Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis

Liu, Weiping; Chen, Qi; Liu, Zhidan; Weng, Zhiwei; Nguyen, Truong Nam; Feng, Jiaming; Zhou, Shaohu

doi:10.1016/j.jep.2021.113789

Cited by 14 publications

(6 citation statements)

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“…FOXOs are deemed as downstream targets of PI3K/AKT, and FOXO phosphorylation is induced by phosphorylated AKT, which leads to suppression of FOXO transcription [ 40 ]. There has been convincing evidence manifesting that Zihuai recipe alleviated CTX-DOR by suppressing PI3K/AKT pathway-mediated ovarian cell apoptosis [ 7 ]. Likewise, Dingkun Pill enhanced ovarian reserve by suppressing the PI3K/AKT/mTOR signaling pathway in DOR rats [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…Granulosa cell apoptosis has been shown to be disordered in DOR, which has significant consequences on the generation of empty follicles, oocyte retrieval, and the quality of oocytes and embryos [ 6 ]. Currently, hormone replacement therapy (HRT) is a commonly used treatment for pathologic DOR, but the long-term outcome of HRT is poor [ 7 ]. Although there have been a number of studies on DOR, the optimal treatment for pathologic DOR is still undetermined.…”

Section: Introductionmentioning

confidence: 99%

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Rehmannioside D mitigates disease progression in rats with experimental-induced diminished ovarian reserve via Forkhead Box O1/KLOTHO axis

Yan

Wang

Chen

et al. 2023

Korean J Physiol Pharmacol

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This study aims to explore the impact of Rehmannioside D (RD) on ovarian functions of rats with diminished ovarian reserve (DOR) and its underlying mechanisms of action. A single injection of cyclophosphamide was performed to establish a DOR rat model, and fourteen days after the injection, the rats were intragastrically administrated with RD for two weeks. Rat estrus cycles were tested using vaginal smears. Ovarian tissues were histologically evaluated, the number of primordial, mature, and atretic follicles was calculated, and the apoptotic rate of granulosa cells. Follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E 2 ) levels were determined by ELISA assays. Protein levels of Forkhead Box O1 (FOXO1), KLOTHO, Bcl-2, and Bax were investigated in ovarian tissues of DOR rats. The binding between FOXO1 and KLOTHO was verified by ChIP assay. High-dose administration of RD into DOR rats improved their estrus cycles, increased ovarian index, enhanced the number of primordial and mature follicles, reduced the number of atretic follicle number, and ovarian granulosa cell apoptosis in addition to inhibiting FSH and LH levels and upregulating E 2 expression. FOXO1 and KLOTHO were significantly suppressed in DOR rats. FOXO1 knockdown partially suppressed the protective effects of RD on DOR rats, and KLOTHO overexpression could restore RD-induced blockade of DOR development despite knocking down FOXO1. FOXO1 antibody enriched KLOTHO promoter, and the binding between them was reduced in DOR group compared to that in sham group. RD improved ovarian functions in DOR rats and diminished granulosa cell apoptosis via the FOXO1/KLOTHO axis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rehmannioside D mitigates disease progression in rats with experimental-induced diminished ovarian reserve via Forkhead Box O1/KLOTHO axis

Yan

Wang

Chen

et al. 2023

Korean J Physiol Pharmacol

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“…After oral administration, it is absorbed the gastrointestinal tract and degraded in the liver. [13] The Foley balloon has the function of filling the patient's uterine cavity, covering the wound and forming a biological barrier, avoiding uterine adhesions during the patient's postoperative recovery process, and is conducive to the repair of endometrial hyperplasia. [14] According to the results of this study, after receiving different treatment regimens for 2 months, compared with the control group, the observation group achieved higher curative effect, shorter abdominal pain duration, vaginal bleeding duration and refluid time, and lower vaginal bleeding.…”

Section: Discussionmentioning

confidence: 99%

“…After oral administration, it is absorbed through the gastrointestinal tract and degraded in the liver. [13] The Foley balloon has the function of filling the patient’s uterine cavity, covering the wound and forming a biological barrier, avoiding uterine adhesions during the patient’s postoperative recovery process, and is conducive to the repair of endometrial hyperplasia. [14]…”

Section: Discussionmentioning

confidence: 99%

To study the current status of uterine adhesions after fetal residue and the preventive effect of using estrogen and progesterone sequential therapy combined with Foley balloon

