Zebrafish ftz-f1 gene has two promoters, is alternatively spliced, and is expressed in digestive organs

Lin, Wen; Wang, Hsin Wei; Sum, Cristina; Liu, Dong; Hew, Choy L.; Chung, Bon Chu

doi:10.1042/bj3480439

Cited by 25 publications

(23 citation statements)

References 35 publications

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“…Alternative promoter usage has also been observed in the FF1 gene which encodes the zebrafish orthologue of mLRH-1 [17]. Together with our findings, an important question arises: does human LRH-1 gene employ a similar regulatory mechanism to produce variant(s) during embryogenesis and in some adult tissues?…”

Section: Conserved Expression Of the Novel Transcript In Ratssupporting

confidence: 79%

Expression of mouse liver receptor homologue 1 in embryonic stem cells is directed by a novel promoter

et al. 2006

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Liver receptor homologue 1 (LRH-1) plays important roles in many physiological processes and embryogenesis. However, little is known about the developmental regulation of lrh-1 expression. We identified a novel transcript of mouse lrh-1 (mlrh1v2) from embryonic stem (ES) cells. mlrh-1v2 is expressed throughout embryogenesis and in several adult tissues, while the known transcript (mlrh-1v1) appears later during embryogenesis. mlrh-1v2 expression is directed by a new promoter which displays a strong activity in ES cells. The generation of the new transcript is conserved in rats. The identification of novel mlrh-1 variant and promoter is critical for elucidating LRH-1 functions in development and adult tissues.

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Section: Conserved Expression Of the Novel Transcript In Ratssupporting

confidence: 79%

Expression of mouse liver receptor homologue 1 in embryonic stem cells is directed by a novel promoter

et al. 2006

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“…In mammals, expression of SF-1 is essential for the development of gonadotrope of pituitary and VMN of hypothalamus (Parker and Schimmer,'97). In fish, FTZ-F1 homologues were found, by the whole mount in situ hybridization method, to express in the pituitary gland and hypothalamus during embryogenesis in zebrafish (Chai and Chan, 2000;Lin et al, 2000;von Hofsten et al, 2001). RT-PCR analysis indicated that expression of zebrafish zFF1A started from segmentation stage (Lin et al, 2000).…”

Section: Ftz-f1 Homologue In Orange-spotted Groupermentioning

confidence: 99%

cDNA cloning and mRNA expression of a FTZ‐F1 homologue from the pituitary of the orange‐spotted grouper, epinephelus coioides

Zhang

et al. 2004

J. Exp. Zool.

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A FTZ-F1 homologue was cloned from the pituitary cDNA library of the orange-spotted grouper. The full-length cDNA of the orange-spotted grouper FTZ-F1 spanned 1735 bp including a poly (A) tail. The open reading frame encodes a protein of 468 amino acids. Sequence analysis indicated that it had a structure typical of the orphan nuclear receptor superfamily, and the FTZ-F1 box, a characteristic of the FTZ-F1 family. Phylogenetic analysis indicated that the orange-spotted grouper FTZ-F1 was closely related to medaka FTZ-F1 and did not belong to either the SF-1/Ad4BP group or the LRH-1/FTF group. Virtual Northern Blot detected a major transcript of about 1.7 kb and a minor transcript of 2.2 kb of FTZ-F1 in the orange-spotted grouper pituitary gland. The expression of FTZ-F1 homologue gene in different tissues and during embryonic development of the orange-spotted grouper was determined using one-step RT-PCR coupled with Southern blot analysis. In addition to the pituitary gland, the orange-spotted grouper FTZ-F1 was also expressed in the hypothalamus, forebrain, heart, liver, kidney, and ovary. The stronger signal from the gel image indicated that the expression level of FTZ-F1 homologue gene was higher in the ovary of stage 3 than stage 2. During embryonic development, mRNA for the orange-spotted grouper FTZ-F1 homologue was present in newly fertilized eggs, but disappeared in embryos at 50 min post fertilization. The orange-spotted grouper FTZ-F1 homologue mRNA reappeared in embryos at 1.5 hr post fertilization. Its expression level was increased from late blastula to neurula stages. Taken together, results of the current study suggest that the orange-spotted grouper FTZ-F1 homologue exhibits characteristics indicative of both the LRH-1/FTF- and the SF-1/Ad4BP-like genes, and may also play important roles in the hypothalamus-pituitary-gonadal axis, cholesterol metabolism, and embryogenesis.

