Zur Identifizierung flüchtiger Substanzen aus biologischem Material mit Hilfe der CLSA (Closed Loop Stripping Apparatus)

Naturally occurring monoterpenoids in needles of Picea abies (L.) Karst

“…Pickett (1975) pointed out that part of the complexity of the terpene pattern of higher plants may often be due to secondary chemical reactions during the isolation procedure. Steam distillation is suspected to generate t~-terpinene, •-terpinene and oxygenated terpenes like terpinene-4-ol, terpinolene, linalool and borneol Pauly et al 1973;Lorbeer et al 1984). The quantities of these terpenes did not markedly increase in this study, compared with solvent extraction, possibly due to the adjustment of the pH to neutrality.…”

“…Jiittner (1986) and Lorbeer et al (1984) used closed loop stripping to overcome analytical problems with components enclosed in the solvent extract that might disturb gas-chromatographical analysis. Compared with our results, Lorbeer et al (1984) found A-3-carene instead of sabinene, as well as enhanced proportions of [3-pinene and terpinene-4-ol and a reduced proportion of myrcene. The terpene pattern described by Jiittner (1986) resembles that of the pentane extract, but the absolute quantities of terpenes extracted seem to be lower.…”

“…Recently, several authors have reported on isomerization, saponification, polymerization and other rearrangements taking place under distillation conditions (Hiltunen et al 1983;Koedam and Looman 1980;Weston 1984;Stahl 1984;Lorbeer et al 1984). In addition, pretreatment and concentration procedures used during extraction of plant tissue may alter the quantity and composition of extractable terpenoids.…”

“…The essential oil of Picea abies needles was not extensively studied. Two authors reported on this subject recently (Lorbeer 1984;Jiittner 1986), but isolated the terpenes by closed loop stripping. This method is suspected to favour the more volatile compounds (Hanover 1972).…”

Analysis of Flower and Pollen Volatiles

“…1984;Lorbeer et al 1984). In this set-up the air flow system is closed such that the same air is passed repeatedly over the sample and through the adsorbent cartridge.…”