2006
DOI: 10.1099/vir.0.81477-0
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Zygocactus virus X-based expression vectors and formation of rod-shaped virus-like particles in plants by the expressed coat proteins of Beet necrotic yellow vein virus and Soil-borne cereal mosaic virus

Abstract: Expression vectors were constructed from 35S promoter-containing full-length cDNA clones of Zygocactus virus X (ZVX). The expression of foreign genes was driven by the ZVX coat protein (cp) subgenomic promoter. It was successful only when the variable region downstream of the conserved putative promoter region GSTTAAGTT(X 12-13 )GAA was retained. Most of the ZVX cp gene, except for a short 39 part, was replaced by the corresponding sequence of the related Schlumbergera virus X (SVX) and its cp subgenomic promo… Show more

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Cited by 7 publications
(3 citation statements)
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“…Stability has been improved in other vectors with duplicated SGPs by using heterologous promoters to reduce the likelihood of recombination [59,63,65,66]. Accordingly, we generated a hybrid vector containing the SGPs from two distantly related PepMV isolates, PepMV-Sp13 and PepMV-Ps5.…”
Section: Discussionmentioning
confidence: 99%
“…Stability has been improved in other vectors with duplicated SGPs by using heterologous promoters to reduce the likelihood of recombination [59,63,65,66]. Accordingly, we generated a hybrid vector containing the SGPs from two distantly related PepMV isolates, PepMV-Sp13 and PepMV-Ps5.…”
Section: Discussionmentioning
confidence: 99%
“…Subsequently, many potexviruses have been engineered as expression vectors, including bamboo mosaic virus (BaMV) (Chen et al, 2017 ; Lin et al, 1996 ; Liou et al, 2014 ), Cymbidium mosaic virus (CymMV) (Hsieh et al, 2013 ), cassava common mosaic virus (CsCMV) (Tuo et al, 2021 ), Plantago asiatica mosaic virus (PlAMV) (Minato et al, 2014 ), narcissus mosaic virus (NMV) (Zhang et al, 2013 ), Alternanthera mosaic virus (AltMV) (Lim et al, 2010 ), Zygocactus X virus (ZVX) (Koenig et al, 2006 ), pepino mosaic virus (PepMV) (Abrahamian et al, 2021 ; Sempere et al, 2011 ), and FoMV (Bouton et al, 2018 ; Liu et al, 2016 ; Mei et al, 2016 , 2019 ). Of these nine potexvirus species, only FoMV, BaMV, PlAMV, and CymMV can infect monocot plants.…”
Section: Engineering Fomv For Vigs ...mentioning
confidence: 99%
“…As noted above, our original infectious clones of AltMV differed in symptom severity and relative levels of replication, based on differences in the RdRp and TGB1 sequences, with a single residue difference in TGB1 having major effects on the efficiency of RNA silencing suppression activity. The CP subgenomic RNA (sgRNA) promoter was identified by comparison to those of other potexviruses (Skryabin et al, 1988;Lee et al, 2000;Koenig et al, 2006) and to the region upstream of the TGB1 start codon; the TGB1 and CP sgRNA promoters were subsequently also determined for portulaca strain AltMV-MU (Putlaev et al, 2015). We modified the infectious clones by duplication of the CP sgRNA promoter, and insertion of a multiple cloning site (MCS) between the Triple Gene Block and the CP gene, with initial insertion of either eGFP or DsRed as fluorescent marker proteins; these initial infectious clones required in vitro transcription of infectious transcripts using the T7 RNA polymerase (Lim et al, 2010a).…”
Section: Utility As a Viral Vectormentioning
confidence: 99%