ZrO2/CeO2/polyacrylic acid nanocomposites with alkaline phosphatase-like activity for sensing

Wu, Xiangchuan; Wei, Jinhui; Wu, Chengyuan; Lv, Guangping; Wu, Liangpeng

doi:10.1016/j.saa.2021.120165

Cited by 19 publications

(11 citation statements)

References 53 publications

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“…[ 169 ] Recently, Wu et al. [ 170 ] synthesized ZrO 2 /CeO 2 /polyacrylic acid nanocomposites with improved phosphatase‐like activity by distributing ZrO 2 in CeO 2 . Based on these nanocomposites, a colorimetric detection method of methyl parathion was proposed with great potential for analytical applications.…”

Section: Nanozyme‐implemented Immunoassaysmentioning

confidence: 99%

“…[167] Based on the phosphatase-mimicking activities of nanoceria, a nanoceria-based method was proposed to detect organophosphorus pesticides by hydrolyzing the pesticide to para-nitrophenol with bright yellow color. [169] Recently, Wu et al [170] synthesized ZrO 2 /CeO 2 /polyacrylic acid nanocomposites with improved phosphatase-like activity by distributing ZrO 2 in CeO 2 . Based on these nanocomposites, a colorimetric detection method of methyl parathion was proposed with great potential for analytical applications.…”

Section: Phosphatase Mimicsmentioning

confidence: 99%

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Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Wang

Xianyu

2022

Small Methods

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treatment of cancers, the second leading cause of death in the world that are hard to be treated in the terminal stages. [2] For instance, liver cancer is one of the most lethal cancers and has a poor prognosis with a 5-year survival rate of less than 20%. [3] The early detection and timely treatment of cancers are very important for the patients to save their lives. Microorganism contamination in food is the main cause of foodborne diseases and 70% of patients with foodborne illnesses get sick after taking foods or water contaminated with pathogenic microorganisms. [4] Viruses are one of the major causes of infectious diseases and various public health events like the acquired immunodeficiency syndrome (AIDS), the severe acute respiratory syndrome (SARS), and the coronavirus disease 2019 (COVID-19). [5] Therefore, the timely and rapid detection of microorganisms is of great importance to ensure human health and public safety. [6] In addition, the detections of small molecules including mycotoxins and exogenous pollutions play an important role in food safety. Mycotoxins such as aflatoxin B1, ochratoxin A, and tenuazonic acid are classified as naturally occurring carcinogens by the World Health Organization that are extremely harmful to both humans and animals. [7] They not only directly threaten human life by contaminating food, but also indirectly affect human health through the food chain by contaminating feeds of animals. [8,9] Commonly used immunoassays include enzyme-linked immunosorbent assay (ELISA), quantum dot fluorescence immunoassay, fluorescence polarization immunoassay, colloidal gold immunoassay, chemiluminescent immunoassay (CLIA), electrochemical immunoassay, and microarray-based immunoassay. In spite of the different formats of immunoassays, they share the same principle that relies on the specific binding between antigenic determinants and antibody recognition sites. Therefore, it is of great significance to prepare specific antibodies with high affinity towards the target molecules to improve the specificity of immunoassays. Conventional immunoassays generally use polyclonal and monoclonal antibodies, but both types of antibodies have limitations such as poor specificity, low stability, and complicated preparation. To meet these challenges, a new type of antibody called "nanobody" has emerged. Nanobody is the heavy-chain-only antibody with the advantages of small size, high specificity, mass expression and high stability, which can act as a substitute for conventional antibodies in immunoassays and a promising low-cost immuno-affinity detection reagent.Immunoassay as a rapid and convenient method for detecting a variety of targets has attracted tremendous interest with its high specificity and sensitivity. Among the commonly used immunoassays, enzyme-linked immunosorbent assay has been widely used as a gold standard method in various fields that consists of two main components including a recognition element and an enzyme label. With the rapid advances in nanotechnology, nanobodies and nanozym...

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Section: Nanozyme‐implemented Immunoassaysmentioning

confidence: 99%

Section: Phosphatase Mimicsmentioning

confidence: 99%

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Wang

Xianyu

2022

Small Methods

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“…In these assays, OPs are not only the analyte, but also act as a reagent to generate signals. The representative phosphatase-like nanozymes include Ce-based [103][104][105] and Zr-based materials [106][107][108]. For example, to realize the determination of pesticides, Wu et al prepared a ZrO 2 /CeO 2 /PAA nanocomposite with remarkable phosphatase-mimicking activity, which could degrade methylparaoxon to p-nitrophenol with a yellow color for colorimetric detection [105].…”

Section: Assays Based On Phosphatase-like Nanozymesmentioning

confidence: 99%

“…The representative phosphatase-like nanozymes include Ce-based [103][104][105] and Zr-based materials [106][107][108]. For example, to realize the determination of pesticides, Wu et al prepared a ZrO 2 /CeO 2 /PAA nanocomposite with remarkable phosphatase-mimicking activity, which could degrade methylparaoxon to p-nitrophenol with a yellow color for colorimetric detection [105]. By using the inner filter effect between p-nitrophenol and carbon nanodots, the target methyl-paraoxon could also be detected by a fluorescence method [104].…”

Section: Assays Based On Phosphatase-like Nanozymesmentioning

confidence: 99%

Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

Zhu

Liu

et al. 2021

Biosensors

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To improve the output and quality of agricultural products, pesticides are globally utilized as an efficient tool to protect crops from insects. However, given that most pesticides used are difficult to decompose, they inevitably remain in agricultural products and are further enriched into food chains and ecosystems, posing great threats to human health and the environment. Thus, developing efficient methods and tools to monitor pesticide residues and related biomarkers (acetylcholinesterase and butylcholinesterase) became quite significant. With the advantages of excellent stability, tailorable catalytic performance, low cost, and easy mass production, nanomaterials with enzyme-like properties (nanozymes) are extensively utilized in fields ranging from biomedicine to environmental remediation. Especially, with the catalytic nature to offer amplified signals for highly sensitive detection, nanozymes were finding potential applications in the sensing of various analytes, including pesticides and their biomarkers. To highlight the progress in this field, here the sensing principles of pesticides and cholinesterases based on nanozyme catalysis are definitively summarized, and emerging detection methods and technologies with the participation of nanozymes are critically discussed. Importantly, typical examples are introduced to reveal the promising use of nanozymes. Also, some challenges in the field and future trends are proposed, with the hope of inspiring more efforts to advance nanozyme-involved sensors for pesticides and cholinesterases.

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“…Additionally, MP is usually used as a representative model of OPs for the construction of fast and efficient methods to detect ultra-low OPs in agricultural products, food, and environment such as soil and river water. 5 Conventionally, the MP content is detected using methods such as liquid chromatography-mass spectrometry (LC-MS), 6 gas chromatography-mass spectrometry (GC-MS), 7 surface-enhanced Raman scattering (SERS), 8 fluorescent method, 9 and electrochemical method 10 in various matrices. Among them, the electrochemical method is more convenient, easy to operate, reagent-saving, environment-friendly, efficiency-improving, cost-saving and equipment-portable.…”

Section: Introductionmentioning

confidence: 99%

Rational design of a novel MOF-based ternary nanocomposite for effectively monitoring harmful organophosphates in foods and the environment

Wang

Guo³

et al. 2023

Anal. Methods

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ZrO2/CeO2/polyacrylic acid nanocomposites with alkaline phosphatase-like activity for sensing

Cited by 19 publications

References 53 publications

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

Rational design of a novel MOF-based ternary nanocomposite for effectively monitoring harmful organophosphates in foods and the environment

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