Electrophoretic mobilities of proteins and protein mixtures in porous membranes

O’Connor, Andrea J.; Pratt, H.R.C.; Stevens, Geoffrey W.

doi:10.1016/0009-2509(95)00407-6

Cited by 29 publications

(24 citation statements)

References 18 publications

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“…A simple example would be the electrophoretic motion of a particle through a pore in a membrane, such as arises when modeling electrophoretic separation of proteins (2,3).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrophoretic Motion of a Spherical Particle with a Thick Double Layer in Bounded Flows

Shugai

Carnie

1999

Journal of Colloid and Interface Science

100

148

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“…A simple example would be the electrophoretic motion of a particle through a pore in a membrane, such as arises when modeling electrophoretic separation of proteins (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…boundary, where ⑀ 0 is the permittivity of the vacuum, ⑀ r is the dielectric constant of the solvent, is the viscosity, and Henry's function f ( x) is given by f ͑ x͒ ϭ 2 3 ϩ 1 6 x 2 e x ͑E 3 ͑ x͒ Ϫ E 5 ͑ x͒͒ [2] and E n ( x) is the exponential integral…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic Motion of a Spherical Particle with a Thick Double Layer in Bounded Flows

Shugai

Carnie

1999

Journal of Colloid and Interface Science

100

148

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“…Assuming that the current is mainly carried by the electrolyte ions and that the electrical conductivity within the membrane pores is the same as that in the solution, the electric field in the membrane,Ē, is linked to that in the bulk solution, E, by the following expression [20]:…”

Section: Mathematical Modelmentioning

confidence: 99%

“…The average electric field strength, E in the separation chamber is calculated from the following equation [20]:…”

Section: Experimental Procedures and Operating Conditionsmentioning

confidence: 99%

Study of the mass transfer phenomena involved in an electrophoretic membrane contactor

Galier

Balmann

2001

Journal of Membrane Science

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Electrophoretic separators, in which a porous membrane is used as a contactor, offer the possibility to scale up electrophoresis as well as to extend the field of application of electrodialysis to fractionate polyamino acids, peptides or small proteins for instance. This paper deals with the study of the mass transfer mechanisms involved in such electroseparation processes. On one hand, a theoretical approach is carried out. The different contributions to the mass transfer are considered in order to establish a relationship providing the solute concentration as function of the main parameters of the system, i.e. the operating conditions and the membrane, buffer and solute characteristics. In this expression, a partition coefficient is used to represent the interactions taking place at the membrane-solution interface. Then, an experimental study is performed with different representative solutes using a prototype apparatus in order to determine the dependence of the solvent and solute transfer with respect to the operating and physicochemical parameters of the system. The experimental results show the existence of a limiting electro-osmotic flux, the origin of which is explained. Then the partition coefficient is determined for any set of conditions by fitting the variations of the solute concentration calculated by the model with experimental ones. The dependence of the partition coefficient with respect to the solute and buffer characteristics, together with that of the transmission coefficient obtained during filtration experiments, shows that the main limitation with respect to the mass transfer is due to electrostatic interactions taking place at the membrane-solution interface.

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“…However, in practical situation, the efficiency of electrophoretic separation in an electrolyte solution has been observed to strongly depend on the duration of sample injection, 11 which in turn is related to the unsteady electro-osmotic flow ͑EOF͒ field. [12][13][14] Further, the cylindrical capillary has been widely used in biological chips such as micropumps, 15 capillary electrophoretic devices for separation of proteins, 16 and coaxial jet mixing devices used in chemical analysis and biomedical diagnostics. 17,18 Therefore, it is necessary to study the transient response of EOF in a cylindrical microcapillary containing a salt-free medium.…”

Section: Introductionmentioning

confidence: 99%

Transient electro-osmotic flow in cylindrical microcapillaries containing salt-free medium

Chang

2009

Biomicrofluidics

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A theoretical study on the transient electro-osmotic flow through a cylindrical microcapillary containing a salt-free medium is presented for both constant surface charge density and constant surface potential. The exact analytical solutions for the electric potential distribution and the transient electro-osmotic flow velocity are derived by solving the nonlinear Poisson-Boltzmann equation and the NavierStokes equation. Based on these results, a systematic parametric study on the characteristics of the transient electro-osmotic flow is detailed. The general behavior of transient electro-osmotic flow in a cylindrical tube is similar to that observed in a microchannel containing an electrolyte solution. However, the steady-state electroosmotic flow significantly deviates from the typical plug flow at higher surface charge and the rate of increase in the electro-osmotic mobility is strongly suppressed due to the effect of counterion condensation. In addition, the applicability limit of these solutions is also discussed.

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Electrophoretic mobilities of proteins and protein mixtures in porous membranes

Cited by 29 publications

References 18 publications

Electrophoretic Motion of a Spherical Particle with a Thick Double Layer in Bounded Flows

Electrophoretic Motion of a Spherical Particle with a Thick Double Layer in Bounded Flows

Study of the mass transfer phenomena involved in an electrophoretic membrane contactor

Transient electro-osmotic flow in cylindrical microcapillaries containing salt-free medium

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