Zinc Properties in Weakening the Pathogenic Effects of Excess Cobalt Intake

Abstract

The high level of anthropogenic environmental pollution in urban areas leads to the accumulation of the body and the development of diseases. In connection with the foregoing, the study focuses on the features of changing the parameters of systemic hemodynamics in conditions of combined and isolated administration of cobalt and zinc salts. Cobalt chloride (4 mg/kg) and zinc (20 mg/kg) were administered intragastrically to Wistar rats via an atraumatic probe daily for one month. Blood pressure was determined by catheterization of the femoral artery. The catheter was filled with a 10 % heparin solution and connected to the DDA electrometer of the monitor MX-04 (Russia). Minute blood volume was measured by thermodilution, for which an MT-54M thermistor was inserted through the left common carotid artery into the aortic arch. A saline solution of fixed room temperature with a volume of 0.2 ml was injected into the right atrium through a catheterized right yarmine vein. The mean arterial pressure (MAP), cardiac index (CI), stroke index (SI) and specific peripheral vascular resistance (SVR) were calculated. The concentration of ionized calcium was determined using an AEK-01 electrolyte analyzer (Russia). The concentration of total calcium was determined spectrophotometrically using the sets "Calcium Arsenazo", "LLC" "Agat-Med" (Moscow, Russia). The cobalt content was determined after preliminary sample preparation on a Kvant-AFA atomic absorption spectrophotometer (Russia). Studies have shown monthly intragastric administration of cobalt or zinc chloride can observe hypokinetic arterial hypertension. According to the analyses zinc weakens the pathogenic effect of cobalt on the parameters of systemic hemodynamics within their combined introduction. The study also revealed that cobalt with increased intake accumulates in bone tissue, leading to its decalcification, while the content of ionized calcium increases in the blood.