Abstract:After filtration the urine was concentrated to about 1/10 of the original volume. The urine was centrifuged for 15 min at 5000 rev/min. The supernatant was chromatographed on Bio-Gel P-2-with a 10% aqueous solution of ethanol. Then the glycosaminoglycan fraction was separated into seven further fractions by chromatography on Dowex 1 X 2 and elution with increasing concentration of sodium chlorid. The desalted fractions were analysed. From the analytical data presented no definite correlation between excretion … Show more
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