Zebrafish developmental toxicity assay: A fishy solution to reproductive toxicity screening, or just a red herring?

Teratological Effects of a Panel of Sixty Water-Soluble Toxicants on Zebrafish Development

“…Examples of this application include the toxicity testing of nanoparticles [31][32][33][34] and chemical compounds from different pharmacological classes, 11 and developmental toxicity testing of ethanol [35][36][37] and other compounds. 13,14,[38][39][40][41][42][43][44][45][46][47] There are several advantages of using zebrafish embryos in biomedical research (reviewed in Ali et al…”

Influence of the perivitelline space on the quantification of internal concentrations of chemicals in eggs of zebrafish embryos (Danio rerio)

“…But exposure experiments for the analytical investigation of internal concentrations quite often start with ZFE of an older age of 72 h post-fertilization (hpf) (El-Amrani et al, 2012Gonzalo-Lumbreras et al, 2012;Jones et al, 2012). If experiments are performed at early life-stages of the embryo the first samples are often taken after 72 hpf (Bluthgen et al, 2012;Van den Bulck et al, 2011). However, it would be worthwhile to cover also very early life-stages of ZFE (<72 hpf) to learn about the uptake and metabolism of different study compounds in these early stages of development, in which the ZFE did not hatch.…”

Alternative models in developmental toxicology

“…Another recent zebrafish assay was described by Van den Bulck et al [2011]. These authors treated zebrafish from the sphere stage until 96 hpf; at the conclusion of the culture, 28 morphological endpoints were evaluated.…”

“…The role of strain differences in sensitivity of zebrafish to compounds also has not been examined. Van den Bulck et al [2011] attempted to address, in part, the uptake of compounds by zebrafish. At the lowest observed effect concentration or the NOAEL, larvae were processed to quantitate the amount of compound in the larvae.…”