Zona pellucida removal and vitrified blastocyst transfer outcome: a preliminary study

Fuchiwaki, Megumi; Hiraoka, Kenichiro; Horiuchi, Toshitaka; Murakami, Tomoyo; Kinutani, Masayuki; Kinutani, Kazuo

doi:10.1016/s1472-6483(10)60694-3

Cited by 22 publications

(12 citation statements)

References 36 publications

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“…The embryos were then transferred into 1 ml of 37˚C 0.5 mol/l sucrose solution for 3 min and washed twice in the base medium for 5 min. AH was performed using a previously described method (23). The average thickness of the zona pellucida was calculated by the numerical values measured at the 12, 3, 6, and 8 o'clock positions from the inside to the outside.…”

Section: Methodsmentioning

confidence: 99%

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Wang¹,

Wang

et al. 2016

Biomedical Reports

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Abstract. The aim of the present study was to determine whether the size of the zona pellucida (ZP) thinning area by laser-assisted hatching affected the potential development of vitrified-warmed embryos. A total of 196 vitrified-warmed cleavage-stage embryos (from 49 patients, four sister embryos per patient) were used in the study, i.e., four sister embryos from each patient were randomly assigned to four groups: A control group of embryos that were not zona-manipulated (zona intact, group A); one experimental group of embryos in which a quarter of the zona pellucida was thinned using laser-assisted ZP thinning (group B); a second experimental group of embryos in which half of ZP was thinned (group C); and a third group in which two-thirds of the ZP was thinned (group D). Subsequent blastocyst development was assessed. Microscopy was performed to study the hatching process of the embryos after zona thinning. The blastocyst formation rates were 71.43% in group A, 67.35% in group B, 65.31% in group C, and 51.02% in group D (groups B-D vs. group A, P=0.661, P=0.515, P= 0.038, respectively). The rates of complete hatching were 30.61% in group A, 38.78% in group B, 61.22% in group C, and 48.98% in group D (groups B-D vs. group A, P= 0.396, P= 0.002, P= 0.063, respectively). For a subgroup of patients, there was a significant difference in the complete hatching in all the groups for women aged <35 years (P=0.011), and there was a significant difference in the complete hatching in all the groups for secondary infertility women (P=0.022). There was no significant difference in the blastocyst formation rates in the different groups of women aged ≥35 years (P= 0.340). In addition, there was no significant difference in the complete hatching in the different groups among women aged ≥35 years (P= 0.492). The results of the present study showed that in vitrified-warmed embryo transfers at the cleavage-stage, and the two-thirds zona pellucida thinning group demonstrated a significantly decreased blastocyst formation rate compared with the control group, while the half zona pellucida thinning group demonstrated a significantly increased complete hatching rate compared with the control group, which may have a high value in clinical application.

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Section: Methodsmentioning

confidence: 99%

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Wang¹,

Wang

et al. 2016

Biomedical Reports

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“…As soon as warming of embryos was completed, assisted hatching was performed by the method as described previously, albeit with slight modifications [14,22]. Warmed embryos were placed under mineral oil within a 50 μl microdroplet of 37°C Sperm Washing Medium (Irvine) in a Petri dish and positioned on the phasecontrast inverted microscope stage.…”

Section: Assisted Hatchingmentioning

confidence: 99%

Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study

Hiraoka

Horiuchi

Kusuda

et al. 2009

J Assist Reprod Genet

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“…(4) The opening of 50 μm probably is not large enough for the hatching process. Large ZP opening (1/2 or 2/3 of the zona diameter) [10,34] or total removal of the ZP [35] resulted in higher delivery rates than partial opening. Whether the possibility of MZT can be reduced by enlarging the opening size or total removal of the ZP needs more clinical data.…”

Section: Discussionmentioning

confidence: 98%

Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

Ren

Liu

Chen

et al. 2013

J Assist Reprod Genet

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Purpose To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles. Methods A total of 160 women who underwent vitrifiedwarmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), or the TE group (AH performing at the site opposite to the ICM). AH with laser zona drilling was performed 20 or 30 min after thawing once the ICM can be detected. Clinical pregnancy rate, implantation rate, live birth rate and the occurrence rate of monozygotic twins (MZT) pregnancy after transfer of these two groups were compared. Results No significant difference was found in the clinical pregnancy rate (63.8 % vs. 67.5 %), implantation rate (51.7 % vs. 53.6 %) and live birth rate (57.5 % vs. 62.5 %) between the ICM group and the TE group. The occurrence rate of MZT was comparable between the two groups (3.9 % vs. 5.6 %). Conclusions The site of assisted hatching has no influence on the implantation, pregnancy and live birth rate in human vitrified-warmed blastocyst transfer cycles.

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Zona pellucida removal and vitrified blastocyst transfer outcome: a preliminary study

Cited by 22 publications

References 36 publications

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study

Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

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