Zur quantitativen Aminos�urebestimmung in K�rperfl�ssigkeiten und Organextrakten nach Elutionschromatographie an Ionenaustauschers�ulen mit automatischer Registrierung der Ergebnisse

Biochemical Analysis

“…This work was extended, making use of rigidly controlled particle sizes (155), and semiautomatic procedures (158) to demonstrate the potentialities of fast flow rates in the separation of amino acids (156). Further methodological advances included a continuous polarographic determination of amino acids (36) discussed more fully in the section on voltametric methods, a semi-micro method for the determination of amino acids on cationic exchange columns (121), and automatic analytical procedures for amino acids after separation of cationic exchange columns (204,286,355,389).…”

“…The first paper (204) demonstrated the feasibility of splitting the stream of the column effluent, part for analysis, part for collection for further analysis or isolation procedures. It was also demonstrated that tissue extracts could be placed directly on an ion exchange column without preliminary removal of the proteins (115), providing a nonionic detergent was included in the developing buffers.…”

“…It should be recorded here that the first and second Labline Awards in Chromatography and Electrophoresis have gone to Strain (204) and Zech-meister (24), respectively. Both men have made outstanding contributions to our knowledge of chloroplast pigments by the judicious application of Tswett's method.…”