Zinc Finger Protein CG9890 - New Component of ENY2-Containing Complexes of Drosophila

Abstract

In previous studies, we showed that the insulator protein Su(Hw) containing zinc finger domains interacts with the ENY2 protein and recruits the ENY2-containing complexes on Su(Hw)-dependent insulators, participating in the regulation of transcription and in the positioning of replication origins. Here, we found interaction between ENY2 and CG9890 protein, which also contains zinc finger domains. The interaction between ENY2 and CG9890 was confirmed. It was established that CG9890 protein is localized in the nucleus and interacts with the SAGA, ORC, dSWI/SNF, TFIID, and THO protein complexes. KEYWORDS ENY2, CG9890, drosophila, immunoprecipitation, zinc fingers. ABBREVIATIONS ENY2 -enhancer of yellow 2; C2H2 -zinc fingers of C2H2 type; SAGA -histone acetyltransferase complex; SWI/SNF -chromatin remodeler; AMEX -mRNA export complex; ORC -origin recognition complex.