Zinc sulfide-chitosan hybrid nanoparticles as a robust surface for immobilization of Sillago sihama α-amylase

Bahri, Sarah; Homaei, Ahmad; Mosaddegh, Elaheh

doi:10.1016/j.colsurfb.2022.112754

Cited by 35 publications

(13 citation statements)

References 46 publications

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“…Obviously, when the temperature was set at 80 °C, the relative activity of immobilized enzymes was almost twice that of free enzymes. Thus, compared to free α-Amy, FHA showed higher catalytic activity in basic solution or at higher temperature that was basically the same as previous studies. , …”

Section: Resultssupporting

confidence: 89%

“…Thus, compared to free α-Amy, FHA showed higher catalytic activity in basic solution or at higher temperature that was basically the same as previous studies. 36,37 Altered catalytic performance of FHA was somewhat ascribed to the change of α-Amy structure after immobilization. There might be three reasons: (1) the cross-linking reaction between α-Amy and amino group-modified Fe 3 O 4 @ HZIF-8 nanoparticle could enhance the rigidity of enzyme structure and limit the free motion of enzyme; (2) the spatial structure of Fe 3 O 4 @HZIF-8 carrier, especially the presence of mesopores with a size of about 50.0 nm, could provide a certain microenvironment for the enzyme reaction; (3) hierarchical micro-and mesopores on the HZIF-8 shell could offer rich surfaces and channels for fast mass transfer, facilitate affinity of enzymes and substrates.…”

Section: Effect Of Ph Andmentioning

confidence: 99%

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Magnetic Amine-Functionalized ZIF-8 with Hierarchical Pores for Efficient Covalent Immobilization of α-Amylase

Zhang

Sun

et al. 2023

ACS Appl. Nano Mater.

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The immobilization strategy between ZIF-8 and different enzymes has focused on in situ encapsulation and pore infiltration, while the surface attachment has been seldom reported because of less functional groups on the ZIF-8 surface. Here, a magnetic amine-functionalized ZIF-8 with hierarchical pores was designed for efficient covalent immobilization of α-amylase (α-Amy). By using Fe3O4 nanoparticles as the core and ZIF-8 as the shell, followed by Au3+–Zn2+ exchange reaction and β-mercaptoethylamine modification, the magnetic hollow ZIF-8 nanoparticle with amine groups on the surface was synthesized. With the assistance of glutaraldehyde, an α-Amy-magnetic hollow ZIF-8 composite was prepared and fully characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, energy-dispersive X-ray spectroscopy element mapping analysis, and vibration sample magnetometry. Then, the catalytic activity of immobilized α-Amy was explored with a K m of 2.307 mg/mL and V max of 0.506 mg/mL·min, indicating excellent catalytic activity. The study about stability and reusability demonstrated that the catalytic activity of immobilized α-Amy remained above 50% after 10 times of reuse. Thus, the designed magnetic amine-functionalized ZIF-8 was verified to be a nice carrier for covalent enzyme immobilization.

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Section: Resultssupporting

confidence: 89%

Section: Effect Of Ph Andmentioning

confidence: 99%

Magnetic Amine-Functionalized ZIF-8 with Hierarchical Pores for Efficient Covalent Immobilization of α-Amylase

Zhang

Sun

et al. 2023

ACS Appl. Nano Mater.

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“…By carefully combining copper ions and cysteine (Cys), Tang et al [ 134 ] synthesized biomimetic copper–cysteine nanoparticles (Cu/CysNPs) with a stronger laccase-like activity than natural laccase ( Figure 6 D,E). The approach of immobilizing an enzyme on zinc sulfide–chitosan hybrid nanoparticles was discovered by Bahri et al [ 135 ] and it considerably improved the materials’ thermal and storage stabilities, and resistance to pH extremes.…”

Section: One-step Synthesis For Enzyme Assembliesmentioning

confidence: 99%

Advances in the One-Step Approach of Polymeric Materials Using Enzymatic Techniques

et al. 2023

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The formulation in which biochemical enzymes are administered in polymer science plays a key role in retaining their catalytic activity. The one-step synthesis of polymers with highly sequence-controlled enzymes is a strategy employed to provide enzymes with higher catalytic activity and thermostability in material sustainability. Enzyme-catalyzed chain growth polymerization reactions using activated monomers, protein–polymer complexation techniques, covalent and non-covalent interaction, and electrostatic interactions can provide means to develop formulations that maintain the stability of the enzyme during complex material processes. Multifarious applications of catalytic enzymes are usually attributed to their efficiency, pH, and temperature, thus, progressing with a critical structure-controlled synthesis of polymer materials. Due to the obvious economics of manufacturing and environmental sustainability, the green synthesis of enzyme-catalyzed materials has attracted significant interest. Several enzymes from microorganisms and plants via enzyme-mediated material synthesis have provided a viable alternative for the appropriate synthesis of polymers, effectively utilizing the one-step approach. This review analyzes more and deeper strategies and material technologies widely used in multi-enzyme cascade platforms for engineering polymer materials, as well as their potential industrial applications, to provide an update on current trends and gaps in the one-step synthesis of materials using catalytic enzymes.

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“…The idea of enzymes’ immobilization was introduced by Nelson and Griffin in 1916 after they noticed that invertase can hydrolyze sucrose after being absorbed onto charcoal . Since then, several reversible/irreversible methods of enzyme immobilization were introduced that can enhance the physicochemical properties of enzymes, enabling them for applied usages . The reversible immobilization of enzymes entails adsorption, metal binding, and ionic binding.…”

Section: Introductionmentioning

confidence: 99%

“… 15 Since then, several reversible/irreversible methods of enzyme immobilization were introduced that can enhance the physicochemical properties of enzymes, enabling them for applied usages. 16 The reversible immobilization of enzymes entails adsorption, metal binding, and ionic binding. Yet, the irreversible immobilization of enzymes comprises entrapment and covalent binding.…”

Section: Introductionmentioning

confidence: 99%

Enzyme Immobilization Technologies and Industrial Applications

et al. 2023

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Enzymes play vital roles in diverse industrial sectors and are essential components of many industrial products. Immobilized enzymes possess higher resistance to environmental changes and can be recovered/recycled easily when compared to the free forms. The primary benefit of immobilization is protecting the enzymes from the harsh environmental conditions (e.g., elevated temperatures, extreme pH values, etc.). The immobilized enzymes can be utilized in various large-scale industries, e.g., medical, food, detergent, textile, and pharmaceutical industries, besides being used in water treatment plants. According to the required application, a suitable enzyme immobilization technique and suitable carrier materials are chosen. Enzyme immobilization techniques involve covalent binding, encapsulation, entrapment, adsorption, etc. This review mainly covers enzyme immobilization by various techniques and their usage in different industrial applications starting from 1992 until 2022. It also focuses on the multiscale operation of immobilized enzymes to maximize yields of certain products. Lastly, the severe consequence of the COVID-19 pandemic on global enzyme production is briefly discussed.

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Zinc sulfide-chitosan hybrid nanoparticles as a robust surface for immobilization of Sillago sihama α-amylase

Cited by 35 publications

References 46 publications

Magnetic Amine-Functionalized ZIF-8 with Hierarchical Pores for Efficient Covalent Immobilization of α-Amylase

Magnetic Amine-Functionalized ZIF-8 with Hierarchical Pores for Efficient Covalent Immobilization of α-Amylase

Advances in the One-Step Approach of Polymeric Materials Using Enzymatic Techniques

Enzyme Immobilization Technologies and Industrial Applications

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