Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study

Hiraoka, Kenichiro; Horiuchi, Toshitaka; Kusuda, T.; Okano, Shinichiro; Kinutani, Masayuki; Kinutani, Kazuo

doi:10.1007/s10815-009-9350-6

Cited by 37 publications

(28 citation statements)

References 26 publications

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“…The most common method to achieve better results is to obtain and transfer multiple embryos. The surplus embryos produced using ARTs may be cryopreserved for subsequent use (1). The success of cryopreservation may undoubtedly increase the cumulative pregnancy rates of ARTs.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, artificial thinning of the zona or drilling of the zona may improve the embryo's potential and thus improve the clinical outcome of the thawing cycle. Since the 1980s, assisted hatching (AH) has been used to improve the chances of implantation during ARTs (1).…”

Section: Introductionmentioning

confidence: 99%

“…Since LAH can be divided into artificial thinning of the zona and drilling of the zona, previous findings have shown that the former is better than that the latter in vitro (15,16). Hiraoka et al have shown that vitrification can increase the hardness of zona, and the embryo implantation and clinical pregnancy rates in the half ZP thinning FET cycle was superior to that of a quarter ZP, although these studies lacked a control group (1). However, to the best of our knowledge, no study has included a sufficient sample to properly evaluate the effect of LAH on assisted reproduction outcomes (1,17).…”

Section: Introductionmentioning

confidence: 99%

“…Hiraoka et al have shown that vitrification can increase the hardness of zona, and the embryo implantation and clinical pregnancy rates in the half ZP thinning FET cycle was superior to that of a quarter ZP, although these studies lacked a control group (1). However, to the best of our knowledge, no study has included a sufficient sample to properly evaluate the effect of LAH on assisted reproduction outcomes (1,17). In the present study, we used vitrified frozen-thawed sister embryos and performed laser-assisted ZP thinning to reduce a quarter, a half and two-thirds area of the zona in the experimental groups.…”

Section: Introductionmentioning

confidence: 99%

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Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Wang¹,

Wang

et al. 2016

Biomedical Reports

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Abstract. The aim of the present study was to determine whether the size of the zona pellucida (ZP) thinning area by laser-assisted hatching affected the potential development of vitrified-warmed embryos. A total of 196 vitrified-warmed cleavage-stage embryos (from 49 patients, four sister embryos per patient) were used in the study, i.e., four sister embryos from each patient were randomly assigned to four groups: A control group of embryos that were not zona-manipulated (zona intact, group A); one experimental group of embryos in which a quarter of the zona pellucida was thinned using laser-assisted ZP thinning (group B); a second experimental group of embryos in which half of ZP was thinned (group C); and a third group in which two-thirds of the ZP was thinned (group D). Subsequent blastocyst development was assessed. Microscopy was performed to study the hatching process of the embryos after zona thinning. The blastocyst formation rates were 71.43% in group A, 67.35% in group B, 65.31% in group C, and 51.02% in group D (groups B-D vs. group A, P=0.661, P=0.515, P= 0.038, respectively). The rates of complete hatching were 30.61% in group A, 38.78% in group B, 61.22% in group C, and 48.98% in group D (groups B-D vs. group A, P= 0.396, P= 0.002, P= 0.063, respectively). For a subgroup of patients, there was a significant difference in the complete hatching in all the groups for women aged <35 years (P=0.011), and there was a significant difference in the complete hatching in all the groups for secondary infertility women (P=0.022). There was no significant difference in the blastocyst formation rates in the different groups of women aged ≥35 years (P= 0.340). In addition, there was no significant difference in the complete hatching in the different groups among women aged ≥35 years (P= 0.492). The results of the present study showed that in vitrified-warmed embryo transfers at the cleavage-stage, and the two-thirds zona pellucida thinning group demonstrated a significantly decreased blastocyst formation rate compared with the control group, while the half zona pellucida thinning group demonstrated a significantly increased complete hatching rate compared with the control group, which may have a high value in clinical application.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Wang¹,

Wang

et al. 2016

Biomedical Reports

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“…However, there was no international standard protocol for AH technique. The size of the opening [9][10][11], the depth of the micro dissection in ZP [12,13] and the site of AH [14] may all influence the effectiveness of AH. A recent in-vitro observational study of vitrified blastocysts suggested the existence of polarity in the hatching process of human embryos [14].…”

Section: Introductionmentioning

confidence: 99%

Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

Ren

Liu

Chen

et al. 2013

J Assist Reprod Genet

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Purpose To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles. Methods A total of 160 women who underwent vitrifiedwarmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), or the TE group (AH performing at the site opposite to the ICM). AH with laser zona drilling was performed 20 or 30 min after thawing once the ICM can be detected. Clinical pregnancy rate, implantation rate, live birth rate and the occurrence rate of monozygotic twins (MZT) pregnancy after transfer of these two groups were compared. Results No significant difference was found in the clinical pregnancy rate (63.8 % vs. 67.5 %), implantation rate (51.7 % vs. 53.6 %) and live birth rate (57.5 % vs. 62.5 %) between the ICM group and the TE group. The occurrence rate of MZT was comparable between the two groups (3.9 % vs. 5.6 %). Conclusions The site of assisted hatching has no influence on the implantation, pregnancy and live birth rate in human vitrified-warmed blastocyst transfer cycles.

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Assisted hatching on assisted conception (in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI))

Carney

Sangeeta

Blake

et al. 2012

Cochrane Database of Systematic Reviews

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Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study

Cited by 37 publications

References 26 publications

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

Assisted hatching on assisted conception (in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI))

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