Zone electrophoresis of enteroviruses

Abstract

Zone electrophoresis in a sugar concentration gradient is proposed as a complementary method for the biological classification of the enteroviruses. Under standardized experimental conditions it was found that the majority of the Coxsackie A viruses and the polioviruses have a common, low electrophoretic mobility. sackie A viruses and the polioviruses have a common, low electrophoretic mobility. The impression was gained that the ECHO viruses show the highest electrophoretic mobility, though in some cases there is overlapping with the Coxsackie Bs. The mobility of both of these groups is considerably higher than that of the other two.Zone electrophoresis appears to be an excellent method for the purification of the enteroviruses prior to the production of specific precipitating antibodies.

Immunoelectrophoresis of poliovirus antigens

“…The only similarity to the faster components described by POLSGN et al (2) was the observation of arcs of N character at different distances from the origin with the poliovirus type 2 MEF 1 pH 8.2. The same phenomenon was earlier observed with ECHO virus type 6 N antigens pH 7.0 (6).…”

Identification and Typing of Viruses and Detection of Antiviral Antibodies by Counterimmunoelectrophoresis

“…However, in the case of polio viruses the precipitation lines were often formed adjacent to the antigen wells, a finding that could be explained on the basis of (1) the relatively low concentration of poliovirus-precipitating antigens total mass com pared to that of antibodies and (2) the low electrophoretic mobility of polioviruses. In fact among enteroviruses, although they all have a similar sedimentation constant reflecting their similar particle size, polioviruses exhibit the lowest electrophoretic mobility [16], Again in certain experi ments with Sindbis virus, precipitation lines were formed not in the inter- well area but beyond the antigen wells. This could also be explained as a result of (1) unbalanced antigen-antibody concentration and/or (2) an overall slow rate of mobility of the antigen towards the cathode resulting in its being overtaken by the antibody.…”

Electrophoresis

“…Viruses (1, 661) and bacteria ( 406) including E. coli (419, 739, 904), Salmonella paratyphi (296), B. cereus (637), diphtheria bacilli (685), mycobacteria (132), Candida albicans (93), Pseudomonas (193), and staphylococci (452) have been investigated. There has been a separation, in acrylamide gel, of the proteins of a slime mold (941).…”