Zellstoffwechsel und Zellteilung

Lettré, Hans

doi:10.1007/bf00524727

Cited by 24 publications

(3 citation statements)

References 7 publications

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“…Since divalent cations are indispensable as activators of various metabolic processes involving ATP, including oxidative phosphorylation (14), their removal might be expected to decrease the availability of energy derived from breakdown of ATP. In the view of Lettr6 (15,18) the drop in available energy would account for loss of membrane rigidity and produce rounding of the cell. If the limiting conditions for energy production are conceived as localized to or near the cell surface, the redistribution of the activating ions from the cell interior or from a proper exterior medium would reverse the effects.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years Lettr6 and coworkers have reported a series of experiments which relate these phenomena to changes in localized concentrations of adenosine triphosphate (ATP). Specifically, the peripheral ATP level, assumed to provide the energy necessary to maintain cell form during interphase, becomes reduced at mitosis, resulting in a relaxation of the cell surface that leads to cell rounding and intermittent bubbling (15,18). The principal evidence is derived from the experimental suppression of cell rounding and anaphasic surface motility by addition of ATP to the culture medium, and by the induction of such surface or cortical activity in interphase cells when grown in an anaerobic environment, or when exposed to mitochondrlal poisons or agents that inhibit respiration or phosphorylation (16,17).…”

Section: Introductionmentioning

confidence: 99%

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Surface Responses in Cultured Fibroblasts Elicited by Ethylenediaminetetraacetic Acid

Dornfeld

Owczarzak

1958

The Journal of Cell Biology

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Cultures of chick heart fibroblasts were perfused with the chelating agent ethylenediaminetetmacetic acid (EDTA). Cellular responses were observed under phase optics and recorded by time-lapse cinemicrography.In interphasic fibroblasts, EDTA induces cellular contraction followed by continuous protrusion and retraction of ectoplasmic blebs ("surface bubbling"), formation of motile vermiform processes, and production of rotatory ectoplasmic swellings. The contraction and surface bubbling closely resemble the metaphase contraction and "anaphase bubbling" normally displayed by cultured fibroblasts.In dividing cells, EDTA does not affect metaphases, but anaphase bubbling appears and persists; telophasic expansion and migration of daughter cells are prevented. Initiation of new mitoses occurs during and after exposure to EDTA.No cellular responses are induced by caldum, magnesium, or ferrous chelates of EDTA. The EDTA effects are completely reversible on removal of the chelating agent, resulting in the restoration of the normal interphasic cell form and the normal expansion and migration of mitotic products.The EDTA effects are interpreted to result from the chelation and removal of divalent cations from the cell surface. Possible relations to surface activities observed in normal mitosis are considered, and an hypothesis is presented regarding the role of the developing spindle in cation transfer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surface Responses in Cultured Fibroblasts Elicited by Ethylenediaminetetraacetic Acid

Dornfeld

Owczarzak

1958

The Journal of Cell Biology

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“…Biochemical evidence relating to the mechanism of mitotic movements is fragmentary and is influenced to a great extent by the classical hypothesis that the mitotic apparatus (or the dividing cell as a whole) is an analogue of a muscle (66). The most spectacular experiments are those of HoffmannBerling (40, 41), who makes "models " of dividing cells by extracting them with cold glycerol, just as working models of muscle fibers are made by glycerol extraction.…”

Section: Chromosome Movementmentioning

confidence: 98%

Biochemistry of the Dividing Cell

Mazia¹

1961

Annu. Rev. Biochem.

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The normal form of cell reproduction involves the duplication of the nu clear genetic material, the partitioning of the genetic material into two equiv alent, or "sister," nuclei, and the partitioning of the cytoplasm into two separate cells, each with one of the nuclei, Nuclear division is not necessarily followed by cytoplasmic division ; the production of multinucleated cells is a common occurrence in nature and in experiments. On the other hand, cell division without nuclear division, which would produce anucleate cytoplasmic masses, is a rare occurrence, obtainable only under special experimental con ditions (36,70,159).In all plant and animal celIs, in protozoa, and in a great many algae and fungi, ceIl division involves the method of mitosis. The defining features of the mitotic method are: the "packaging" of the genetic units in chromo somes, the condensation of the chromosomes at the time of division, and the transportation of sister chromosomes to opposite poles. Thus, the major problems of mitosis are problems of structure and of very specific movement; therefore, a discussion of the biochemistry of the dividing cell takes place in a different context from discussions of metabolic biochemistry and biosyn thesis. It involves the polarization of the whole ceIl, specific "connections" or "attractions" between large bodies, large-scale movements, and, normally, an exact partitioning of the entire cell.Unfortunately, we do not have an adequate account of the method of nuclear division in bacteria and blue-green algae in the sense that mitosis is the method employed by plant and animal cells.

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