Needle penetration resistance (NPR), screw withdrawal resistance (SWR), core drilling (CD) and drilling chips extraction (DCE) are nondestructive and semi-destructive techniques used to estimate density in timber structures. In most of the previous studies, these techniques were tested in clear sawn timber and clear specimens. The goal of the present paper is to study the relationship between density and these techniques by means of five different devices in whole pieces of timber from built-in engineering structures, which are from 12 4.5-m long structural timber joists of Norway spruce from a 19thcentury building in Barcelona (Catalonia, Spain). Although determination coefficients (R2) for density estimation models were lower than those from clear timber, the results obtained confirmed that these four techniques are suitable forin-situdensity estimation of woods in buildings. The best results were obtained by CD (the bigger the bit, the higher the correlation), followed by DCE, and SWR. The worst correlation was found for NPR devices, but the results could be probably improved with more measurements.