Liu¹,

Wang²,

Li³

et al. 2022

Medicine

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To investigate the current status of uterine adhesions in patients with residual fetus and analyze the preventive effect of estrogen and progesterone sequential therapy combined with Foley balloon. Eighty-six patients with residual fetus were divided into control group and observation group. On the basis of the treatment plan of the control group, the observation group received estrogen and progesterone sequential therapy combined with Floey balloon treatment. Clinical efficacy, postoperative recovery status (abdominal pain duration, vaginal bleeding duration, vaginal bleeding volume, refluid time), the incidence of intrauterine adhesions, uterine hemodynamics (uterine artery systolic maximum blood flow rate [Vmax], end diastolic blood flow rate [Vmin], resistance index [RI]), and the probability of complications were evaluated. Compared with the control group, the observation group achieved higher curative effect (P < .05); the observation group had shorter duration of abdominal pain, vaginal bleeding and refluid time, and lower vaginal bleeding (P < .05); the probability of intrauterine adhesions is lower (P < .05); before treatment, there is little difference in the levels of Vmax, Vmin, RI, and other indicators between the 2 groups (P > .05). After treatment, compared with the control group, the observation group Vmax, Vmin, and other indicators, the level was significantly lower, and the RI level was significantly higher (P < .05); the observation group had a lower probability of complications (P < .05). The likelihood of uterine adhesions after fetal remains is increased. The use of estrogen and progesterone sequential therapy in conjunction with Foley balloon therapy can improve treatment efficacy, improve uterine adhesion prevention, and promote patient recovery after surgery.

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“…Statistical comparisons were analyzed by one-way analysis of variance (ANOVA). A value of p < 0.05 suggests a difference, and p < 0.01 represents a significant difference ( Chen et al, 2019 ; Liu et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Pharmacological effects of Bufei Jianpi granule on chronic obstructive pulmonary disease and its metabolism in rats

et al. 2022

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This work was performed to determine the pharmacological effects of Bufei Jianpi granules on chronic obstructive pulmonary disease and its metabolism in rats.Chronic obstructive pulmonary disease (COPD), ranked as the third leading cause of death worldwide, is seriously endangering human health. At present, the pathogenesis of COPD is complex and unclear, and the drug treatment mainly aims to alleviate and improve symptoms; however, they cannot achieve the purpose of eradicating the disease. Bufei Jianpi granule (BJG) is a Chinese medicine developed by the First Affiliated Hospital of Henan University of Traditional Chinese Medicine for treating COPD. This study focuses on the pharmacological effects of BJG on COPD and its metabolism in rats, aiming to provide a scientific basis for developing BJG against COPD. A total of 72 Sprague–Dawley (SD) rats were divided into the blank group, model group, positive control group, and BJG groups (2.36, 1.18, and 0.59 g/kg). Except for the blank group, rats in other groups were administered lipopolysaccharide (LPS) combined with smoking for 6 weeks to establish the COPD model. After another 6 weeks of treatment, the therapeutic effect of BJG on COPD rats was evaluated. In the BJG (2.36 g/kg) group, the cough condition of rats was significantly relieved and the body weight was close to that of the blank group. Compared with the mortality of 16.7% in the model group, no deaths occurred in the BJG (2.36 g/kg) and (1.18 g/kg) groups. The lung tissue damage in the BJG groups was less than that in the COPD group. Compared with the model group, MV, PIF, PEF, and EF50 in the BJG groups were observably increased in a dose-dependent manner, while sRaw, Raw, and FRC were obviously decreased. Also, the contents of IL-6, IL-8, TNF-α, PGE2, MMP-9, and NO in the serum and BALF were lowered dramatically in all BJG groups. All indicators present an obvious dose–effect relationship. On this basis, the UPLC-QTOF-MS/MS technology was used to analyze characteristic metabolites in rats under physiological and pathological conditions. A total of 17 prototype and 7 metabolite components were detected, and the concentration of most components was increased in the COPD pathologic state. It is suggested that BJG has a pharmacological effect in the treatment of COPD and the absorption and metabolism of chemical components of BJG in rats exhibited significant differences under physiological and pathological conditions.

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Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis

Cited by 14 publications

References 50 publications

Rehmannioside D mitigates disease progression in rats with experimental-induced diminished ovarian reserve via Forkhead Box O1/KLOTHO axis

Rehmannioside D mitigates disease progression in rats with experimental-induced diminished ovarian reserve via Forkhead Box O1/KLOTHO axis

To study the current status of uterine adhesions after fetal residue and the preventive effect of using estrogen and progesterone sequential therapy combined with Foley balloon

Pharmacological effects of Bufei Jianpi granule on chronic obstructive pulmonary disease and its metabolism in rats

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