cDNA cloning and mRNA expression of a FTZ‐F1 homologue from the pituitary of the orange‐spotted grouper, <i>epinephelus coioides</i>

Zhang, Weiyang; Li, Xin; Zhang, Yong; Zhang, Lihong; Tian, Jing; Ma, Guangzhi

doi:10.1002/jez.a.74

Cited by 17 publications

(4 citation statements)

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“…Multiple forms of FTZ-F1 have been reported across vertebrate phyla that includes four isoforms in zebrafish [15] , [21] , [31] and two isoforms in the arctic char [17] , [32] , frog [33] and chicken [27] . In the present study, only a single form of FTZ-F1 was obtained similar to rainbow trout [25] , medaka [18] and orange spotted grouper [34] . The deduced amino acid sequence and phylogenetic analysis of catfish FTZ-F1 showed identity with NR5A1 genes.…”

Section: Discussioncontrasting

confidence: 60%

Molecular Cloning and Expression Analysis of fushi tarazu Factor 1 in the Brain of Air-Breathing Catfish, Clarias gariepinus

2011

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Background Fushi tarazu factor 1 (FTZ-F1) encodes an orphan nuclear receptor belonging to the nuclear receptor family 5A (NR5A) which includes adrenal 4-binding protein or steroidogenic factor-1 (Ad4BP/SF-1) and liver receptor homologue 1 (LRH-1) and plays a pivotal role in the regulation of aromatases.Methodology/Principal FindingsPresent study was aimed to understand the importance of FTZ-F1 in relation to brain aromatase (cyp19a1b) during development, recrudescence and after human chorionic gonadotropin (hCG) induction. Initially, we cloned FTZ-F1 from the brain of air-breathing catfish, Clarias gariepinus through degenerate primer RT-PCR and RACE. Its sequence analysis revealed high homology with other NR5A1 group members Ad4BP/SF-1 and LRH-1, and also analogous to the spatial expression pattern of the latter. In order to draw functional correlation of cyp19a1b and FTZ-F1, we analyzed the expression pattern of the latter in brain during gonadal ontogeny, which revealed early expression during gonadal differentiation. The tissue distribution both at transcript and protein levels revealed its prominent expression in brain along with liver, kidney and testis. The expression pattern of brain FTZ-F1 during reproductive cycle and after hCG induction, in vivo was analogous to that of cyp19a1b shown in our earlier study indicating its involvement in recrudescence.Conclusions/SignificanceBased on our previous results on cyp19a1b and the present data, it is plausible to implicate potential roles for brain FTZ-F1 in ovarian differentiation and recrudescence process probably through regulation of cyp19a1b in teleosts. Nevertheless, these interactions would require primary coordinated response from ovarian aromatase and its related transcription factors.

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Section: Discussioncontrasting

confidence: 60%

Molecular Cloning and Expression Analysis of fushi tarazu Factor 1 in the Brain of Air-Breathing Catfish, Clarias gariepinus

2011

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“…NR5A1 are key regulators of steroidogenesis and are involved in the testis determining pathway (Ikeda et al 1993(Ikeda et al , 1994Sadovsky et al 1995). A FTZ-F1 homologue was cloned from the pituitary cDNA library of the Fish Physiol Biochem (2010) 36:181-193 189 orange-spotted grouper (Zhang et al 2004b). The expression of FTZ-F1 in the gonad decreased significantly in response to MT treatment for 40 days and 60 days, but its expression in the pituitary was not altered significantly.…”

