Zonal Density Gradient Electrophoresis of Serum Albumin and Bacteriophages M13, φ X174, and MS2. Electrophoretic Mobility Measurement

Abstract

A method based on zonal density gradient electrophoresis is presented which permits rapid separation of proteins and bacteriophages and the estimation of their electrophoretic mobility. The electrophoretic mobility of serum albumin for different buffer solutions and pH values has been measured and found to be in good agreement with previous values from the literature. The electrophoretic mobility of albumin has also been measured in the presence of two neutral watersoluble polymers (polyvinyl alcohol (PVA). polyethylene oxide (Polyox)), and a charged polymer (hydrolyzed ethylene maleic anhydride (EMA)). The electrophoretic mobility of three bacteriophages (M13. MS2. QX174) has been estimated in a phosphate/glycine buffer at pH = 7.4 as well as in the presence of bovine serum albumin and bovine y-globulin. The electrophoretic separation of MI3 and @XI74 bacteriophages was accomplished.