Zingiber mioga reduces weight gain, insulin resistance and hepatic gluconeogenesis in diet-induced obese mice

Abstract

Abstract. Zingiber mioga is a perennial herb belonging to the ginger family (Zingiberaceae) that is used medicinally to treat cough and rheumatism in China and consumed throughout Japan. The aim of the present study was to investigate the anti-obesity effects of Z. mioga following extraction with distilled water or 70% ethanol. In 3T3-L1 preadipocyte cells, Z. mioga water extract (ZMW) markedly inhibited adipogenesis, whereas the ethanol extract had no effect. In addition, we conducted ZMW feeding experiments (0.25 or 0.5% ZMW) in high-fat diet (HFD)-fed mice to examine the anti-obesity effects of Z. mioga in vivo. Body weight and serum triglyceride and cholesterol levels significantly decreased in the HFD + ZMW 0.5% group. Notably, ZMW decreased liver weight but not adipose tissue weight. Furthermore, insulin resistance and hepatic mRNA expression of gluconeogenic genes, such as phosphoenolpyruvate carboxykinase and G6Pase, were improved in the HFD + ZMW 0.5% group. Furthermore, ZMW treatment decreased hepatic lipogenic gene expression; however, it did not alter adipogenesis in fat tissue, suggesting that ZMW inhibits hepatosteatosis through the suppression of lipogenesis. ZMW improved HFD-induced hepatic inflammation. Collectively, the present findings suggest that ZMW may serve as a new and promising strategy for the treatment of hepatosteatosis.

Synergistic lipid‑lowering effects of Zingiber mioga and Hippophae rhamnoides extracts

“…In the present study, the inhibitory effects of the ZH mixture on lipid accumulation in vitro were examined and its anti-obesity effects in HFD-induced obese mice evaluated. The results indicated that treatment with individual extracts inhibited lipid accumulation in 3T3-L1 adipocytes, as shown in previous studies (19,25). This effect was found to be higher than predicted (using the equation proposed by Colby (29) following treatment with a mixture containing both materials.…”

“…Specifically, PPAR and C/EBPα are transcription factors that induce the expression of several genes involved in adipogenic differentiation (40). Previous studies have shown that ZM and HR inhibit fat accumulation by suppressing the expression of these genes (19,26). The results of the present study indicated that ZH treatment inhibited the expression of genes related to adipocyte differentiation in 3T3-L1 cells to a degree greater than that observed following treatment with either single extract.…”

“…It has been suggested to exhibit diverse biological functions, including antihyperglycemic and antioxidant activity, and to improve allergic asthma and memory (16)(17)(18). A previous study by our research group demonstrated that Zingiber mioga extract (ZM) exerted an anti-obesity effect in HFD-induced obese mice, and revealed the effects of ZM on insulin resistance and liver gluconeogenesis (19); however, only a high concentration of ZM showed a significant effect in these previous experiments. In order to reach an effective dose, an excessive intake of the extract was required; therefore, combining multiple nutritional supplements could be a useful method for achieving effects at lower concentrations.…”

Complete chloroplast genome of Zingiber mioga by de novo sequencing

“…(Zingiber mioga) is a Zingiberaceae family from China, Japan, and Korea. Its young flower buds and fruit has been used as a traditional food (Jo et al 2016), and it is used medicinally to treat the irregular menstruation, dysmenorrhea, cough, and asthma in China and consumed throughout Japan (Huang et al 2016;Lee et al 2016;. Moreover, Zingiber mioga also has important medical and natural pigment value (Huang and Li 2013;Huang et al 2016).…”

Protective Effects of 2-Dodecyl-6-Methoxycyclohexa-2,5 -Diene-1,4-Dione Isolated from Averrhoa Carambola L. (Oxalidaceae) Roots on High-Fat Diet-Induced Obesity and Insulin Resistance in Mice

“…After a 15-hour fast (5:00 pm-8:00 am), the mice were injected intraperitoneally with glucose (2 g/kg of body weight). The blood glucose levels at 0, 15, 30, 60, and 120 min were measured using an accurate blood glucose meter (Accu-check Performa, Roche, Germany) [15][16][17][18]. The area under the curve (AUC) was calculated using the trapezoid rule.…”