Section: Molecular Mechanisms On Sex Change In Groupersmentioning

confidence: 99%

Molecular mechanisms underlying sex change in hermaphroditic groupers

Zhou

Gui

2008

Fish Physiol Biochem

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Groupers are widely distributed throughout the tropical and subtropical waters of the world and are regarded as a favourite marine food fish. However, their large-scale aquaculture has been hindered by the rarity of natural males. Being protogynous hermaphrodites, groupers have been considered as study model for development and reproduction, especially for sex determination or sex differentiation, owing to the advantage that grouper gonad development undergoes transition from ovary to intersexual gonad and then to testis, and primordial germ cells and different stages of gametic cells during oogenesis and spermatogenesis are synchronously observed in the transitional gonads. Recently, a series of genes related to the reproduction regulation or sex differentiation have been identified in the groupers, mainly by researchers in China. One important finding was that the grouper gene, doublesex/male abnormal 3-related transcription factor 1 (DMRT1), is not only differentially expressed in gonads at different stages, but that it is also restricted to specific stages and specific cells of spermatogenesis. Grouper DMRT1 protein exists only in spermatogonia, primary spermatocytes and secondary spermatocytes, but not in the supporting Sertoli cells. Moreover, no introns were found in the grouper DMRT1, and no duplicated DMRT1 genes were detected. The finding implies that the intronless DMRT1 that is able to undergo rapid transcriptional turnover might be a significant gene for stimulating spermatogenesis in the protogynous hermaphroditic gonad. Additionally, we have found that grouper expression of sex-determining region Y-related highmobility group-box gene 3 (SOX3) is a significant time point for enterable gametogenesis of primordial germ cells, because SOX3 is obviously expressed and localized in primordial germ cells. As SOX3 continues to express, the SOX3-positive primordial germ cells develop toward oogonia and then oocytes, whereas, when SOX3 expression is ceased, the SOX3-positive primordial germ cells develop toward spermatogonia. Therefore, we suggest that SOX3, as a transcription factor, might have more important roles in oogenesis than in spermatogenesis. Based on the findings, a hypothetic molecular mechanism underlying sex change is proposed in the hermaphroditic groupers, and some candidate genes related to the grouper sex change are also suggested for further research.

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“…Our previous study showed that the orange-spotted grouper cyp19a1a promoter had two conserved Ftz-f1 binding sites, which could mediate the activation of cyp19a1a promoter by Ftz-f1 homologues [18]. However, a previously isolated Ftz-f1 homologue (Nr5a4) from the orangespotted grouper [19] could not activate cyp19a1a transcription in vitro [18]. In the present study, a Lrh-1 homologue is isolated from the orange-spotted grouper, and its possible involvement in the regulation of cyp19a1a in the ovary is explored.…”

Section: Introductionmentioning

confidence: 99%

Cytoplasmic Localization of Lrh-1 Down-Regulates Ovarian Follicular cyp19a1a Expression in a Teleost, the Orange-Spotted Grouper Epinephelus coioides1

Zhang

Liu

et al. 2014

Biology of Reproduction

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Liver receptor homolog-1 (LRH-1) is a conserved member of the NR5A subfamily in vertebrates and a potential regulator of estrogen synthesis in the ovarian granulosa cells. An Lrh-1 homologue was obtained from the orange-spotted grouper Epinephelus coioides that contains the conserved structural features of NR5A and is phylogenetically closely related to NR5A2. The expression of the orange-spotted grouper Lrh-1 is tissue-specific with relatively higher levels in the liver and ovary. The immunoreactive signals for Lrh-1 and Cyp19a1a were present in the ovarian follicular cells and germ cells. In the ovarian follicular cells, Lrh-1 was present both in the nucleus and cytoplasm, and colocalized with Cyp19a1a. The expression levels of both increased during vitellogenesis whereas only Cyp19a1a dramatically decreased toward maturation when Lrh-1 was localized almost exclusively to the cytoplasm of the follicular cells. The orange-spotted grouper Lrh-1 could upregulate cyp19a1a transcription in vitro via the two conserved Ftz-f1 sites in cyp19a1a promoter. Chromatin immunoprecipitation analysis showed that the orange-spotted grouper Lrh-1 could bind cyp19a1a promoter in vivo with a higher abundance in the vitellogenic ovary, whereas the binding was dramatically decreased in the mature ovary. Taken together, the results of present study demonstrate that Lrh-1 plays an important role in up-regulating cyp19a1a gene in the ovarian follicular cells during vitellogenesis, and the sequestration of Lrh-1 to the cytoplasm may down-regulate cyp19a1a expression in the mature ovary. This mechanism for modifying transcriptional roles of the orange-spotted grouper Lrh-1 may shed new light on the regulation of Cyp19a1 expression in other vertebrates as well.

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cDNA cloning and mRNA expression of a FTZ‐F1 homologue from the pituitary of the orange‐spotted grouper, epinephelus coioides

Cited by 17 publications

References 29 publications

Molecular Cloning and Expression Analysis of fushi tarazu Factor 1 in the Brain of Air-Breathing Catfish, Clarias gariepinus

Molecular Cloning and Expression Analysis of fushi tarazu Factor 1 in the Brain of Air-Breathing Catfish, Clarias gariepinus

Molecular mechanisms underlying sex change in hermaphroditic groupers

Cytoplasmic Localization of Lrh-1 Down-Regulates Ovarian Follicular cyp19a1a Expression in a Teleost, the Orange-Spotted Grouper Epinephelus coioides1

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