Zinc intestinal absorption in newborn rats at 21 day postpartum: Effects of maternal ethanol consumption

Abstract

This study was designed to examine the effects of prenatal and postnatal exposure of ethanol in the in vivo absorption of zinc in the small intestine in newborn rats at the 21st day after birth. The rats were accustomed to increasing amounts of ethanol (5 to 20%, vol/vol) in tap water for 1 month. During pregnancy and suckling period, ethanol-fed dams were assigned again to ethanol 20% in drinking water. Two sets of experiments were performed. In the first set, jejunal zinc absorption in control group and litters nursed by dams receiving ethanol showed a gradual increase along with the increase of perfusion time at all the assayed concentrations. In general, in litters of ethanol-fed dams, jejunal zinc absorption expressed as nmol/intestinal surface was higher than in control animals. In the second set of experiments, distal ileum zinc absorption in offspring of ethanol-fed dams showed a significantly decrease at all concentrations tested. The results showed that intestinal parameters measured in jejunum and distal ileum of litters exposed to ethanol were always significantly less than in control newborn. These results indicate that exposure of rats to ethanol during the pregnancy and suckling period, may affect postnatal development of intestinal functions and decrease the distal ileum zinc absorption in pups at the end of the lactation period.

Effects of folic acid and amino acids supplementation on zinc intestinal absorption in the progeny of ethanol-treated rats

“…In previous work (24) we showed that the values for zinc jejunal absorption were only significantly different at 25 and 500 uM concentrations in pups of dams exposed to ethanol. Ileal zinc absorption showed a significant decrease in pups of ethanol-fed dams as compared to control animals at all assayed concentrations.…”

“…Zinc absorption.-The intestinal zinc absorption was assayed using the method previously described (24). Briefly, Zn as ZnCl was dissolved in a Tyrode medium whose composition (gil) was: NaCI This solution was pre-warmed (37 DC) before perfusion.…”

Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

“…Inflow and outflow cannulas were tied into the intestine. After cannulation, the loop was rinsed with 0.9% NaCl solution, replaced inside the body wall and perfused as previously described by Tavares et al (1998) [22]. A flow rate of 3.0 ml/min during a period of 5 min was used for each concentration in all animals.…”

Efeitos de bebidas alcóolicas em mães lactantes e suas repercussões na prole

“…39 O efeito da ingestão de álcool por quatro dias no período inicial (quinto ao oitavo dia) e médio (nono ao décimo segundo dia) de amamentação também provocou redução da lactação e baixo consumo de leite pelas crias. 63 Alguns autores sugerem que esta resposta se deve à modificação imediata do aroma do leite. 32 Em animais cujas mães consumiram continuamente etanol na gestação e lactação, alterações na absorção intestinal de zinco no íleo distal são observadas no fim do período de aleitamento.…”

“…32 Em animais cujas mães consumiram continuamente etanol na gestação e lactação, alterações na absorção intestinal de zinco no íleo distal são observadas no fim do período de aleitamento. 63 Nestas condições de alcoolismo, as mães são afetadas duplamente, apresentando redução ponderal e alteração na composição nutritiva do leite (elevação de fosfatidilserina); por outro lado não foram observadas mudanças no crescimento linear da ninhada. 53 Resultados diferentes foram encontrados por Detering et al, 22 estudando o efeito do etanol a 35% administrado em uma dieta líquida consumida na gestação e lactação; ratos recém-nascidos mostraram retardo do crescimento físico (incluindo retardo do sistema nervoso central) em maior proporção do que as alterações causadas por privação nutricional.…”