Zur Ultrastruktur der Schweineleber bei 24stündiger einfacher hypothermer Konservierung

Abstract

6 porcine livers were preserved by simple hypothermia for 24 hrs.Ultrastructural alterations were studied at various intervals with electron microscopic and enzyme cytochemical methods. A modified 1%inger's solution with increased concentrations of potassium and glucose was used for initial hypothermic shorttime perfusion. In 3 of 6 cases cell stabilizers (chlorpromazine and prednisolone) were added to the perfusate.Severe ultrastructura] changes such as disorganization, fragmentation, vesiculation, and degranulation of endoplasmie reticulum, swelling of the matrix in mitochondria, aggregation, and margination of chromatin as well as rupture of the plasma membrane followed by the release of cellular constituents into the Diss6 spaces and the sinusoids appeared in several liver cells after 6--9 hrs of preservation. In a significant number of cells, however, they were observed only after 12 hrs of preservation. In lysosomes there were no changes for more than 18 hrs of preservation.Especially interesting were early regressive alterations of Kupffer cells leading frequently to cytolysis already after 6 hrs of preservation.The addition of chlorpromazine and prednisolone to the perfusate had no effect on the preservation of ultrastructure.

Elektronenmikroskopische Befunde w�hrend hypothermer Lagerungskonservierung der Leber

“…Schwere Sch~idigungszeichen, wie Cytoplasmaeinschliisse oder Membran-und Kernfragmentation, die als irreversibel gelten [11], konnten wir in unseren Untersuchungen nicht feststellen. Schwere Sch~idigungszeichen, wie Cytoplasmaeinschliisse oder Membran-und Kernfragmentation, die als irreversibel gelten [11], konnten wir in unseren Untersuchungen nicht feststellen.…”

Elektronenmikroskopische Befunde w�hrend hypothermer Lagerungskonservierung der Leber

“…Eine Zunahme der Lysosomen nach Leberkonservierung wurde verschiedentlich beobachtet [5,8]. Nach C-Lagerung ist sie am geringsten, nach R-Konservierung am gr6Bten.…”