2011
DOI: 10.1186/1746-4811-7-6
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Development of expression vectors based on pepino mosaic virus

Abstract: BackgroundPlant viruses are useful expression vectors because they can mount systemic infections allowing large amounts of recombinant protein to be produced rapidly in differentiated plant tissues. Pepino mosaic virus (PepMV) (genus Potexvirus, family Flexiviridae), a widespread plant virus, is a promising candidate expression vector for plants because of its high level of accumulation in its hosts and the absence of severe infection symptoms. We report here the construction of a stable and efficient expressi… Show more

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Cited by 62 publications
(66 citation statements)
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“…The capability to express GUS protein (68 KDa) in a NMV system suggests that large transcription factors, such as bHLHs that are involved in the regulation of flavonoid biosynthesis pathways, could also be expressed with this system. The pattern of GFP spread (locally and systemically) found in this study suggests that NMV shows behaviors similar to those characterized for other Potex viruses [16,54,55]. This long period of stable protein expression, in combination with the ease of systemic infection, suggests that the NMV system may provide an efficient platform for over-expression, purification and sub-cellular localization of proteins of interest.…”
Section: Discussionsupporting
confidence: 75%
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“…The capability to express GUS protein (68 KDa) in a NMV system suggests that large transcription factors, such as bHLHs that are involved in the regulation of flavonoid biosynthesis pathways, could also be expressed with this system. The pattern of GFP spread (locally and systemically) found in this study suggests that NMV shows behaviors similar to those characterized for other Potex viruses [16,54,55]. This long period of stable protein expression, in combination with the ease of systemic infection, suggests that the NMV system may provide an efficient platform for over-expression, purification and sub-cellular localization of proteins of interest.…”
Section: Discussionsupporting
confidence: 75%
“…A range of virus-based plant transient expression systems has been developed for protein expression [13,16,35-39]. Plant viral vector systems have several advantages over other systems, such as rapid and systemic infections, and also avoid the risks associated with the use of animal pathogens for production of recombinant proteins or vaccines [11,40-43].…”
Section: Discussionmentioning
confidence: 99%
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“…Èç ëèòåðàòóðíûõ äàííûõ èçâåñòíî, ÷òî ïîâòîðÿ-þùèåñÿ ïîñëåäîâàòåëüíîñòè ãåíîìîâ âèðóñíûõ âåê-òîðîâ ìîãóò âûùåïëÿòüñÿ âñëåäñòâèå ðåïëèêàòèâ-íîé ðåêîìáèíàöèè [10]. Äàííûé ôàêò ìîaeåò áûòü ïðè÷èíîé îòñóòñòâèÿ çíà÷èòåëüíîãî óâåëè÷åíèÿ ýô-ôåêòèâíîñòè ýêñïðåññèè ÁÎ AltMV-MU â òðàíñ-Ðèñ.…”
Section: ðåçóëüòàòû è îáñóAeäåíèåunclassified
“…The first types of genetically modified organisms created using 2A to co-express multiple proteins were plants, initially as a research tool, but also to improve drought-resistance (Kwon et al, 2004); disease-resistance (François et al, 2004;Geu-Flores et al, 2009) and nutritional qualities (Randall et al, 2004). Plant virus vectors based on potato virus X (PVX), cowpea mosaic virus (CPMV), pepino mosaic virus (PepMV), and bean pod mottle virus (BPMV) have been engineered with F2A and used to produce functional recombinant proteins including vaccines and antibodies (Smolenska et al, 1998;Gopinath et al, 2000;Marconi et al, 2006;Zhang et al, 2010;Sempere et al, 2011).…”
Section: Gene Expression In Plantamentioning
confidence: 99%