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“…Within the coding region the intron–exon structure of Smftz‐f1 is conserved in relation to members of the NR5A2 family (including human FTF/LRH (accession no. NT_021968) and zebrafish ff1 [19]) and the NR5A1 family such as the mouse ELP (SF‐1) gene [56], with three of six intron positions conserved. When compared to the D. melanogaster ftz‐f1 gene (accession no. )…”

Section: Organization Of the Smftz‐f1 Gene And Alternative Promoter Umentioning

confidence: 99%

A functionally conserved member of the FTZ‐F1 nuclear receptor family from Schistosoma mansoni

Mendonça

Bouton

Bertin

et al. 2002

European Journal of Biochemistry

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The fushi tarazu factor 1 (FTZ-F1) nuclear receptor subfamily comprises orphan receptors with crucial roles in development and sexual differentiation in vertebrates and invertebrates. We describe the structure and functional properties of an FTZ-F1 from the platyhelminth parasite of humans, Schistosoma mansoni, the first receptor from this family to be characterized in a Lophotrochozoan. It contains a well conserved DNA-binding domain (55-63% identity to other family members) and a poorly conserved ligandbinding domain (20% identity to that of zebrafish FF1a). However, both the ligand domain signature sequence and the activation function 2-activation domain (AF2-AD) are perfectly conserved. Phylogenetic analysis confirmed that SmFTZ-F1 is a member of nuclear receptor subfamily 5, but that it clustered with the Drosophila receptor DHR39 and has consequently been named NR5B1. The gene showed a complex structure with 10 exons and an overall size of 18.4 kb. Two major transcripts were detected, involving alternative promoter usage and splicing of the two 5¢ exons, but which encoded identical proteins. SmFTZ-F1 mRNA is expressed at all life-cycle stages with the highest amounts in the larval forms (miracidia, sporocysts and cercariae). However, expression of the protein showed a different pattern; low in miracidia and higher in adult male worms. The protein bound the same monomeric response element as mammalian SF-1 (SF-1 response element, SFRE) and competition experiments with mutant SFREs showed that its specificity was identical. Moreover, SmFTZ-F1 transactivated reporter gene transcription from SFRE similarly to SF-1. This functional conservation argues for a conserved biological role of the FTZ-F1 nuclear receptor family throughout the metazoa.Keywords: platyhelminth; development; orphan receptor; phylogeny; DNA-binding.The FTZ-F1 gene subfamily encodes orphan nuclear receptors and appears to be present in all metazoan phyla [1]. The first member of the subfamily, FTZ-F1a, was isolated from Drosophila melanogaster [2,3] and was identified both as a transcriptional regulator and cofactor [4,5] of the homeodomain protein fushi tarazu (FTZ), a segmentation gene of the pair-rule class responsible for the formation of alternative segmental units in the D. melanogaster embryo [6]. FTZ-F1a is expressed in early embryos, concomitant with FTZ expression. A second isoform, FTZ-F1b, encoded by the same gene [7], is detectable in late-stage embryos through to adults, when FTZ expression is absent, and regulates genes associated with ecdysis and metamorphosis [8]. In the nematode Caenorhabditis elegans, nhr-25, the homologue of FTZ-F1, is required for epidermal and somatic gonad development and also participates in the regulation of moulting [9,10]. In vertebrates, an FTZ-F1 orthologue was first identified as a steroidogenic factor (Ad4BP/SF-1) present in the adrenal gland and able to bind to proximal promoter regions of cytochrome P450 steroid hydroxylase genes (reviewed in [11]). Further studies performed to identify the t...

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Zebrafish ftz-f1 gene has two promoters, is alternatively spliced, and is expressed in digestive organs

Cited by 25 publications

References 35 publications

Expression of mouse liver receptor homologue 1 in embryonic stem cells is directed by a novel promoter

Expression of mouse liver receptor homologue 1 in embryonic stem cells is directed by a novel promoter

cDNA cloning and mRNA expression of a FTZ‐F1 homologue from the pituitary of the orange‐spotted grouper, epinephelus coioides

A functionally conserved member of the FTZ‐F1 nuclear receptor family from Schistosoma mansoni

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