Zebrafish twist2/dermo1 regulates scale shape and scale organization during skin development and regeneration

Jacob, Tressa; Chakravarty, Antara; Panchal, Ankita; Patil, Manjiri; Ghodadra, Gautami; Sudhakaran, Jyotish; Nuesslein-Volhard, Christiane

doi:10.1016/j.cdev.2021.203684

Cited by 11 publications

(21 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that the wave is propagated through an intermediary molecule that is expressed in the epidermis and active in the dermis. A strong candidate to participate in the wave is Wnt/β-catenin signaling, which is required for osteoblast differentiation, is active in the epidermis and dermis during scale development, and is capable of activating Eda in other systems 1,26,27 .…”

Section: Resultsmentioning

confidence: 99%

“…To investigate the potential role of Wnt/β-catenin signaling in this process, we used the pharmacological inhibitor IWR-1-endo, which has been shown previously to affect scale development in juvenile zebrafish 1,26,28 . Similar to blocking NF-κB activity, inhibition of Wnt signaling halted scale formation and the formation of new NF-κB placodes.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

An active traveling wave of Eda/NF-kB signaling controls the timing and hexagonal pattern of skin appendages in zebrafish

Evanitsky

Talia

2023

Preprint

View full text Add to dashboard Cite

Periodic patterns make up a variety of tissues, including skin appendages such as feathers and scales. Skin appendages serve important and diverse functions across vertebrates, yet the mechanisms that regulate their patterning are not fully understood. Here, we have used live imaging to investigate dynamic signals regulating the ontogeny of zebrafish scales. Scales are bony skin appendages which develop sequentially along the anterior-posterior and dorsal-ventral axes to cover the fish in a hexagonal array. We have found that scale development requires cell-cell communication and is coordinated through an active wave mechanism. Using a live transcriptional reporter, we show that a wave of Eda/NF-κB activity precedes scale initiation and is required for scale formation. Experiments decoupling the propagation of the wave from dermal placode formation and osteoblast differentiation demonstrate that the Eda/NF-kB activity wavefront times the sequential patterning of scales. Moreover, this decoupling resulted in defects in scale size and significant deviations in the hexagonal patterning of scales. Thus, our results demonstrate that a biochemical traveling wave coordinates scale initiation and proper hexagonal patterning across the fish body.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

An active traveling wave of Eda/NF-kB signaling controls the timing and hexagonal pattern of skin appendages in zebrafish

Evanitsky

Talia

2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The WNT signaling pathway is an evolutionarily conserved intercellular communication system that is important for cell proliferation and differentiation during both development and tissue homeostasis (Steinhart and Angers, 2018). In embryonic development, canonical WNT signaling is required and sufficient for the induction of Twist2 expression in dermal fibroblasts (Budnick et al, 2016;Jacob et al, 2021). In HF morphogenesis, active WNT signaling in dermal fibroblasts through b-catenin and WNT co-TFs, such as Lef1, Tcf1, and Tcf4, is an essential prerequisite for DP commitment (Chen et al, 2012;Gupta et al, 2019;Mok et 2019;Phan et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Twist2-driven chromatin remodeling governs the postnatal maturation of dermal fibroblasts

et al. 2022

View full text Add to dashboard Cite

“…Hormesis drives biological plasticity in a graded response, through the integration of multiple signals and molecular pathways. They are present in organisms that have a preserved proliferative capacity in most of their tissues (invertebrates, fish and amphibians) (Banjac et al, 2023;Blanchoud et al, 2018;Cadiz & Jonz, 2020;Ceci et al, 2018;Chang et al, 2009;Ereskovsky et al, 2021;Guenin-Mace et al, 2023;Huppert & Schwartz, 2023;Imperadore & Fiorito, 2018;Jacob et al, 2021; Regeneration is under hormetic control and damage to tissues initiates a response to restore the equilibrium, whereas repair is a homeorhetic change leading to a new stable stationary state with a decreased functionality. Entrance of tissues or organs into regeneration or repair depends on external balance, changing environmental conditions and signalling pathways for which regulation has evolved in different species.…”

Section: Homeostasis and Homeodynamics In Cell Replacement And Tissue...mentioning

confidence: 99%

Homeostasis and evolution in relation to regeneration and repair

Cano‐Martínez,

Rubio‐Ruiz,

Guarner‐Lans

2024

The Journal of Physiology

View full text Add to dashboard Cite

Homeostasis constitutes a key concept in physiology and refers to self‐regulating processes that maintain internal stability when adjusting to changing external conditions. It diminishes internal entropy constituting a driving force behind evolution. Natural selection might act on homeostatic regulatory mechanisms and control mechanisms including homeodynamics, allostasis, hormesis and homeorhesis, where different stable stationary states are reached. Regeneration is under homeostatic control through hormesis. Damage to tissues initiates a response to restore the impaired equilibrium caused by mild stress using cell proliferation, cell differentiation and cell death to recover structure and function. Repair is a homeorhetic change leading to a new stable stationary state with decreased functionality and fibrotic scarring without reconstruction of the 3‐D pattern. Mechanisms determining entrance of the tissue or organ to regeneration or repair include the balance between innate and adaptive immune cells in relation to cell plasticity and stromal stem cell responses, and redox balance. The regenerative and reparative capacities vary in different species, distinct tissues and organs, and at different stages of development including ageing. Many cell signals and pathways play crucial roles determining regeneration or repair by regulating protein synthesis, cellular growth, inflammation, proliferation, autophagy, lysosomal function, metabolism and metalloproteinase cell signalling. Attempts to favour the entrance of damaged tissues to regeneration in those with low proliferative rates have been made; however, there are evolutionary constraint mechanisms leading to poor proliferation of stem cells in unfavourable environments or tumour development. More research is required to better understand the regulatory processes of these mechanisms. image

show abstract

Zebrafish twist2/dermo1 regulates scale shape and scale organization during skin development and regeneration

Cited by 11 publications

References 53 publications

An active traveling wave of Eda/NF-kB signaling controls the timing and hexagonal pattern of skin appendages in zebrafish

An active traveling wave of Eda/NF-kB signaling controls the timing and hexagonal pattern of skin appendages in zebrafish

Twist2-driven chromatin remodeling governs the postnatal maturation of dermal fibroblasts

Homeostasis and evolution in relation to regeneration and repair

Contact Info

Product

Resources

About