Zur Kenntnis der natürlichen Harnporphyrine.

Grotepass, W.

doi:10.1515/bchm2.1938.253.5-6.276

Cited by 16 publications

(4 citation statements)

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“…The findings in this case were as follows: from a number of standpoints, one of which was referred to in paper I of this series (7), which dealt with the normal coproporphyrin of the urine. It is evident that if an individual such as one of the two present cases contributed to a pool being collected from presumably normal individuals, as in Grotepass' study (13), the result would be affected to a very marked degree and would be interpreted erroneously. The complete lack of symptoms in these two individuals is of much interest since it casts considerable doubt on the belief that the symptoms of lead poisoning and porphyria are "porphyrinopathic," as Carrie and others have believed.…”

mentioning

confidence: 71%

Studies of Coproporphyrin. Iii. Idiopathic Coproporphyrinuria; A Hitherto Unrecognized Form Characterized by Lack of Symptoms in Spite of the Excretion of Large Amounts of Coproporphyrin 1

Watson¹,

Schwartz²,

Schulze³

et al. 1949

J. Clin. Invest.

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mentioning

confidence: 71%

Studies of Coproporphyrin. Iii. Idiopathic Coproporphyrinuria; A Hitherto Unrecognized Form Characterized by Lack of Symptoms in Spite of the Excretion of Large Amounts of Coproporphyrin 1

Watson¹,

Schwartz²,

Schulze³

et al. 1949

J. Clin. Invest.

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“…angehören (3,8,9,17). Damit konnten ältere, gleichlautende Befunde von GROTEPASS (18), BRÜGSCH (4) und KEHL (5) bestätigt werden. Nachdem die Leber neben den erythropoetischen Elementen des Knochenmarks den überwiegenden Anteil der normalerweise vom Organismus benötigten Tetrapyrrolchromoproteide liefert und eine Reihe --vor allem chronischer -Lebererkrankungen mit einer mehr oder weniger vermehrten Ausscheidung von Koptoporphyrin einhergehen, war es naheliegend die qualita-tiven und quantitativen Verhältnisse solcher Porphyrinurien einer näheren Untersuchung zu unterziehen.…”

unclassified

“…Die von uns untersuchten pathologischen Fälle umfaßten 12 Virushepatitiden, l akute gelbe Leberatrophie,18 Lebercirrhosen verschiedenen Clotten u. Doyen: Ausscheidung der Koproporphyrin-Isomere I und Ausmaßes und unterschiedlicher Genese, 12 schwere Eisenmangelanämien, 11 erworbene sideroachrestische Anämien, 4 Panmyelopathien, 4 hämolytische Anämien verschiedener Genese, l Cooky Anämie, 2 Leukämien, l M. Hodgkin und l schwere Blutungsanämie. Sämtliche Urine wurden für 24 Stunden streng im Dunkeln und kühl über etwa 5 g Natriumbikarbonat gesammelt.…”

unclassified

Über die Ausscheidung der Koproporphyrin-Isomere I und III bei Erkrankungen der Leber und des Blutes

Clotten¹,

Doyen²

1967

Clinical Chemistry and Laboratory Medicine

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54Gotten u. Doyen: Ausscheidung der Koproporphyrin-Isomere I und HI buffer pH 4.2 add 4.5 mg Na-alginate and heat until the alginate is dissolved. After cooling to room temperature add 50 mg o-tolidine 2HC1. Standard curve for Hgb: lyze freshly obtained erythrocytes with destilled water, centrifuge and determine Hgb-concentration in the supernatant with the cyanide method (1). Prepare dilutions from 1-10 yWg Hgb/m/ with saline and measure the peroxidase activity with the standard method. A straight line should be obtained by plotting max. absorbance over the amount of Hgb (compare fig. 6a). Standard curve for Hgb-Hp in the presence of Na-alginate : Prepare Hpsolution with 8 /ig pure Hp/1 m/ dcst. water (the actual amount of the Hp-preparation depends on its purity). Mix equal volumes of the Hp-solution and a standardized Hgb-solution, containing 8 g Hgb/m/. 1 ml of the mixture contains 4 g Hgb as Hgb-Hp^com-plex. Prepare dilutions 0.5-4 & Hgb/m/ and measure the peroxidase activity as described, but substitute the plain o-tolidinetartrate-buffer with the alginate containing solution. In plotting max. absorbance over the amount of Hgb a curve as shown in figure 6b will be obtained. Unknown urine: Dilute urine 1:500 with saline and use 1 m/in the standard method. If the max. absorbance is at least .1, read Hgbconcentration from standard curve and multiply by 500. If the Hgb-concentration in the urine is too low to permit a 1:500 solution (no measurable reaction), the urine has to be treated with sephadex. Prepare a Sephadex-G-50 column (0.8 X 20 cm) with 1% saline. To 0.5 ra/ urine add 2.5 mg Hp (calculated as pure Hp) and mix well. Put the 5.0 m/ on the Sephadex column, elute with 1% saline and collect 2.0 m/ fractions (approx. 10). Take an appropriate aliquot (up to 1.0 m/) of each fraction and measure the peroxidase activity using the reagent-buffer with Na-alginate. With aliquots less than 1.0 m/ make up the volume difference with saline. For each fraction the amount of Hgb per 2.0m/ fraction is calculated (standard curve c). The sum is the amount of Hgb per 5.0 m/ of urine. For each Sephadex column the percentage of recovery of Hgb-peroxidase is determined. Prepare Hgb-solution with 4 //g Hgb/m/ saline and add an equal volume of a Hp-solution with 4 § pure Hp/m/. From this mixture put 5.0 m/ (= 10 g Hgb as Hp-complex) on column and procede as described for urine. The percent recovery obtained from this determination is used to correct the value obtained for urines with this particular column. The columns can be washed with saline and used repeatedly. The excretion of coproporphyrin in normal health, in liver ailments of various origin, and in a series of blood ailments was studied by extraction, high voltage electrophoresis and paper chromatography, and isomers I and III were determined. In normal persons the urinary level was 42-181 (88.6 ±30.2) ^g/24 hr. (males) and 43-135 (70.8 j-28.1) ^g/24hr. total coproporphyrin. Coproporphyrin III was predominant in healthy men and women (64.8 and 62.2% resp.). In ailm...

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“…The urinary coproporphyrin was isolated and esterified by the method described by Grotepass,5 and the melting point of the methyl ester was determined with the Johns micro melting point apparatus manufactured by the Fisher Scientific Company. All these patients were on a completely meat-free diet throughout the period of observa¬ tion.…”

mentioning

confidence: 99%

Excretion of Coproporphyrin in Hepatic Disease

Nesbitt¹

1943

Arch Intern Med (Chic)

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Zur Kenntnis der natürlichen Harnporphyrine.

Cited by 16 publications

References 0 publications

Studies of Coproporphyrin. Iii. Idiopathic Coproporphyrinuria; A Hitherto Unrecognized Form Characterized by Lack of Symptoms in Spite of the Excretion of Large Amounts of Coproporphyrin 1

Studies of Coproporphyrin. Iii. Idiopathic Coproporphyrinuria; A Hitherto Unrecognized Form Characterized by Lack of Symptoms in Spite of the Excretion of Large Amounts of Coproporphyrin 1

Über die Ausscheidung der Koproporphyrin-Isomere I und III bei Erkrankungen der Leber und des Blutes

Excretion of Coproporphyrin in Hepatic Disease

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