Zwitterion-functionalized dendrimer-entrapped gold nanoparticles for serum-enhanced gene delivery to inhibit cancer cell metastasis

Targeted Combination of Antioxidative and Anti‐Inflammatory Therapy of Rheumatoid Arthritis using Multifunctional Dendrimer‐Entrapped Gold Nanoparticles as a Platform

“…Meanwhile, the zwitterionic modification of the Au DENPs with 1,3‐PS renders the polyplexes with serum‐enhanced EGFP gene transfection of cells, which can be further quantitatively validated via flow cytometry assay (Figure S9, Supporting Information, p < 0.01 for N/P ratio of 20:1). These data are in accordance with our earlier work, [ 26 ] implying that the modification of PEGylated α‐TOS (for L1) or m PEG (for L2) does not seem to greatly impact their gene delivery efficiency.…”

“…Recently, our group has embarked on the development of multifunctional PAMAM dendrimer‐entrapped gold NPs (Au DENPs) [ 20 ] for effective gene delivery toward cancer gene therapy, [ 21 ] stem cell osteogenesis, [ 22 ] and immunotherapy. [ 23 ] PAMAM dendrimers have been considered as one of the most powerful nanosystems that can not only be functionalized with PEG, [ 21 ] acetyl groups, [ 24 ] glycosyl groups, [ 25 ] zwitterions, [ 26 ] or targeting ligands for improved delivery applications, [ 22,27 ] but also be incorporated with multiple organic or inorganic components to achieve combination therapy such as cancer gene therapy/photothermal therapy [ 17c ] and gene therapy/chemotherapy. [ 28 ] In particular, the entrapped Au NPs within the internal cavities of dendrimers are beneficial to reserve their 3D conformation for improved gene delivery.…”

Novel zwitterionic vectors: Multi-functional delivery systems for therapeutic genes and drugs

“… Category Polymers Loaded gene Ref. Phosphorylcholine PMPC-PDMAEMA pDNA [64] , [180] PMPC-PDPA pDNA, siRNA [65] , [66] , [67] , [181] PMPC-PAEMA pDNA [175] PMPC-PDMAPAA-PSA pDNA [176] PMDN-HBs pDNA [177] PMPC-PDEA pDNA [178] , [179] P((MPC- co -AEMAm)- b -MPC) pDNA [183] Sulfobetaine PMPDSAH-PDMAEMA pDNA [184] PEI- g- PHEAA- b -MEO 2 MA pDNA [185] , [186] PEI- g -PHEAA- b -PMPDSAH pDNA [185] , [186] PSBMA-PAA pDNA [187] ZALs Cas9 mRNA and sgRNA [190] MPDSAH-MEO 2 MA-MPDSAH pDNA [197] Amino acids OEAL pDNA [188] Carboxybetaine PCB 20 -DSPE siRNA [191] , [192] CBAA-PAMAMA pDNA [196] …”

“…And entrapping gold nanoparticles into PAMAM dendrimers has been proved to preserve the 3D spherical shape of dendrimers for better condensation of pDNA and compensate the positive charges of amines partly to alleviate the cytotoxicity [195] . Therefore, Shi et al first synthesized CBAA and covalently grafted CBAA to the surface of G5 PAMAM dendrimers to entrap gold NPs, and finally got CBAA-functionalized Au DENPs as a vector for the delivery of hypermethylated in cancer 1 (HIC1) pDNA [196] . Because of the abundant terminal amines, PAMAM could condense pDNA strongly and escape from lysosomes via proton sponge effect.…”

Multicomponent Polymerization toward Cationic Polymers for Efficient Gene Delivery

“…[ 50,51 ] However, PEGylation usually decreases the transfection efficiency and introduces undesired immune effect. [ 52 ] The introduction of more hydroxyl groups to the polycations is considered as an ideal alternative to PEGylation. [ 53,54 ] To further expand the polycation scope, di‐ sec ‐amine containing hydroxyl groups were also employed to prepare the polymers, which contains three important moieties: tertiary amine, thioether, and hydroxyl groups.…”