Makroskopische und mikromorphologische Befunde zur Beurteilung der Vitalität extrakorporal perfundierter Schweinelebern

Abstract

Several biochemical reactions are used in evaluating viability of extracorporeal perfused livers. Simple and quick tests of organ function, however, are needed in therapeutic application in man. In 33 homologous porcine liver perfusions we found that accurate observation of the macroscopic aspect gave a good criterion of organ function. The combination with micro-morphologic findings, especially after perfusion with Indian ink, proved to be a reliable base for evaluating the functional state of the livers used.

Extracorporeal isolated pig liver perfusion: Influence of various blood primes on liver function

“…Despite striking ameliorations in extracorporeal liver perfusion, including the use of silastic tubes, precision roller pumps, efficient blood filters, and the so-called pump circulation of the liver which allowed to simulate physiologic conditions, the function of the isolated liver is highly dependent on the blood used in the ECC. In the literature, this prime volume of 1,000-1,500ml (600ml in our study) has been obtained by various procedures [1,4,7]. Heterologous blood has shown to be inferior to homologous blood, especially in long-term perfusions [7].…”

“…Due to the easy access, heterologous human blood or homologous blood collected in the slaughterhouse were used by various groups [1,4,7]. Both of these perfusates are fraught with disadvantages.…”