Synergistic lipid‑lowering effects of Zingiber mioga and Hippophae rhamnoides extracts

Abstract

The effects of a mixture of Hippophae rhamnoides (HR) and Zingiber mioga (ZM) extract (ZH) on intracellular lipid accumulation were investigated in vitro and the anti-obesity effects of ZH evaluated in mice with high-fat diet-induced obesity. The results revealed that ZH inhibited lipid accumulation in 3T3-L1 adipocytes and Huh-7 cells by suppressing adipogenic and lipogenic gene and protein expression. To evaluate the anti-obesity effects of ZH, mice fed a high-fat diet were orally administered low and high doses of ZH (low, ZM 400 mg/kg + HR 100 mg/kg; high, ZM 800 mg/kg + HR 200 mg/kg) for 9 weeks. ZH significantly reduced body weight gain and adipose tissue accumulation with no reduction in food intake when compared to control treatment. Furthermore, ZH reduced hepatic triglyceride and total cholesterol levels, as well as adipose cell size, in the liver and epididymal fat pads, respectively, through inhibition of adipogenesis and lipogenesis-related gene expression. These results suggested that ZH inhibits lipid accumulation, thereby indicating its potential for use as a new therapeutic strategy for obesity.

Synergistic Hypolipidemic Effects and Mechanisms of Phytochemicals: A Review

“…For example, Park et al showed that treatment with the combination of Hippophae rhamnoides and Zingiber mioga extracts inhibited the expression of genes associated with adipocyte differentiation in 3T3-L1 cells more than any single extract treatment, and the combination inhibited lipid accumulation in hepatocytes more effectively than the single extract. However, which phytochemicals of Hippophae rhamnoides and Zingiber mioga have synergistic effects needs to be further investigated [ 27 ]. Similarly, Yari et al investigated the clinical efficacy of flaxseed and hesperidin in non-alcoholic fatty liver disease and observed significant synergistic effects of the combination on the regulation of lipid metabolism [ 28 ].…”