Zika virus infection induces MiR34c expression in glioblastoma stem cells: new perspectives for brain tumor treatments

Iannolo, Gioacchin; Sciuto, Maria Rita; Cuscino, Nicola; Pallini, Roberto; Douradinha, Bruno; Vitiani, Lucia Ricci; Maria, Ruggero De; Conaldi, Pier Giulio

doi:10.1038/s41419-019-1499-z

Cited by 25 publications

(38 citation statements)

References 32 publications

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“…The previous study reported GSCs to respond differently to targeted therapy. 183 Since various strategies such as nanoparticles 184,185 and Zika virus [186][187][188] can be applied to target GSCs, it is critical to treat GSCs with combined therapeutic options to improve treatment efficacy. A recent study summarized the efficacy of strategy that by targeting SOX2 on stem-like cells can inhibit tumor progression.…”

Section: Immunotherapymentioning

confidence: 99%

The adaptive transition of glioblastoma stem cells and its implications on treatments

Wang

Zhang

et al. 2021

Sig Transduct Target Ther

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Glioblastoma is the most malignant tumor occurring in the human central nervous system with overall median survival time <14.6 months. Current treatments such as chemotherapy and radiotherapy cannot reach an optimal remission since tumor resistance to therapy remains a challenge. Glioblastoma stem cells are considered to be responsible for tumor resistance in treating glioblastoma. Previous studies reported two subtypes, proneural and mesenchymal, of glioblastoma stem cells manifesting different sensitivity to radiotherapy or chemotherapy. Mesenchymal glioblastoma stem cells, as well as tumor cells generate from which, showed resistance to radiochemotherapies. Besides, two metabolic patterns, glutamine or glucose dependent, of mesenchymal glioblastoma stem cells also manifested different sensitivity to radiochemotherapies. Glutamine dependent mesenchymal glioblastoma stem cells are more sensitive to radiotherapy than glucose-dependent ones. Therefore, the transition between proneural and mesenchymal subtypes, or between glutamine-dependent and glucose-dependent, might lead to tumor resistance to radiochemotherapies. Moreover, neural stem cells were also hypothesized to participate in glioblastoma stem cells mediated tumor resistance to radiochemotherapies. In this review, we summarized the basic characteristics, adaptive transition and implications of glioblastoma stem cells in glioblastoma therapy.

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Section: Immunotherapymentioning

confidence: 99%

The adaptive transition of glioblastoma stem cells and its implications on treatments

Wang

Zhang

et al. 2021

Sig Transduct Target Ther

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“…Cellular viability was assessed by plating the cells in 96 well non-treated plates (Corning, New York, NY, USA) and, after the indicated time, the cells were collected and lysed with Cell Titer Glo Luminescent Cell Viability Assay reagent (Promega, Mannheim, Germany), in accordance with the provider’s indications. Luminescence was analyzed using the Spark Microplate Reader (Tecan, Männedorf, Switzerland) as described elsewhere [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Klebsiella pneumoniae Lipopolysaccharides Serotype O2afg Induce Poor Inflammatory Immune Responses Ex Vivo

et al. 2021

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Currently, Klebsiella pneumoniae is a pathogen of clinical relevance due to its plastic ability of acquiring resistance genes to multiple antibiotics. During K. pneumoniae infections, lipopolysaccharides (LPS) play an ambiguous role as they both activate immune responses but can also play a role in immune evasion. The LPS O2a and LPS O2afg serotypes are prevalent in most multidrug resistant K. pneumoniae strains. Thus, we sought to understand if those two particular LPS serotypes were involved in a mechanism of immune evasion. We have extracted LPS (serotypes O1, O2a and O2afg) from K. pneumoniae strains and, using human monocytes ex vivo, we assessed the ability of those LPS antigens to induce the production of pro-inflammatory cytokines and chemokines. We observed that, when human monocytes are incubated with LPS serotypes O1, O2a or O2afg strains, O2afg and, to a lesser extent, O2a but not O1 failed to elicit the production of pro-inflammatory cytokines and chemokines, which suggests a role in immune evasion. Our preliminary data also shows that nuclear translocation of NF-κB, a process which regulates an immune response against infections, occurs in monocytes incubated with LPS O1 and, to a smaller extent, with LPS O2a, but not with the LPS serotype O2afg. Our results indicate that multidrug resistant K. pneumoniae expressing LPS O2afg serotypes avoid an initial inflammatory immune response and, consequently, are able to systematically spread inside the host unharmed, which results in the several pathologies associated with this bacterium.

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“…Total RNA was extracted using the miRNeasy Isolation Kit (Qiagen, Hilden, Germany). Sequencing libraries were prepared according to the Illumina Protocol for small RNA (Illumina, San Diego, CA, USA release Feb. 2014), as previously described 18 . One microgram of total RNA was processed using the small RNA library kit, as described by the manufacturer (Illumina).…”

Section: Methodsmentioning

confidence: 99%

miRNA expression analysis in the human heart: Undifferentiated progenitors vs. bioptic tissues—Implications for proliferation and ageing

Iannolo

Sciuto

Cuscino

et al. 2021

J Cellular Molecular Medi

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In developed countries, cardiovascular diseases are currently the first cause of death. Cardiospheres (CSs) and cardiosphere‐derived cells (CDCs) have been found to have the ability to regenerate the myocardium after myocardial infarction (MI). In recent years, much effort has been made to gain insight into the human heart repair mechanisms, in which miRNAs have been shown to play an important role. In this regard, to elucidate the involvement of miRNAs, we evaluated the miRNA expression profile across human heart biopsy, CSs and CDCs using microarray and next‐generation sequencing (NGS) technologies. We identified several miRNAs more represented in the progenitors, where some of them can be responsible for the proliferation or the maintenance of an undifferentiated state, while others have been found to be downregulated in the undifferentiated progenitors compared with the biopsies. Moreover, we also found a correlation between downregulated miRNAs in CSs/CDCs and patient age (eg miR‐490) and an inverse correlation among miRNAs upregulated in CSs/CDCs (eg miR‐31).

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Zika virus infection induces MiR34c expression in glioblastoma stem cells: new perspectives for brain tumor treatments

Cited by 25 publications

References 32 publications

The adaptive transition of glioblastoma stem cells and its implications on treatments

The adaptive transition of glioblastoma stem cells and its implications on treatments

Klebsiella pneumoniae Lipopolysaccharides Serotype O2afg Induce Poor Inflammatory Immune Responses Ex Vivo

miRNA expression analysis in the human heart: Undifferentiated progenitors vs. bioptic tissues—Implications for proliferation and ageing

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