

confidence: 83%

“…Studies focusing on possible genotoxic effect of excess fluoride are contradictory and inconclusive 16 . According to some authors, fluoride does not induce DNA damage 10,13,18,22 . However, some authors have observed the genotoxic potential of fluoride in Drosophila melanogaster 7 as well as synergistic and antagonist effect with known genotoxins 20 .…”

Section: Introductionmentioning

confidence: 99%

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Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

et al. 2004

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Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents. DESCRIPTORS: Sodium fluoride; Comet assay; Mutagenicity tests. RESUMO:O flúor tem sido amplamente usado na Odontologia, pois é um agente profilático efetivo e específico contra a cárie dentária. Entretanto, o flúor em excesso pode representar perigos à saúde humana, especialmente por causar agressão ao material genético. Testes de genotoxicidade representam uma importante parte da pesquisa do câncer para a avaliação de risco de possíveis carcinógenos. Neste presente estudo, danos ao DNA associados à exposição ao flúor foram avaliados pelo teste de células individualizadas em gel de agarose (teste do cometa) in vitro. Células de ovário de hamster chinês foram expostas ao fluoreto de sódio (NaF) nas concentrações finais de 7 a 100 µg/ml, durante 3 h, a 37°C. Os resultados mostraram que o NaF não contribuiu para os danos no DNA em todas as concentrações testadas, conforme demonstrado pelas médias do momento da cauda e da intensidade da cauda dos cometas. Esses achados são clinicamente importantes, uma vez que representam uma importante contribuição para a correta avaliação do potencial risco à saúde associada à exposição aos agentes odontológicos. DESCRITORES: Fluoreto de sódio; Ensaio em cometa; Testes de mutagenicidade.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

et al. 2004

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“…However, when cells were allowed to interact with the two xenobiotics at the indicated concentrations, cell viability was significantly reduced. Kleinsasser et al 9 have previously found that F causes mucosal cell damage …”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, F has been shown to be toxic, not only to the skeletal system, but also to the soft tissues by multiple mechanisms, such as enzyme activity inhibition, reactive oxygen and nitrogen species generation, impairment of the antioxidant defense system, induction of inflammation, and apoptosis. [6][7][8][9][10] As AgNPs and F may be used concomitantly in dental practice, we studied the effects of co-exposure to these xenobiotics on human gingival fibroblasts. It is known that xenobiotic interaction may increase or decrease the toxic effect of one or many substances.…”

Section: Introductionmentioning

confidence: 99%

Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride

Inkielewicz‐Stępniak¹,

Santos-Martínez²,

Medina³

et al. 2014

IJN

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Background Silver nanoparticles (AgNPs) and fluoride (F) are pharmacological agents widely used in oral medicine and dental practice due to their anti-microbial/anti-cavity properties. However, risks associated with the co-exposure of local cells and tissues to these xenobiotics are not clear. Therefore, we have evaluated the effects of AgNPs and F co-exposure on human gingival fibroblast cells. Methods Human gingival fibroblast cells (CRL-2014) were exposed to AgNPs and/or F at different concentrations for up to 24 hours. Cellular uptake of AgNPs was examined by transmission electron microscopy. Downstream inflammatory effects and oxidative stress were measured by real-time quantitative polymerase chain reaction (PCR) and reactive oxygen species (ROS) generation. Cytotoxicity and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and real-time quantitative PCR and flow cytometry, respectively. Finally, the involvement of mitogen-activated protein kinases (MAPK) was studied using Western blot. Results We found that AgNPs penetrated the cell membrane and localized inside the mitochondria. Co-incubation experiments resulted in increased oxidative stress, inflammation, and apoptosis. In addition, we found that co-exposure to both xenobiotics phosphorylated MAPK, particularly p42/44 MAPK. Conclusion A combined exposure of human fibroblasts to AgNPs and F results in increased cellular damage. Further studies are needed in order to evaluate pharmacological and potentially toxicological effects of AgNPs and F on oral health.

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“…In this context, published studies focusing on the possible genotoxic effect of excess fluoride are contradictory and inconclusive [Li et al, 1988]. According to some authors, fluoride does not induce DNA damage [Kihlman, 1957;Kram, 1978;Martin, 1979;IARC, 1982;Li et al, 1989;Slamenova, 1992;Kleinsasser, 2001]. However, some authors have observed the mutagenic potential of fluoride in Drosophila melanogaster [Mohamed, 1968;Mohamed and Chandler, 1977;Gerdes, 1971], synergistic and antagonist effects with known mutagens [Voegel, 1973;Monsour et al, 1985] and neurotoxic effects in humans [Mullenix, 1996] and rats [Chen et al, 2002].…”

mentioning

confidence: 99%

No Relationship between Subchronic Fluoride Intake and DNA Damage in Wistar Rats

Ribeiro

Marques

Assis³

et al. 2004

Caries Res

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Fluoride has been widely used in dentistry because it is an effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on the genetic apparatus. Genotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in peripheral blood, oral mucosa and brain cells in vivo. Male Wistar rats were exposed to sodium fluoride (NaF) at a 0, 7 and 100 ppm dose for drinking water during 6 weeks. The results pointed out that NaF did not contribute to the DNA damage in all cellular types evaluated as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to the correct evaluation of the potential health risk associated with dental agents exposure.

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Zytotoxizität und Genotoxizität von Fluoriden in humaner Mukosa und Lymphozyten

Cited by 28 publications

References 3 publications

Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride

No Relationship between Subchronic Fluoride Intake and DNA Damage in Wistar Rats

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